Acta Vet. Brno 2021, 90: 201-206

https://doi.org/10.2754/avb202190020201

The effect of L-glutamine and trehalose on dog sperm cryopreservation

Caner Öztürk1, Neşe Hayat Aksoy2

1Aksaray University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Aksaray, Turkey
2Aksaray University, Faculty of Veterinary Medicine, Department of Biochemistry, Aksaray, Turkey

Received December 15, 2020
Accepted May 26, 2021

References

1. Aboagla EME, Terada T 2003: Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod 69: 1245-1250 <https://doi.org/10.1095/biolreprod.103.017889>
2. Aisen E, Quintana M, Medina V, Morello H, Venturino A 2005: Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders. Cryobiology 50: 239-249 <https://doi.org/10.1016/j.cryobiol.2005.02.002>
3. Amidi F, Pazhohan A, Nashtaei MS, Khodarahmian M, Nekoonam S 2016: The role of antioxidants in sperm freezing: a review. Cell Tissue Bank 17: 745-756 <https://doi.org/10.1007/s10561-016-9566-5>
4. Amirat-Briand L, Bencharif D, Vera-Munoz O, Ali HBH, Destrumelle S, Desherces S, Schmidt E, Anton M, Tainturier D 2009: Effect of glutamine on post-thaw motility of bull spermatozoa after association with LDL (low density lipoproteins) extender: preliminary results. Theriogenology 71: 1209-1214 <https://doi.org/10.1016/j.theriogenology.2008.10.002>
5. Bakas LS, Disalvo EA 1991: Effect of Ca2+ on the cryoprotective action of trehalose. Cryobiology 28: 347-353 <https://doi.org/10.1016/0011-2240(91)90041-L>
6. Bucak MN, Ateşşahin A, Varışlı Ö, Yüce A, Tekin N, Akçay A 2007: The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after freeze–thawing process. Theriogenology 67: 1060-1067 <https://doi.org/10.1016/j.theriogenology.2006.12.004>
7. Bucak MN, Tuncer PB, Sarıözkan S, Ulutaş PA 2009: Comparison of the effects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities. Small Rumin Res 81: 13-17 <https://doi.org/10.1016/j.smallrumres.2008.10.003>
8. Büyükleblebici S, Tuncer PB, Bucak MN, Eken A, Sarıözkan S, Taşdemir U, Endirlik BÜ 2014: Cryopreservation of bull sperm: Effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results. Anim Reprod Sci 150: 77-83 <https://doi.org/10.1016/j.anireprosci.2014.09.006>
9. Caturla-Sánchez E, Sánchez-Calabuig MJ, Pérez-Gutiérrez JF, Cerdeira J, Castaño C, Santiago-Moreno J 2018: Vitrification of dog spermatozoa: Effects of two cryoprotectants (sucrose or trehalose) and two warming procedures. Cryobiology 80: 126-129 <https://doi.org/10.1016/j.cryobiol.2017.11.001>
10. Chhillar S, Singh VK, Kumar R, Atreja SK 2012: Effects of Taurine or Trehalose supplementation on functional competence of cryopreserved Karan Fries semen. Anim Reprod Sci 135: 1-7 <https://doi.org/10.1016/j.anireprosci.2012.08.029>
11. Chinoy NJ, Mehta D 1999: Beneficial effects of the amino acids glycine and glutamine on testis of mice treated with sodium fluoride. Fluoride 32: 162-170
12. Dawra V, Yadav B, Yadav S 2015: Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing. Vet World 8: 1027-1031 <https://doi.org/10.14202/vetworld.2015.1027-1031>
13. Erel O 2004: A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 37: 112-119 <https://doi.org/10.1016/j.clinbiochem.2003.10.014>
14. Erel O 2005: A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38: 1103-1111 <https://doi.org/10.1016/j.clinbiochem.2005.08.008>
15. Farstad W 2009: Cryopreservation of canine semen-new challenges. Reprod Domest Anim 44: 336-341 <https://doi.org/10.1111/j.1439-0531.2009.01418.x>
16. Gao D, Critser JK 2000: Mechanisms of cryoinjury in living cells. Ilar J 41: 187-196 <https://doi.org/10.1093/ilar.41.4.187>
17. Garner DL, Johnson LA 1995: Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53: 276–284 <https://doi.org/10.1095/biolreprod53.2.276>
18. Gobello C, Corrada YA 2003: Biotechnology in canine reproduction: an update. Analecta vet 23: 30-37
19. Gungor S, Ata A, Inanc ME 2018: Effects of trehalose and catalase on the viability and kinetic parameters of cryopreserved ram sperm. Acta Sci Vet 46: 1577
20. Holt WV 2000: Basic aspects of frozen storage of semen. Anim Reprod Sci 62: 3-22 <https://doi.org/10.1016/S0378-4320(00)00152-4>
21. Iqbal S, Andrabi SMH, Riaz A, Durrani AZ, Ahmad N 2016: Trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in Nili Ravi buffaloes (Bubalus bubalis). Theriogenology 85: 954-959 <https://doi.org/10.1016/j.theriogenology.2015.11.004>
