Acta Vet. Brno 2022, 91: 51-58

Translocation stress is reflected in corticosterone metabolites in pheasant (Phasianus colchicus) droppings

Martina Volfová1, Rupert Palme2, Zuzana Machovcová1, Eva Voslářová1, Gabriela Lukešová1, Vladimír Večerek1

1University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Protection and Welfare & Veterinary Public Health, Brno, Czech Republic
2University of Veterinary Medicine Vienna, Department of Biomedical Sciences, Austria

Received October 20, 2021
Accepted November 23, 2021


1. Balcombe JP, Barnard ND, Sandusky C 2004: Laboratory routines cause animal stress. J Am Assoc Lab Anim Sci 43: 42-51
2. Baltic M, Jenni-Eiermann S, Arlettaz R, Palme R 2005: A noninvasive technique to evaluate human-generated stress in the black grouse. Ann NY Acad Sci 1046: 81-95 <>
3. Bedanova I, Pistekova V, Forejtek P, Chloupek J, Voslarova E, Plhalova L, Vecerek V 2018: Short-time effect of stress associated with transport of juvenile pheasants (Phasianus colchicus) as affected by age. Berl Münch Tierärztl Wschr 131: 25-30
4. Busso J, Dominchin MF, Marin RH, Palme R 2013: Cloacal gland, endocrine testicular, and adrenocortical photoresponsiveness in male Japanese quail exposed to short days. Dom Anim Endocrinol 44: 151-156 <>
5. Carenzi C, Verga M 2009: Animal welfare: review of the scientific concept and definition. Ital J Anim Sci 8: 21-30 <>
6. Chloupek P, Voslarova E, Suchy Jr. P, Bedanova I, Pistekova V, Vitula F, Chkoupek J, Vecerek V 2009: Influence of pre-sampling handling duration on selected biochemical indices in the common pheasant (Phasianus colchicus). Acta Vet Brno 78: 23-28 <>
7. Dehnhard M, Schreer A, Krone O, Jewgenow K, Krause M, Grossmann R 2003: Measurement of plasma corticosterone and fecal glucocorticoid metabolites in the chicken (Gallus domesticus), the great cormorant (Phalacrocorax carbo), and the goshawk (Accipiter gentilis). Gen Comp Endocrinol 131: 111-120 <>
8. Draycott RAH, Woodburn MIA, Carroll JP, Sage RB 2005: Effects of spring supplementary feeding on population density and breeding success of released pheasants Phasianus colchicus in Britain. Wildlife Biol 11: 117-182 <[177:EOSSFO]2.0.CO;2>
9. Goymann W 2005: Noninvasive monitoring of hormones in bird droppings: Physiological validation, sampling, extraction, sex differences, and the influence of diet on hormone metabolite levels. Ann NY Acad Sci 1046: 35-53 <>
10. Legagneux P, Gauthier G, Chastel O, Picard G, Bęty J 2011: Do glucocorticoids in droppings reflect baseline level in birds captured in the wild? A case study in snow geese. Gen Comp Endocrinol 172: 440-445 <>
11. Malikova M, Voslarova E, Vecerek V 2018: Non-invasive faecal monitoring of stress in captive and free-ranging wild animals. Berl Münch Tierärztl Wschr 131: 150-158
12. Manteca X, Salas M 2015: Concept of animal welfare. ZAWEC 1: 1-2
13. Mitchell MA, Kettlewell PJ 2009: Welfare of poultry during transport – a review. Proceedings of the 8th European Symposium on Poultry Welfare, Italy
14. Morgan KN, Tromborg CHT 2007: Sources of stress in captivity. Appl Anim Behav Sci 102: 262-302 <>
15. Möstl E, Palme R 2002: Hormones as indicators of stress. Domest Anim Endocrinol 23: 67-74 <>
16. Möstl E, Rettenbacher S, Palme R 2005: Measurement of corticosterone metabolites in birds’droppings: An analytical approach. Ann NY Acad Sci 1046: 17-34 <>
17. Palme R 2019: Non-invasive measurement of glucocorticoids: Advances and problems. Physiol Behav 199: 229-243 <>
18. Palme R, Rettenbacher S, Touma C, El-Bahr SM, Möstl E 2005: Stress hormones in mammals and birds: Comparative aspects regarding metabolism, excretion, and noninvasive measurement in fecal samples. Ann NY Acad Sci 1040: 162-171 <>
19. Palme R, Touma C, Arias N, Dominchin MF, Lepschy M 2013: Steroid extraction: Get the best out of faecal samples. Wiener Tierärztl Mschrift – Vet Med Austria 100: 238-246
20. Renčínová V, Voslářová E, Bedáňová I, Večerek V 2021: Pest flies on dairy farms affect behaviour and welfare of dairy cows during summer season. Acta Vet Brno 90: 255-262 <>
21. Rettenbacher S, Möstl E, Hackl R, Ghareeb K, Palme R 2004: Measurement of corticosterone metabolites in chicken droppings. Br Poult Sci 45: 704-711 <>
22. Rettenbacher S, Palme R 2009: Biological validation of a non-invasive method for stress assessment in chickens. Berl Münch Tierärztl Wschr 122: 8-12
23. Thiel D, Jenni-Eiermann S, Palme R 2005: Measuring corticosterone metabolites in droppings of capercaillies (Tetrao urogallus). Ann NY Acad Sci 1046: 96-108 <>
24. Touma C, Palme R 2005: Measuring fecal glucocorticoid metabolites in mammals and birds: the importance of validation. Ann N Y Acad Sci 1046: 54-74 <>
25. Večerek V, Voslářová E, Semerád Z 2020: Patho-anatomic findings in finisher pigs, sows and piglets detected during veterinary slaughterhouse inspection. Acta Vet Brno 89: 341-347 <>
26. Voslarova E, Bedanova I, Chloupek P, Pistekova V, Chloupek J, Vecerek V 2012: Changes in selected biochemical indices in common pheasants in response to their transport. Folia Vet 56: 58-59
27. Voslarova E, Bedanova I, Vecerek V, Pistekova V, Chloupek P, Suchy P 2006: Changes in haematological profile of common pheasant (Phasianus colchicus) induced by transit to pheasantry. Dtsch Tierärztl Wochenschr 113: 361-400
28. Washburn BE, Millspaugh JJ, Schulz JH, Jones SB, Mong T 2003: Using fecal glucocorticoids for stress assessment in mourning doves. Condor 105: 696-706 <>
29. Yates JW, Main DCJ 2005: Assessment of positive welfare: A review. Vet J 175: 293-300 <>
30. Yalçin S, Özkan S, Oktay G, Cabuk M, Erbayraktar Z, Bilgili SF 2004: Age-related effects of catching, crating, and transportation at different seasons on core body temperature and physiological blood parameters in broilers. J Appl Poult Res 13: 549-560 <>
31. Zar JH 1999: Biostatistical Analysis. 4th edn. Prentice-Hall, Inc., New Jersey, 663 p.
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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