Acta Vet. Brno 2019, 88: 225-232

The effect of triamcinolone in liposomes on oral wound healing in rats

Vladimira Erjavec1, Zlatko Pavlica1, Tomáš Fichtel2, Milan Petelin3

1University of Ljubljana, Veterinary Faculty, Small animal clinic, Ljubljana, Slovenia
2University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Small Animal Clinic, Brno, Czech Republic
3University of Ljubljana, Faculty of Medicine, Department of Oral Medicine and Periodontology, Ljubljana, Slovenia

Received July 23, 2018
Accepted April 23, 2019

The purpose of this study was to determine whether oral mucosa wounds in rats can be successfully treated with triamcinolone acetonide (TA), incorporated into liposomes. A round wound was inflicted on the oral mucosa of female Wistar rats divided into four groups of 12 animals. This wound was treated topically from day 1 with liposomes without the inclusion of TA and liposomes containing 0.01% or 0.05% TA. The wounds of the animals in the control group were not treated. Polymethyl metacrylate was used as an ointment for mixing in liposomes. The size of the wound was measured until day 6. The area of inflammatory infiltrate under the wound was evaluated by histopathology, the expression of iNOS (inducible nitric oxide synthase) enzyme under the wound was evaluated by immunohistochemistry until day 6. On the sixth day of experiment, the size of the wound and the area of the inflammatory infiltrate was the smallest in the group receiving empty liposomes (EL). Expression of iNOS was the most reduced in the group receiving EL. We conclude that oral mucosa wounds can be successfully treated with liposomes, although the incorporated drug triamcinolone would not be the appropriate drug for treating wounds of traumatic origin.


The study was supported by the Slovenian Research Agency, grant No. P4–0053. The authors would also like to thank Dr. Monika Žužek and Dr. Tomaž Büdefeld for providing help during the research.


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