Acta Vet. Brno 2020, 89: 401-411

Assessment of antibiotic resistance in starter and non-starter lactobacilli of food origin

Marta Dušková1,2, Monika Morávková2, Jakub Mrázek3, Martina Florianová2, Lenka Vorlová1, Renáta Karpíšková1,2

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Origin Food and Gastronomic Sciences, Brno, Czech Republic
2Veterinary Research Institute, Brno, Czech Republic
3Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Prague, Czech Republic

Received June 1, 2020
Accepted December 21, 2020


1. Abriouel H, Muńoz MDC, Lerma LL, Montoro BP, Bockelmann W, Pichner R, Kabisch J, Cho GS, Franz CMAP, Gálvez A, Benomar N 2015: New insights in antibiotic resistance of Lactobacillus species from fermented foods. Food Res Int 78: 465-481 <>
2. Ammor MS, Flórez AB, Mayo B 2007: Antibiotic resistance in non-enterococcal lactic acid bacteria and bifidobacteria. Food Microbiol 24: 559-570 <>
3. Ammor MS, Gueimonde M, Danielsen M, Zagorec M, van Hoek AHAM, de los Reyes-Gavilán CG, Mayo B, Margolles A 2008: Two different tetracycline resistance mechanisms, plasmid-carried tet(l) and chromosomally located transposon-associated tet(M), coexist in Lactobacillus sakei rits 9. Appl Environ Microb 74: 1394-1401 <>
4. Arias CA, Vallejo M, Reyes J, Panesso D, Moreno J, Castaneda E, Villegas MV, Murray BE, Quinn JP 2008: Clinical and microbiological aspects of linezolid resistance mediated by the cfr gene encoding a 23S rRNA methyltransferase. J Clin Microbiol 46: 892-896 <>
5. Atashpaz S, Khani S, Barzegari A, Barar J, Vahed SZ, Azarbaijani R, Omidi Y 2010: A robust universal method for extraction of genomic DNA from bacterial species. Microbiology 79: 538-542 <>
6. Bardon J, Mlynarcik P, Prochazkova P, Roderova M, Mezerova K, Kolar M 2018: Occurrence of bacteria with a dangerous extent of antibiotic resistance in poultry in the Central Region of Moravia. Acta Vet Brno 87: 165-172 <>
7. Bernardeau M, Vernoux JP, Henri-Dubernet S, Guéguen M 2008: Safety assessment of dairy microorganisms. The Lactobacillus genus. Int J Food Microbiol 126: 278-285 <>
8. Chopra I, Roberts M 2001: Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol R 65: 232-260 <>
9. CLSI 2016: Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria (3rd edn). CLSI guideline M45, Wayne, PA: Clinical and Laboratory Standards Institute
10. Curragh HJ, Collins MA 1992: High levels of spontaneous drug resistance in Lactobacillus. J Appl Bacteriol 73: 31-36 <>
11. Dec M, Urban-Chmiel R, Stepien-Pysniak D, Wernicki A 2017: Assessment of antibiotic susceptibility in Lactobacillus isolates from chickens. Gut Pathog 9: e54 <>
12. Devirgiliis C, Zinno P, Perozzi G 2013: Update on antibiotic resistance in foodborne Lactobacillus and Lactococcus species. Front Microbiol 4: e00301 <>
13. Dušková M, Šedo O, Kšicová K, Zdráhal Z, Karpíšková R 2012: Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS. Int J Food Microbiol 159: 107-114 <>
14. EFSA 2008: Technical guidance prepared by the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) on the update of the criteria used in the assessment of bacterial resistance to antibiotics of human or veterinary importance. EFSA Journal 732: 1-15
15. EFSA 2012: EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP); Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA Journal 2012 10: e02740
16. EFSA 2018: Guidance on the characterization of microorganisms used as feed additives or as production organisms. EFSA Journal 2018 16: e05206
17. Egervärn M, Lindmark H, Olsson J, Roos S 2010: Transferability of a tetracycline resistance gene from probiotic Lactobacillus reuteri to bacteria in the gastrointestinal tract of humans. Anton Leeuw Int J G 97: 189-200 <>
18. Egervärn M, Roos S, Lindmark H 2009: Identification and characterization of antibiotic resistance genes in Lactobacillus reuteri and Lactobacillus plantarum. J Appl Microbiol 107: 1658-1668 <>
19. Ehrmann MA, Muller MRA, Vogel RF 2003: Molecular analysis of sourdough reveals Lactobacillus mindensis sp nov. Int J Syst Evol Micr 53: 7-13 <>
20. Feld L, Schjřrring S, Hammer K, Licht TR, Danielsen M, Krogfelt K, Wilcks A 2008: Selective pressure affects transfer and establishment of a Lactobacillus plantarum resistance plasmid in the gastrointestinal environment. J Antimicrob Chemoth 61: 845-852 <>
21. Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A, Danguleva, A, Ivanova G, Karapetkov N, Rumyan N, Karaivanova E 2015: Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotec Eq 29: 84-91 <>
22. Guo H, Pan L, Li L, Lu J, Kwok L, Menghe B, Zhang, H., Zhang W 2017: Characterization of antibiotic resistance genes from Lactobacillus isolated from traditional dairy products. J Food Sci 82: 724-730 <>
23. Hati S, Mandal S, Prajapati JB 2013: Novel starters for value added fermented dairy products. Curr Res Nutr Food Sci Jour 1: 83-91 <>
