Acta Vet. Brno 2021, 90: 271-276

https://doi.org/10.2754/avb202190030271

Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

Miroslava Mráčková, Lenka Vojtěchovská, Petra Andrlová, Šárka Krisová, Markéta Sedlinská

University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Equine Clinic, Brno, Czech Republic

Received December 17, 2020
Accepted August 31, 2021

Two substances at two concentrations each were added to frozen-thawed stallion epididymal spermatozoa to improve motility indicators, which were assessed 30, 60, 120, 180 and 240 min after thawing. Pentoxifylline was added at concentrations of 3.6 mM (1 mg/ml) and 7.18 mM (2 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa throughout the study. The other substance, caffeine, was added at concentrations of 2 mM (0.4 mg/ml) and 5.5 mM (1 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa only 30 and 60 min after thawing. Subsequently, 180 min after thawing, improvement was found only in total sperm motility.

Funding

The authors are grateful to ITA VFU Brno for the financial support of this research (FVL – Illek/ITA 2020).

References

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