Acta Vet. Brno 2019, 88: 169-175
https://doi.org/10.2754/avb201988020169
Flow cytometry in assessment of sperm integrity and functionality – a review
References
1. N, Finak G, Hoos H, Mosmann TR, Brinkman R, Gottardo R, Scheuermann RH 2013: Critical assessment of automated flow cytometry data analysis techniques. Nat Methods 10: 228-238
<https://doi.org/10.1038/nmeth.2365>
2. M, He L, Buhr MM, Kroetsch TG, Pauls KP 2002: Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility. Biol Reprod 66: 354-360
<https://doi.org/10.1095/biolreprod66.2.354>
3. M, Lornage J, Mazoyer C, Lejeune H, Salle B, Guerin FJ 2007: Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril 87: 93-100
<https://doi.org/10.1016/j.fertnstert.2006.05.057>
4. JF, Gadella BM 2003: In situ detection and localization of lipid peroxidation in individual bovine sperm cells. Free Radic Biol Med 35: 1382-1391
<https://doi.org/10.1016/j.freeradbiomed.2003.08.010>
5. L, Sabeur K, Ball BA 2007: Generation of superoxide anion by equine spermatozoa as detected by dihydroethidium. Theriogenology 67: 580-589
<https://doi.org/10.1016/j.theriogenology.2006.07.021>
6. DT, Liu L, Peterson CM, Jones KP, Hatasaka H, Erickson L, Campbell B 2003: Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch Androl 49: 49-55
<https://doi.org/10.1080/01485010290099390>
7. CCE, de Andrade CFA, Raphael FC, Nascimento J, Ticianelli SJ, de Arruda PR 2010: Damage assessment of the equine sperm membranes by fluorimetric technique. Braz Arch Biol Technol 53: 1285-1292
<https://doi.org/10.1590/S1516-89132010000600004>
8. J, Dodani SC, Chang CJ 2012: Reaction-based small-molecule fluorescent probes for chemoselective bioimaging. Nat Chem 4: 973-984
<https://doi.org/10.1038/nchem.1500>
9. Y, James PS, Jones R 2004: Lipid diffusion in sperm plasma membranes exposed to peroxidative injury from oxygen free radicals. Mol Reprod Dev 68: 365-372
<https://doi.org/10.1002/mrd.20084>
10. E, Eleuteri P, Leter G, Rescia M, Spanó M 2005: Flow cytometry applications in the evaluation of sperm quality: semen analysis, sperm function and DNA integrity. Contraception 72: 273-279
<https://doi.org/10.1016/j.contraception.2005.03.004>
11. ATM, Carvalho JO, Dode MAN 2015: Techniques for sperm evaluation using fluorescent probes. Semin Ciênc Agrár 36: 4365-4376
<https://doi.org/10.5433/1679-0359.2015v36n6Supl2p4365>
12. Dolník M 2018: Effect of selenium on the fertilizing characteristics of ruminant spermatozoa (dissertation), 95 p.
13. M, Pošivák J, Kadáši M, Klein R, Lazar G, Mudroňová D 2018: Evaluation of the sperm fertilization status by flow cytometry. Magyar Állatorvosok Lapja 140: 337-341
14. D, Camejo MI, Ballesca JL, Oliva R 2007: Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy. Cytometry A 71A: 1011-1018
<https://doi.org/10.1002/cyto.a.20484>
15. GP, Gadella BM, Post JA, Brouwers F 2004: Mass spectrometric characterization of the oxidation of the fluorescent lipid peroxidation reporter molecule C11-BODIPY. Free Radic Biol Med 36: 1635-1644
<https://doi.org/10.1016/j.freeradbiomed.2004.03.014>
16. DP, Darzynkiewicz Z, Melamed MR 1980: Comparison of human and mouse sperm chromatin structure by flow cytometry. Chromosoma 78: 225-238
<https://doi.org/10.1007/BF00328394>
17. DP, Wixon R 2006: Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology 65: 979-991
<https://doi.org/10.1016/j.theriogenology.2005.09.011>
18. K, Sekino M, Hata A, Ikebuchi R, Nakanishi Y, Egawa G, Kabashima K, Watanabe T, Furuki M, Tomura M 2015: Novel full-spectral flow cytometry with multiple spectrally-adjacent fluorescent proteins and fluorochromes and visualization of in vivo cellular movement. Cytometry A 87: 830-842
<https://doi.org/10.1002/cyto.a.22725>
19. O, Maroto-Morales A, Martínez-Pastor F, Garde JJ, Ramón M, Fernández-Santos RM, Esteso CM, Pérez-Guzmán DM, Soler JA 2009: Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: Electroejaculation and postmortem collection. Theriogenology 72: 160-168
<https://doi.org/10.1016/j.theriogenology.2009.02.002>
20. DL, Johnson LA, Yue ST, Roth BL, Haugland RP 1994: Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide. J Androl 15: 620-629
21. JM, Zweifel UL, Peters F, Fuhrman JA, Hagstrom A 1999: Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria. Appl Environ Microbiol 65: 4475-4483
22. L, Evans G, Maxwell WM 2005: Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63: 445-457
<https://doi.org/10.1016/j.theriogenology.2004.09.024>
23. A, Lindstedt L, Larsson M, Bungum M, Spano M, Levine RJ, Rylander L 2010: Sperm chromatin structure assay as an independent predictor of fertility in vivo: a case–control study. Int J Androl 33: 221-227
<https://doi.org/10.1111/j.1365-2605.2009.00995.x>
