Acta Vet. Brno 2021, 90: 277-286

Reproductive toxicity of heavy metals in fallow deer in vitro

Ehdaa Eltayeb Eltigani Abdelsalam, Hana Banďouchová, Tomáš Heger, Miroslava Kaňová, Kateřina Kobelková, Monika Němcová, Vladimír Piaček, Jana Sedláčková, Veronika Seidlová, František Vitula, Jiří Pikula

University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Ecology and Diseases of Zoo Animals, Game, Fish, and Bees, Brno, Czech Republic

Received October 21, 2020
Accepted August 31, 2021


1. Abdelsalam EEE, Bandouchova H, Heger T, Kanova M, Kobelkova K, Nemcova M, Piacek V, Sedlackova J, Seidlova V, Vitula F, Pikula J 2020: Polychlorinated biphenyl toxicity in the thyroid gland of wild ungulates: an in vitro model. Acta Vet Brno 89: 151-162 <>
2. Apostoli P, Telišman S, Sager PR 2007: Reproductive and developmental toxicity of metals. In: Nordberg GF, Fowler BA, Nordberg M, Friberg LT (Eds): Handbook on the Toxicology of Metals. Third edn, Academic Press, Burlington, MA, USA, 975 p.
3. Ariza ME, Bijur GN, Williams MV 1999: Environmental Metal Pollutants: Sources and Determinants of Toxicity. In: Ariza ME, Bijur GN, Williams MV (Eds): Enivronmental Metal Pollutants, Reactive Oxygen Intermediaries and Genotoxicity. Springer, Boston, MA, USA, 189 p.
4. Bertin G, Averbeck D 2006: Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review). Biochimie 88: 1549-1559 <>
5. Carocci A, Rovito N, Sinicropi MS, Genchi G 2014: Mercury Toxicity and Neurodegenerative Effects. In: Whitacre DM (Ed.): Reviews of Environmental Contamination and Toxicology. Springer International Publishing, Cham, Germany, pp. 1-18
6. Čelechovská O, Malota L, Zima S 2008: Entry of heavy metals into food chains: A 20-year comparison study in Northern Moravia (Czech Republic). Acta Vet Brno 77: 645-652 <>
7. Crespo-López ME, Macędo GL, Pereira SID, Arrifano GPF, Picanço-Diniz DLW, do Nascimento JLM, Herculano AM 2009: Mercury and human genotoxicity: Critical considerations and possible molecular mechanisms. Pharmacol Res 60: 212-220 <>
8. Cupertino MC, Novaes RD, Santos EC, Neves AC, Silva E, Oliveira JA, Matta SLP 2017: Differential susceptibility of germ and Leydig cells to cadmium-mediated toxicity: Impact on testis structure, adiponectin levels, and steroidogenesis. Oxid Med Cell Longev 2017: 1-11 <>
9. Damkova V, Sedlackova J, Bandouchova H, Peckova L, Vitula F, Hilscherova K, Paskova V, Kohoutek J, Pohanka M, Pikula J 2009: Effects of cyanobacterial biomass on avian reproduction: a Japanese quail model. Neuroendocrinol Lett 30: 205-210
10. Damkova V, Paskova V, Sedlackova J, Bandouchova H, Hilscherova K, Novotny L, Peckova L, Vitula F, Pohanka M, Pikula J 2011: Testicular toxicity of cyanobacterial biomass in Japanese quails. Harmful Algae 10: 612-618 <>
11. Flieger M, Bandouchova H, Cerny J, Chudíčková M, Kolarik M, Kovacova V, Martínková N, Novák P, Šebesta O, Stodůlková E, Pikula J 2016: Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection. Sci Rep 6: 33200 <>
12. Florea AM, Taban J, Varghese E, Alost BT, Moreno S, Büsselberg D 2013: Lead (Pb2+) neurotoxicity: Ion-mimicry with calcium (Ca2+) impairs synaptic transmission. A review with animated illustrations of the pre- and post-synaptic effects of lead. Glob J Health Sci 2013: 4
13. Fowler BA 1998: Roles of lead-binding proteins in mediating lead bioavailability. Environ Health Persp 106: 1585-1587 <>
14. Garcia MHDM, Moreno DH, Rodriguez FS, Beceiro L, Fidalgo ALE, Lopez MP 2011: Sex- and age-dependent accumulation of heavy metals (Cd, Pb and Zn) in liver, kidney and muscle of roe deer (Capreolus capreolus) from NW Spain. J Environ Sci Health A 46: 109-116 <>
15. Girotti AW 1998: Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 39: 1529-1542 <>
16. Gyori BM, Venkatachalam G, Thiagarajan PS, Hsu D, Clement MV 2014: OpenComet: an automated tool for comet assay image analysis. Redox Biol 2: 457-465 <>
17. Hamilton RP, Seamark RF 1978: Lipid content of sheep ovarian follicles in culture. Aust J Biol Sci 31: 621-628 <>
18. Hruba H, Abdelsalam EEE, Anisimov N, Bandouchova H, Havelkova B, Heger T, Kanova M, Kovacova V, Nemcova M, Piacek V, Sedlackova J, Vitula F, Pikula J 2019: Reproductive toxicity of fluoroquinolones in birds. BMC Vet Res 15: 209 <>
