Acta Vet. Brno 2022, 91: 69-75

Does blood sampling from caudal vessels in fish produce parameter values different from those obtained by heart puncture?

Ivana Mikulíková1, Miroslava Palíková1,2, Ivana Papežíková1,2, Jana Blahová3, Ivona Toulová1, Hana Minářová1,4

1University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Brno, Czech Republic
2Mendel University in Brno, Faculty of Agronomy, Department of Zoology, Fisheries, Hydrobiology and Apiculture, Brno, Czech Republic
3University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Protection and Welfare and Veterinary Public Health, Brno, Czech Republic
4Veterinary Research Institute, Department of Infectious Diseases and Preventive Medicine, Brno, Czech Republic

Received August 2, 2021
Accepted October 12, 2021

Analyses of blood samples in ichthyology are of importance for assessment of fish health as well as fish responses to environmental stressors. The measurement results may be affected by multiple factors. This study aimed at assessment of the influence of the blood collection site by comparing dual values of indices measured in samples obtained both from the heart and puncturing caudal vessels in the same fish specimens. Our results revealed that the sampling site did not significantly influence measured variables including haematological indices, the plasma biochemistry profile, acid-base balance parameters and the phagocytic activity. To conclude, for the rainbow trout (Oncorhynchus mykiss) both sampling methods are interchangeable with regard to the above-mentioned indices.


The study was supported by FVHE/Pikula/ITA2021.


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