24. GA, Hendry JH, Daniel CP, Morris ID 2002: Germ cell and dose-dependent DNA damage measure by the comet assay in murine spermatozoa after testicular X-irradiation. Biol Reprod 67: 854-861
<https://doi.org/10.1095/biolreprod.102.004382>
25. MS, Johannisson A, Wallgren M, Nagy S, Siqueira AP, Rodriguez-Martinez H 2011: Flow cytometry for the assessment of animal sperm integrity and functionality: state of art. Asian J Androl 13: 406-419
<https://doi.org/10.1038/aja.2011.15>
26. T, Flores-Montero J, van der Velden VH, Martin-Ayuso M, Bottcher S, Ritgen M, Almeida J, Lhermitte L, Asnafi V, Mendonca A, de Tute R, Cullen M, Sedek L, Vidriales MB, Pérez JJ, te Marvelde JG, Mejstrikova E, Hrusak O, Szczepański T, van Dongen JJM, Orfao A 2012: Euroflow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia 26: 1986-2010
<https://doi.org/10.1038/leu.2012.122>
27. DS, Evenson DP, Jost LK, Baer RK 1990: Comparison of semen quality in young and mature Holstein bulls measured by light microscopy and flow cytometry. J Dairy Sci 73: 2364-2371
<https://doi.org/10.3168/jds.S0022-0302(90)78919-9>
28. T, Ogawa K, Mizuno K, Nagai S, Uchida Y, Ohta S, Fujie M, Suzuki K, Hirata S, Hoshi K 2002: Relationship between mitochondrial membrane potential, sperm motility and fertility potential. Asian J Androl 4: 97-103
29. SH, Yu DH, Kim YJ 2010: Apoptosis-like change, ROS, and DNA status in cryopreserved canine sperm recovered by glass wool filtration and Percoll gradient centrifugation techniques. Anim Reprod Sci 119: 106-116
<https://doi.org/10.1016/j.anireprosci.2009.11.002>
30. AJ, De Luliis GN, Finnie JM, McLaughlin EA, Aitken J 2008: Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab 93: 3199-3207
<https://doi.org/10.1210/jc.2007-2616>
31. P, Danguy A, Leleux F, Meuris S, Lebrun P 2009: Improved methodology for the detection and quantification of the acrosome reaction in mouse spermatozoa. Histol Histopathol 24: 999-1007
32. M, Guitton E, Levern Y, Nicolle ClJ, Vidament M, Kerboeuf D, Palmer E 1997: New staining methods for sperm evaluation estimated by microscopy and flow cytometry. Theriogenology 48:1229-1235
<https://doi.org/10.1016/S0093-691X(97)00355-5>
33. R, Said MT, Agarwal A 2009: The diagnostic and therapeutic applications of flow cytometry in male infertility. Arch Med Sci 5: 99-108
34. E, Pérez-Pé R, Muino-Blanco T, Cebrián-Pérez JA 2006: Comparative study of four different sperm washing methods using apoptotic markers in ram spermatozoa. J Androl 27: 746753
<https://doi.org/10.2164/jandrol.106.000109>
35. F, Mata-Campuzano M, Álvarez-Rodríguez M, Álvarez M, Anel L, de Paz P 2010: Probes and techniques for sperm evaluation by flow cytometry. Reprod Domest Anim 45: 67-78
<https://doi.org/10.1111/j.1439-0531.2010.01622.x>
36. S, Jansen J, Topper EK, Gadella BM 2003: A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod 68: 1828-1865
<https://doi.org/10.1095/biolreprod.102.011445>
37. H, Blässe AK, Wolkers WF, Bollwein H, Sieme H 2011: Osmotic properties of stallion sperm subpopulations determined by simultaneous assessment of cell volume and viability. Theriogenology 76: 386-391
<https://doi.org/10.1016/j.theriogenology.2011.02.027>
38. FC, Gonzáles FL, Morrell JM, Salazar SC, Macias GB, Rodríguez-Martinez H, Tapia JA, Peňa FJ 2009: Lipid peroxidation, assessed with BODIPY-C11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like changes. Reproduction 138: 55-63
<https://doi.org/10.1530/REP-08-0484>
39. FJ 2015: Multiparametric flow cytometry: a relevant tool for sperm function evaluation. Anim Reprod 12: 351-355
40. AM, Harrison RA 2011: Cytometric solutions in veterinary andrology: developments, advantages, and limitations. Cytometry A 79: 338-348
<https://doi.org/10.1002/cyto.a.21044>
41. H 2003: Laboratory semen assessment and prediction of fertility: still utopia? Reprod Domest Anim 38: 312-318
<https://doi.org/10.1046/j.1439-0531.2003.00436.x>
42. TL, Roseiro JC, Reis A 2012: Applications and perspectives of multi-parameter flow cytometry to microbial biofuels production processes. Trends Biotechnol 30: 225-232
<https://doi.org/10.1016/j.tibtech.2011.11.005>
43. P, Underwood SL, Dolbec C, Bouchard N, Kroetsch T, Blondin P 2012: Bovine semen quality control in artificial insemination centers. Anim Reprod 9: 153-165
44. Y, Zimmerman SW, Manandhar G, Oddhiambo JF, Kennedy C, Jonáková V, Maňásková-Postlerová P, Sutovský M, Park CS, Sutovsky P 2012: Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm–egg coat penetration during porcine fertilization. Int J Androl 35: 196-210
<https://doi.org/10.1111/j.1365-2605.2011.01217.x>
45. H, Joseph J, Fales HM, Sokoloski EA, Levine RL, Vasquez-Vivar J, Kalyanaraman B 2005: Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci USA 102: 5727-5732
<https://doi.org/10.1073/pnas.0501719102>
