Acta Vet. Brno 2020, 89: 391-400

Anti-ulcerogenic effect of osajin on indomethacin-induced gastric damage in rats

Huseyin Serkan Erol1, Ahmet Cakir2, Murat Koc3, Serkan Yildirim4, Mesut Halici5

1Kastamonu University, Faculty of Veterinary Medicine, Department of Biochemistry, Kastamonu, Turkey
2Kilis 7 Aralik University, Faculty of Science and Art, Department of Chemistry, Kilis, Turkey
3Ankara Yildirim Beyazit University, Public Health Institute, Department of Traditional, Complementary and Integrative Medicine, Ankara, Turkey
4Ataturk University, Faculty of Veterinary Medicine, Department of Pathology, Erzurum, Turkey
5Ataturk University, Faculty of Veterinary Medicine, Department of Biochemistry, Erzurum, Turkey

Received December 2, 2019
Accepted December 21, 2020

Ulcer is the most common undesirable result of using non-steroid anti-inflammatory drugs such as indomethacin. In the present study, osajin was experimentally used on indomethacin-induced gastric ulcer in rats. Osajin was purified from Maclura pomifera (Raf.) C. K. Schneid fruits by using the chromatographic methods. Thirty six rats were divided into six groups as follows: healthy (control), IND (indomethacin), RAN (ranitidine, 25 mg/kg), OSJ 100 (osajin, 100 mg/kg), OSJ 200 (200 mg/kg) and OSJ 400 (osajin, 400 mg/kg). Following a 24-h fasting, IND was administered to the treatment groups at a dose of 25 mg/kg. RAN and OSJ were given orally to rats following 5 min of IND administration. Then, gastric tissues were taken 6 h after the IND administration. Determination of the ulcer area, pathological evidence, and biochemical indices such as lipid peroxidation, superoxide dismutase, glutathione, and catalase were performed. IND generated diffuse ulcer areas, severe hyperaemia, oedema, necrotic epithelium, and mononuclear cell infiltration in the mucosa, and significantly increased lipid peroxidation while also decreasing the glutathione concentration, superoxide dismutase and catalase activities of the tissue. OSJ and RAN showed significant amelioration on ulcer area and biochemical indices. Therefore, OSJ may be potentially therapeutic for gastric ulcers.


This work was supported by the Scientific Funds of the Ataturk University (BAP-2012/364). We would like to thank Yunus Akturk for his assistance in preparing the histopathological part of the study.


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