Acta Vet. Brno 2019, 88: 303-306

Co-infection with Angiostrongylus chabaudi and Dirofilaria immitis in a wildcat, Felis silvestris from Romania – a case report

Călin Mircea Gherman1, Angela Monica Ionică1, Georgiana Deak1, Gabriel Bogdan Chișamera2, Andrei Daniel Mihalca1

1University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Veterinary Medicine, Department of Parasitology and Parasitic Diseases, Cluj-Napoca, Romania
2National Museum of Natural History “Grigore Antipa”, Bucharest, Romania

Received March 1, 2019
Accepted June 13, 2019

Dirofilaria immitis is a worldwide spread nematode affecting the pulmonary artery and the heart of dogs (rarely reported in cats), especially in areas where the dogs show a high prevalence of infection. Angiostrongylus chabaudi is, in turn, a cardiopulmonary nematode of felids identified in several southern European countries and Germany. Co-infections of Dirofilaria immitis and Angiostrongylus spp. are known only in canids. We report a case of D. immitis and A. chabaudi co-infection in a road-killed wildcat originating from Southeastern Romania. Overall, 17 nematodes were collected from the pulmonary arteries of the wildcat and were morphologically identified as A. chabaudi (2 males and 15 females). Another nematode was collected from the right ventricle and identified as adult male D. immitis. Genomic DNA was extracted from one nematode of each of the two species and a fragment of the cox1 gene was amplified and sequenced. The sequences obtained from the nematodes showed 100% similarity to a sequence of A. chabaudi isolated from Romania (Accession number KU521521) and to various D. immitis sequences from Europe, Asia and Australia (e.g. KT716014, EU159111, AJ537512). This is the first report of A. chabaudi and D. immitis co-infection in a felid, revealing the possibility of similar situations in domestic cats. This requires a more in-depth clinical and laboratory examination of animals with respiratory and cardiac symptoms.


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