Acta Vet. Brno 2019, 88: 443-449

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

Tomáš Komprda1, Zbyšek Sládek2, Veronika Švehlová1, Zuzana Lacková3,4, Roman Guráň3,4, Tomáš Do4, Jan Wijacki2, Marcela Buchtová5, Jana Neuwirthová6, Břetislav Gál6, Vojtěch Adam3,4, Ondřej Zítka3,4

1Mendel University in Brno, Department of Food Technology, Brno, Czech Republic
2Mendel University in Brno, Department of Animal Morphology, Physiology and Genetics, Brno, Czech Republic
3Brno University of Technology, Central European Institute of Technology, Brno, Czech Republic
4Mendel University in Brno, Department of Chemistry and Biochemistry, Brno, Czech Republic
5Czech Academy of Sciences, Institute of Animal Physiology and Genetics, v.v.i., Brno, Czech Republic
6Masaryk University, St. Anne’s Hospital and Faculty of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, Brno, Czech Republic

Received March 15, 2019
Accepted October 29, 2019


1. Angel PM, Comte-Walters S, Ball LE, Talbot K, Mehta A, Brockbank KGM, Drake RR 2018: Mapping extracellular matrix proteins in formalin-fixed, paraffin-embedded tissues by MALDI imaging mass spectrometry. J Proteome Res 17: 635-646 <>
2. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MAC 2016: Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol 54: 2555-2559 <>
3. Campioli E, Rustichelli C, Avallone R 2012: N-3 dietary supplementation and lipid metabolism: Differences between vegetable- and fish-derived oils. J Funct Foods 41: 207-212 <>
4. Chen WY, Lin SY, Pan HC, Liao SL, Chuang YH, Yen YJ, Chen CJ 2012: Beneficial effect of docosahexaenoic acid on cholestatic liver injury in rats. J Nutr Biochem 23: 252-264 <>
5. dos Santos Rosa A, Bandeira LG, Monte-Alto-Costa A, Romana-Souza B 2014: Supplementation with olive oil, but not fish oil, improves cutaneous wound healing in stressed mice. Wound Repair Regen 22: 537-547 <>
6. Drzymala-Czyz S, Banasiewicz T, Tubacka M, Tarasiuk-Rusek A, Majewski P, Drews M. Walkowiak J 2012: Discrepancy between clinical and histological effects of DHA supplementation in a rat model of pouchitis. Folia Histochem Cyto 50: 125-129 <>
7. Gercek A, Yildirim O, Konya D, Bozkurt S, Ozgen S, Kilic T, Sav A, Pamir N 2007: Effects of parenteral fish-oil emulsion (Omegaven) on cutaneous wound healing in rats treated with dexamethasone. J Parenter Enter Nutr 31: 161-166 <>
8. Guran R, Vanickova L, Horak V, Krizkova S, Michalek P, Heger Z, Zitka O, Adam V 2017: MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles. PLOS ONE 12: e0189305 <>
9. Heger Z, Polanska H, Merlos Rodrigo MA, Guran R, Kulich P, Kopel P, Masarik M, Eckschlager T, Stiborova M, Kizek R, Adam V 2016: Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes. Sci Rep 6: 33379 <>
10. Howe PRC, Buckley JD, Murphy KJ, Pettman T, Milte C, Coates AM 2014: Relationship between erythrocyte omega-3 content and obesity is gender dependent. Nutrients 6: 1850-1860 <>
11. Komprda T 2018: Effect of n-3 polyunsaturated fatty acids on wound healing using animal models – a review. Acta Vet Brno 87: 309-320 <>
12. Komprda T, Rozikova V, Zamazalova N, Skultety O, Vicenova M, Trckova M, Faldyna M 2017: Effect of dietary fish oil on fatty acid deposition and expression of cholesterol homeostasis controlling genes in the liver and plasma lipid profile: comparison of two animal models. J Anim Physiol Anim Nutr 101: 1093-1102 <>
13. Komprda T, Sladek Z, Skultety O, Krizkova S, Rozikova V, Nemcova B, Sustrova T, Valova M 2016: Effect of dietary Schizochytrium microalga oil on selected markers of low-grade inflammation in rats. J Anim Physiol Anim Nutr 100: 1169-1178 <>
14. Lewis EEL, Barrett MRT, Freeman-Parry L, Bojar RA, Clench MR 2018: Examination of the skin barrier repair/wound healing process using a living skin equivalent model and matrix-assisted laser desorption-ionization-mass spectrometry imaging. Int J Cosmetic Sci 40: 148-156 <>
15. Oktay HO, Cenker E, Zeynep A, Filiz O, Mutay A 2018: Mass spectrometric identification of collagen alpha-1 (III) chain and chondroitin sulfate proteoglycan-4 nitration in patients with acute pulmonary embolism. Curr Proteomics 15: 34-41
16. Olson MV, Liu YC, Dangi B, Zimmer JP, Salem N, Nauroth JM 2013: Docosahexaenoic acid reduces inflammation and joint destruction in mice with collagen-induced arthritis. Inflamm Res 62: 1003-1013 <>
17. Otranto M, Do Nascimento AP, Monte-Alto-Costa A 2010: Effects of supplementation with different edible oils on cutaneous wound healing. Wound Repair Regen 18: 629-636 <>
18. Pietrowska M, Gawin M, Polanska J, Widlak P 2016: Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI-IMS. Proteomics 16: 1670-1677 <>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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