The effect of a fibroblast growth factor, insulin-like growth factor, growth hormone, and Biolaminin 521 LN on the proliferative activity of cat stem cells

Mazurkevych, Anatoliy; Malyuk, Mykola; Melnyk, Oleg; Kovpak, Vitalii; Kovpak, Oksana; Kharkevych, Yuriy; Gryzinska, Magdalena; Jakubczak, Andrzej

doi:10.2754/avb202190010077

Acta Veterinaria > Archive > 2021, Vol. 90 > Issue 1 > The effect of a fibroblast growth…

Acta Vet. Brno 2021, 90: 77-85

https://doi.org/10.2754/avb202190010077

The effect of a fibroblast growth factor, insulin-like growth factor, growth hormone, and Biolaminin 521 LN on the proliferative activity of cat stem cells

Anatoliy Mazurkevych¹, Mykola Malyuk¹, Oleg Melnyk², Vitalii Kovpak³, Oksana Kovpak¹, Yuriy Kharkevych¹, Magdalena Gryzinska⁴, Andrzej Jakubczak⁴

¹National University of Life and Environmental Sciences of Ukraine, Department of Surgery and Pathophysiology named after acad. I.O. Povazhenko, Kyiv, Ukraine
²National University of Life and Environmental Sciences of Ukraine, Department of Animal Anatomy, Histology and Pathomorphology named after academician Vladimir G. Kas’janenko, Kyiv, Ukraine
³National University of Life and Environmental Sciences of Ukraine, Department of Obstetrics, Gynecology and Bio-techniques of Animal Reproduction, Kyiv, Ukraine
⁴University of Life Sciences in Lublin, Faculty of Animal Sciences and Bioeconomy, Institute of Biological Basis of Animal Production, Lublin, Poland

Received September 3, 2019
Accepted February 24, 2021

The wide use of cell technologies in clinical practice requires a large amount of cell material, which has led to improvement in culture conditions, making it possible to obtain more cell material in a shorter period of time. Thus, the purpose of our paper was to study the effects of different concentrations of an insulin-like growth factor (IGF-1), a fibroblast growth factor (FGF-2),| a growth hormone (rhGH), and Biolaminin 521 LN (LN 521) on the proliferative activity and genetic stability of stem cell cultures derived from the cat bone marrow, adipose tissue, and myocardium. Cell cultures for the experiment were obtained from the adipose tissue, bone marrow, and myocardium of a cat. Differences were found in the effects of the various growth promoters on the proliferative activity of cells in the culture. The IGF-1 demonstrated a positive effect on the proliferative activity of all cultures. The addition of the rhGH to the bone marrow-derived cell culture increased the size of the cells and decreased the proliferation index relative to the control group. The addition of the growth factors to the culture medium did not significantly increase the number of cells with altered karyotype in any of the cultures relative to the control group.