The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

Komprda, Tomáš; Sládek, Zbyšek; Švehlová, Veronika; Lacková, Zuzana; Guráň, Roman; Do, Tomáš; Wijacki, Jan; Buchtová, Marcela; Neuwirthová, Jana; Gál, Břetislav; Adam, Vojtěch; Zítka, Ondřej

doi:10.2754/avb201988040443

Acta Veterinaria > Archive > 2019, Vol. 88 > Issue 4 > The effect of different fatty acid…

Acta Vet. Brno 2019, 88: 443-449

https://doi.org/10.2754/avb201988040443

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

Tomáš Komprda¹, Zbyšek Sládek², Veronika Švehlová¹, Zuzana Lacková^3,4, Roman Guráň^3,4, Tomáš Do⁴, Jan Wijacki², Marcela Buchtová⁵, Jana Neuwirthová⁶, Břetislav Gál⁶, Vojtěch Adam^3,4, Ondřej Zítka^3,4

¹Mendel University in Brno, Department of Food Technology, Brno, Czech Republic
²Mendel University in Brno, Department of Animal Morphology, Physiology and Genetics, Brno, Czech Republic
³Brno University of Technology, Central European Institute of Technology, Brno, Czech Republic
⁴Mendel University in Brno, Department of Chemistry and Biochemistry, Brno, Czech Republic
⁵Czech Academy of Sciences, Institute of Animal Physiology and Genetics, v.v.i., Brno, Czech Republic
⁶Masaryk University, St. Anne’s Hospital and Faculty of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, Brno, Czech Republic

Received March 15, 2019
Accepted October 29, 2019

The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen α-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semi-quantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neo-angiogenesis was assessed as S > P > F ≈ Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.

Keywords

Docosahexaenoic acid, eicosapentaenoic acid, Schizochytrium oil, reepithelialization, MALDI MSI, collagen alpha-1(III).

Funding

The experiment was financially supported by the Internal Grant Agency of Mendel University in Brno (project no. TP3/2017) and by CEITEC 2020 (LQ1601).