19. Jia Y, Lin J, Mi Y, Zhang C 2011: Quercetin attenuates cadmium-induced oxidative damage and apoptosis in granulosa cells from chicken ovarian follicles. Reprod Toxicol 31: 477-485 <>
20. Kala S, Kaushik R, Singh KP, Kadam PH, Singh MK, Manik RS, Singla SK, Palta P, Chauhan MS 2012: In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell. J Assist Reprod Gen 29: 1335-1342 <>
21. Kovacova V, Abdelsalam EEE, Bandouchova H, Brichta J, Havelkova B, Piacek V, Vitula F, Pikula J 2016: Cytotoxicity of ketamine, xylazine and Hellabrunn mixture in liver-, heart- and kidney-derived cells from fallow deer. Neuroendocrinol Lett 37 (Suppl. 1): 78-83
22. Kuiters AT 1996: Accumulation of cadmium and lead in red deer and wild boar at the Veluwe, The Netherlands. Vet Quart 18 (Suppl. 3): 134-135 <>
23. Lazarus M, Orct T, Blanuša M, Vicković I, Šoštarić B 2008: Toxic and essential metal concentrations in four tissues of red deer (Cervus elaphus) from Baranja, Croatia. Food Addit Contam A 25: 270-283 <>
24. Magoffin DA 2005: Ovarian theca cell. Int J Biochem Cell Biol 37: 1344-1349 <>
25. Morris P 1972: A review of mammalian age determination methods. Mammal Rev 2: 69-104 <>
26. Penna S, Pocino M, Marval MJ, Lloreta J, Gallardo L, Vila J 2009: Modifications in rat testicular morphology and increases in IFN-γ serum levels by the oral administration of subtoxic doses of mercuric chloride. Syst Biol Reprod Med 55: 69-84 <>
27. Piasek M, Laskey JW 1999: Effects of in vitro cadmium exposure on ovarian steroidogenesis in rats. J Appl Toxicol 19: 211-217 <<211::AID-JAT568>3.0.CO;2-4>
28. Paskova V, Paskerova H, Pikula J, Bandouchova H, Sedlackova J, Hilscherova K 2011: Combined exposure of Japanese quails to cyanotoxins, Newcastle virus and lead: Oxidative stress responses. Ecotox Environ Safe 74: 2082-2090 <>
29. Pikula J, Hajkova P, Bandouchova H, Bednarova I, Adam V, Beklova M, Kral J, Ondracek K, Osickova J, Pohanka M, Sedlackova J, Skochova H, Sobotka J, Treml F, Kizek R 2013: Lead toxicosis of captive vultures: case description and responses to chelation therapy. BMC Vet Res 9: 11 <>
30. Pokorny B 2000: Roe deer Capreolus capreolus as an accumulative bioindicator of heavy metals in Slovenia. Web Ecol 1: 54-62 <>
31. Singerman A 2000: Exposure to Toxic Metals: Biological Effects and Their Monitoring. In: Vercruysse A (Ed.): Hazardous Metals in Human Toxicology. First edn, Elsevier Science Publisher, Amsterdam, Netherlands, 334 p.
32. Siu ER, Mruk DD, Porto CS, Cheng CY 2010: Cadmium-induced testicular injury. Toxicol Appl Pharm 238: 240-249 <>
33. Stadnicka A 1980: Localization of mercury in the rat ovary after oral administration of mercuric chloride. Acta Histochem 67: 227-233 <>
34. Svoboda M, Drápal J, Haruštiaková D, Svobodová Z 2020: A multiannual survey of cadmium content in pig tissues collected in the Czech Republic during the years 2015–2019. Acta Vet Brno 89: 349-355 <>
35. Swim HE, Parker RF 1958: The role of carbon dioxide as an essential nutrient for six permanent strains of fibroblasts. J Biophys Biochem Cytol 4: 525-528 <>
36. Taupeau C, Poupon J, Nomé F, Lefèvre B 2001: Lead accumulation in the mouse ovary after treatment-induced follicular atresia. Reprod Toxicol 15: 385-391 <>
37. Wadi SA, Ahmad G 1999: Effects of lead on the male reproductive system in mice. J Toxicol Environ Health Part A 56: 513-521
38. Wang Y, Wang X, Wang Y, Fan R, Qiu C, Zhong S, Wei L, Luo D 2015: Effect of cadmium on cellular ultrastructure in mouse ovary. Ultrastruct Pathol 39: 324-328 <>
39. Wani AL, Ara A, Usmani JA 2015: Lead toxicity: A review. Interdiscip Toxicol 8: 55-64 <>
40. Wolfe MF, Schwarzbach S, Sulaiman RA 1998: Effects of mercury on wildlife: a comprehensive review. Environ Toxicol Chem 17: 146-160 <>
41. Yang JM, Arnush M, Chen QY, Wu XD, Pang B, Jiang XZ 2003: Cadmium-induced damage to primary cultures of rat Leydig cells. Reprod Toxicol 17: 553-560 <>
42. Yoo HY, Chang MS, Rho HM 1999: Heavy metal-mediated activation of the rat Cu/Zn superoxide dismutase gene via a metal-responsive element. Mol Gen Genet 262: 310-313 <>
43. Zhang M, He Z, Wen L, Wu J, Yuan L, Lu Y, Guo C, Zhu L, Deng S, Yuan H 2010: Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure. Reprod Biol Endocrinol 8: 97 <>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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