24. ISO10932/IDF223 guideline 2010: Milk and milk products: determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococal lactic acid bacteria (LAB). International Dairy Federation and International Organization for Standardization: 1-31
25. Jaimee G, Halami PM 2016: High level aminoglycoside resistance in Enterococcus, Pediococcus and Lactobacillus species from farm animals and commercial meat products. Ann Microbiol 66: 101-110 <>
26. Katla AK, Kruse H, Johnsen G, Herikstad H 2001: Antimicrobial susceptibility of starter culture bacteria used in Norwegian dairy products. Int J Food Microbiol 67: 147-152 <>
27. Leroy F, De Vuyst L 2004: Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci Tech 15: 67-78 <>
28. Li YM, Li LL, Kromann S, Chen MR, Shi L, Meng HC 2019: Antibiotic resistance of Lactobacillus spp. and Streptococcus thermophilus isolated from Chinese fermented milk products. Foodborne Pathog Dis 16: 221-228 <>
29. Ma Q, Fu Y, Sun H, Huang Y, Li L, Yu Q, Dinnyes A, Sun Q 2017: Antimicrobial resistance of Lactobacillus spp. from fermented foods and human gut. LWT-Food Sci Technol 86: 201-208 <>
30. Mathur S, Singh R 2005: Antibiotic resistance in food lactic acid bacteria – a review. Int J Food Microbiol 105: 281-295 <>
31. McArthur AG, Wright GD 2015: Bioinformatics of antimicrobial resistance in the age of molecular epidemiology. Curr Opin Microbiol 27: 45-50 <>
32. Nawaz M, Wang J, Zhou A, Ma C, Wu X, Moore JE, Millar BC, Xu J 2011: Characterization and transfer of antibiotic resistance in lactic acid bacteria from fermented food products. Curr Microbiol 62: 1081-1089 <>
33. Ortigosa M, Arizcun C, Irigoyen A, Oneca M, Torre P 2006: Effect of lactobacillus adjunct cultures on the microbiological and physicochemical characteristics of Roncal-type ewes’-milk cheese. Food Microbiol 23: 591-598 <>
34. Ouoba LII, Lei V, Jensen LB 2008: Resistance of potential probiotic lactic acid bacteria and bifidobacteria of African and European origin to antimicrobials: Determination and transferability of the resistance genes to other bacteria. Int J Food Microbiol 121: 217-224 <>
35. Pavlik I, Horvathova A, Dvorska L, Bartl J, Svastova P, du Maine R, Rychlik I 1999: Standardisation of restriction fragment length polymorphism analysis for Mycobacterium avium subspecies paratuberculosis. J Microbiol Meth 38: 155-167 <>
36. Ramirez MS, Tolmasky ME 2010: Aminoglycoside modifying enzymes. Drug Resist Update 13: 151-171 <>
37. Rossi F, Rizzotti L, Felis GE, Torriani S 2014: Horizontal gene transfer among microorganisms in food: Current knowledge and future perspectives. Food Microbiol 42: 232-243 <>
38. Shao Y, Zhang W, Guo H, Pan L, Zhang H, Sun T 2015: Comparative studies on antibiotic resistance in Lactobacillus casei and Lactobacillus plantarum. Food Control 50: 250-258 <>
39. Toh SM, Xiong L, Arias CA, Villegas MV, Lolans K, Quinn J, Mankin AS 2007: Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. Mol Microbiol 64: 1506-1514 <>
40. Toomey N, Bolton D, Fanning S 2010: Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs. Res Microbiol 161: 127-135 <>
41. Verraes C, Van Boxstael S, Van Meervenne E, Van Coillie E, Butaye P, Catry B, de Schaetzen MA, Van Huffel X, Imberechts H, Dierick K, Daube G, Saegerman C, De Block J, Dewulf J, Herman L 2013: Antimicrobial resistance in the food chain: a review. Int J Env Res Pub He 10: 2643-2669 <>
42. Wattam AR, Davis JJ, Assaf R, Boisvert S, Brettin T, Bun C, Conrad N, Dietrich EM, Disz T, Gabbard JL, Gerdes S, Henry CS, Kenyon RW, Machi D, Mao C, Nordberg EK, Olsen GJ, Murphy-Olson DE, Olson R, Overbeek R, Parrello B, Pusch GD, Shukla M, Vonstein V, Warren A, Xia F, Yoo H, Stevens RL 2017: Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center. Nucleic Acids Res 45: D535-D542 <>
43. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J 2017: Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Micr 67: 1613-1617 <>
44. Zago M, Fornasari ME, Carminati D, Burns P, Suŕrez V, Vinderola G, Reinheimer J, Giraffa G 2011: Characterization and probiotic potential of Lactobacillus plantarum strains isolated from cheeses. Food Microbiol 28: 1033-1040 <>
45. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, Aarestrup FM, Larsen MV 2012: Identification of acquired antimicrobial resistance genes. J Antimicrob Chemoth 67: 2640-2644 <>
46. Zhou N, Zhang JX, Fan MT, Wang J, Guo G, Wei XY 2012: Antibiotic resistance of lactic acid bacteria isolated from Chinese yogurts. J Dairy Sci 95: 4775-4783 <>
47. Zonenschain D, Rebecchi A, Morelli L 2009: Erythromycin- and tetracycline-resistant lactobacilli in Italian fermented dry sausages. J App Microbiol 107: 1559-1568 <>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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