Acta Vet. Brno 2021, 90: 225-231

Comparison of cryoprotective methods for histological examination of rat and porcine lung tissue

Rea Jarošová1, Petra Ondráčková2, Zdeněk Patočka3, Zbyšek Sládek1

1Mendel University in Brno, Faculty of AgriSciences, Department of Morphology, Physiology and Animal Genetics, Brno, Czech Republic
2Veterinary Research Institute, Department of Immunology, Brno, Czech Republic
3Mendel University in Brno, Faculty of Forestry and Wood Technology, Department of Forest Management and Applied Geoinformatics, Brno, Czech Republic

Received August 13, 2020
Accepted May 26, 2021


1. Baatz JE, Newton DA, Riemer EC, Denlinger CE, Jones EE, Drake RR, Spyropoulos DD 2014: Cryopreservation of viable human lung tissue for versatile post-thaw analyses and culture. In Vivo 28: 411-423
2. Baijnath S, Shobo A, Bester LA, Singh SD, Kruger G, Naicker T, Govende T 2016: Small molecule distribution in rat lung: a comparison of various cryoprotectants as inflation media and their applicability to MSI. J Mol Histol 47: 213 <>
3. Bearer EL, Orci L 1986: A simple method for quick-freezing. J Electron Microsc Tech 3: 233-241 <>
4. Braber S,Verheijden KAT, Henricks PAJ, Kraneveld AD, Folkerts G 2010: A comparison of fixation methods on lung morphology in a murine model of emphysema. Am J Physiol Lung Cell Mol Physiol 299: L843-L851 <>
5. Churg AMD 1983: An inflation procedure for open lung biopsies. Am J Surg Pathol 7: 69-72 <>
6. Gatter KC, Falini B, Mason DY 1984: The use of monoclonal antibodies in histopathological diagnosis. Recent advances in histopathology. London, Churchill Livingstone, pp. 35-68
7. Gianoulis M, Chan N, Wright JL 1988: Inflation of lung biopsies for frozen section. Mod Pathol 1: 357-358
8. Halbower AC, Mason RJ, Abman SH, Tuder RM 1994: Agarose infiltration improves morphology of cryostat sections of lungs. Lab Invest 71: 149-153
9. Hausmann R, Bock H, Biermann T, Betz P 2004: Influence of lung fixation technique on the state of alveolar expansion – a histomorphometrical study. Leg Med (Tokyo) 6: 61-65 <>
10. Hosoda H, Shinohara N, Kojima N, Maruyama K, Handa Y, Uchida M, Koike H, Kobayashi Y 2001: Contrivance for frozen section diagnosis of early minute lung cancer during operation. Kyobu Geka 54: 913-916
11. Ling LH, Rogers SM, Tay V, Limmon GV, Dahai Z, Roger K 2009: Comparison of various tissue-preparation techniques for cryosectioning of frozen section mouse tissues. J Histotechnol 32: 186-189 <>
12. Myung JK, Choe G, Chung DH, Seo JW, Jheon S, Lee CT, Chung JH 2008: A simple inflation method for frozen section diagnosis of minute precancerous lesions of the lung. Lung Cancer 59: 198-202 <>
13. Pegg DE 2007: Principles of cryopreservation. Methods Mol Biol 368: 39-57 <>
14. Peters SR 2003: The art of embedding tissue for frozen section. Part I: A system for face down cryoembedding of tissues using freezing temperature embedding wells. J Histotechnol 26: 11-19 <>
15. Peters SR 2010: A practical guide to frozen section technique. Springer, New York, pp. 97-115
16. Prince AG, Porter DD 1975: Cryostat microtomy of lung tissue in an expanded state. Stain Technology 50: 43-45 <>
17. Rabinovitch M, Castaneda AR, Reid L 1981: Lung biopsy with frozen section as a diagnostic aid in patients with congenital heart defects. Am J Cardiol 47: 77-84 <>
18. Vacciana A, Ebeigbe AB, Hasse J, Swoboda L, Müller-Schweinitzer E 1994: Evidence for preservation of epithelial function in cryopreserved porcine and human bronchi. Exp Physiol 79: 409-414 <>
19. Von Ahlfen S, Missel A, Bendrat K, Schlumpberger M 2007: Determinants of RNA quality from FFPE samples. PLoS One 5;2: e1261 <>
20. Werner M, Chott A, Fabiano A, Battifora H 2000: Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol 24: 1016-1019 <>
21. Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW 2002: Diseases of Swine. 10th edn, Iowa: Wiley, Ames, 685, 749 p.
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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