22. Keskin N, Erdogan C, Bucak MN, Ozturk AE, Bodu M, Ili P, Baspinar N, Dursun S 2020: Cryopreservation effects on ram sperm ultrastructure. Biopreserv Biobank 18: 441-448 <https://doi.org/10.1089/bio.2020.0056>
23. Khlifaoui M, Battut I, Bruyas JF, Chatagnon G, Trimeche A, Tainturier D 2005: Effects of glutamine on post-thaw motility of stallion spermatozoa: an approach of the mechanism of action at spermatozoa level. Theriogenology 63: 138-149 <https://doi.org/10.1016/j.theriogenology.2004.04.012>
24. Kim SH, Yu DH, Kim YJ 2010: Effects of cryopreservation on phosphatidylserine translocation, intracellular hydrogen peroxide, and DNA integrity in canine sperm. Theriogenology 73: 282-292 <https://doi.org/10.1016/j.theriogenology.2009.09.011>
25. Kumar R, Atreja SK 2012: Effect of incorporation of additives in tris‐based egg yolk extender on buffalo (Bubalus bubalis) sperm tyrosine phosphorylation during cryopreservation. Reprod Domest Anim 47: 485-490 <https://doi.org/10.1111/j.1439-0531.2011.01908.x>
26. Lucio CF, Regazzi FM, Silva LCG, Angrimani DSR, Nichi M, Vannucchi CI 2016a: Oxidative stress at different stages of two-step semen cryopreservation procedures in dogs. Theriogenology 85: 1568-1575 <https://doi.org/10.1016/j.theriogenology.2016.01.016>
27. Lucio C, Silva LCG, Regazzi FM, Angrimani DSR, Nichi M, Assumpçăo MEO, Vannucchi CI 2016b: Effect of reduced glutathione (GSH) in canine sperm cryopreservation: In vitro and in vivo evaluation. Cryobiology 72: 135-140 <https://doi.org/10.1016/j.cryobiol.2016.02.001>
28. Nagy S, Jansen J, Topper EK, Gadella BM 2003: A triple-stain flow cytometric method to assess plasma and acrosome membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod 68: 1828-1835 <https://doi.org/10.1095/biolreprod.102.011445>
29. Neagu VR, García BM, Sandoval CS, Rodríguez AM, Ferrusola CO, Fernández LG, Tapia JA, Peńa FJ 2010: Freezing dog semen in presence of the antioxidant butylated hydroxytoluene improves post thaw sperm membrane integrity. Theriogenology 73: 645-650 <https://doi.org/10.1016/j.theriogenology.2009.10.021>
30. Park KS, Chun JL, Kim EY, Lee JH, Lee HS, Yamauchi N, Kyungbon L, Kim MK 2018: Protective effect of trehalose on canine spermatozoa in cryopreservation. J Fac Agric Kyushu Univ 63: 53-60 <https://doi.org/10.5109/1909902>
31. Quan GB, Hong QH, Lan ZG, Yang HY, Wu SS 2012: Comparison of the effect of various disaccharides on frozen goat spermatozoa. Biopreserv Biobank 10: 439-445 <https://doi.org/10.1089/bio.2012.0013>
32. Reddy NSS, Mohanarao GJ, Atreja SK 2010: Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim Reprod Sci 119: 183-190 <https://doi.org/10.1016/j.anireprosci.2010.01.012>
33. Renard P, Grizard G, Griveau JF, Sion B, Boucher D, Le Lannou D 1996: Improvement of motility and fertilization potential of post thaw human sperm using glutamine. Cryobiology 33: 311-319 <https://doi.org/10.1006/cryo.1996.0031>
34. Schafer S, Holzmann A 2000: The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci 59: 201-211 <https://doi.org/10.1016/S0378-4320(00)00073-7>
35. Setchell BP, Hinks NT, Voglmayr JK, Scott TW 1967: Amino acids in ram testicular fluid and semen and their metabolism by spermatozoa. Biochem J 105: 1061-1065 <https://doi.org/10.1042/bj1051061>
36. Topraggaleh TR, Shahverdi A, Rastegarnia A, Ebrahimi B, Shafiepour V, Sharbatoghli M, Esmaeili V, Janzamin E 2014: Effect of cysteine and glutamine added to extender on post‐thaw sperm functional parameters of buffalo bull. Andrologia 46: 777-783 <https://doi.org/10.1111/and.12148>
37. Tuncer PB, Taşdemir U, Büyükleblebici S, Özgürtaş T, Coşkun E, Erol H, Aydin FN, Gürcan İS 2013: Effects of different doses of trehalose supplementation in egg yolk extender in frozen–thawed Angora buck semen. Small Rumin Res 113: 383-389 <https://doi.org/10.1016/j.smallrumres.2013.04.012>
38. Wang S, Sun M, Wang N, Yang K, Guo H, Wang J, Zhang Y, Yue S, Zhou J 2018: Effects of L-glutamine on boar sperm quality during liquid storage at 17 °C. Anim Reprod Sci 191: 76-84 <https://doi.org/10.1016/j.anireprosci.2018.02.011>
39. Zhang M, Oldenhof H, Sieme H, Wolkers WF 2016: Freezing-induced uptake of trehalose into mammalian cells facilitates cryopreservation. Biochim Biophys Acta 1858: 1400-1409 <https://doi.org/10.1016/j.bbamem.2016.03.020>
40. Zhu Z, Fan X, Pan Y, Lu Y, Zeng W 2017: Trehalose improves rabbit sperm quality during cryopreservation. Cryobiology 75: 45-51 <https://doi.org/10.1016/j.cryobiol.2017.02.006>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Indexed in DOAJ

Archive