Acta Vet. Brno 2022, 91: 125-131

The effect of supplementation of follicle-stimulating hormone (FSH) into the Ovsynch protocol to increase the pregnancy rate in cyclic lactating dairy cows

Gulnaz Yilmazbas-Mecitoglu1, Abdulkadir Keskin1, Ebru Karakaya-Bilen2, Umut Taşdemir3, Ahmet Gumen1

1University of Bursa Uludag, Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Bursa, Turkey
2University of Siirt, Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, Siirt, Turkey
3Aksaray University, Faculty of Veterinary Medicine, Department of Artificial Insemination, Aksaray, Turkey

Received September 23, 2021
Accepted February 1, 2022


1. Adams GP, Jaiswal R, Singh J, Malhi P 2008: Progress in understanding ovarian follicular dynamics in cattle. Theriogenology 69: 72-80 <>
2. Butler WR 2000: Nutritional interactions with reproductive performance in dairy cattle. Anim Reprod Sci 60-61: 449-457 <>
3. Ferguson JD, Galligan DT, Thomsen N 1994: Principal descriptors of body condition score in Holstein cows. J Dairy Sci 77: 2695-2703 <>
4. Fricke PM, Wiltbank MC 1999: Effect of milk production on the incidence of double ovulation in dairy cows. Theriogenology 52: 1133-1143 <>
5. Galvăo KN, Santos JEP 2010: Factors affecting synchronization and conception rate after the Ovsynch protocol in lactating Holstein cows. Reprod Domest Anim 45: 439-446 <>
6. Kenyon AG, Lopes G Jr, Mendonça LG, Lima JR, Bruno RG, Denicol AC, Chebel RC 2012: Ovarian responses and embryo survival in recipient lactating Holstein cows treated with equine chorionic gonadotropin. Theriogenology 77: 400-411 <>
7. Keskin A, Mecitoğlu G, Bilen E; Güner B; Orman A, Okut H, Gumen A 2016: The effect of ovulatory follicle size at the time of insemination onpregnancy rate in lactating dairy cows. Turk J Veter Anim Sci 40: 68-74 <>
8. Keskin A, Yilmazbas-Mecitoglu G, Gumen A, Karakaya E, Darici R, Okut H 2010: Effect of hCG vs. GnRH at the beginning of the Ovsynch on first ovulation and conception rates in cyclic lactating dairy cows. Theriogenology 74: 602-607 <>
9. Lopez H, Sartori R, Wiltbank MC 2005: Reproductive hormones and follicular growth during development of one or multiple dominant follicles. Biol Reprod 72: 788-795 <>
10. Lynch CO, Kenny DA, Childs S, Diskin MG 2010: The relationship between ovulatory endocrine and follicular activity on corpus luteum size, function, and subsequent embryo survival. Theriogenology 73: 90-198 <>
11. Mihm M, Austin EJ 2002: The final stages of dominant follicle selection in cattle. Domest Anim Endocrinol 23: 155-166 <>
12. Mihm M, Diskin MG, Roche JF 1996: Regulation of follicle wave growth in cattle. Reprod Dom Anim 31: 531-538
13. Navanukraw C, Redmer DA, Reynolds LP, Kirsch JD, Grazul-Bilska AT, Fricke PM 2004: A modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. J Dairy Sci 87: 1551-1557 <>
14. Pursley JR, Kosorok MR, Wiltbank MC 1997: Reproductive management of lactating dairy cows using synchronization of ovulation. J Dairy Sci 80: 301-306 <>
15. Revah I, Butler WR 1996: Prolonged dominance of follicles and reduced viability of bovine oocytes. J Reprod Fertil 106: 39-47 <>
16. Sellars CB, Dalton JC, Manzo R, Day J, Ahmadzadeh A 2006: Time and incidence of ovulation and conception rates after incorporating estradiol cypionate into a timed artificial insemination protocol. J Dairy Sci 89: 620-626 <>
17. Simőes RAL, Satrapa RA, Rosa FS, Piagentini M, Castilho ACS, Erena RL, Trinca LA, Nogueira MFG, Burantini J Jr, Barros CM 2012: Ovulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cows. Theriogenology 77:139-147 <>
18. Sirard MA, Desrosier S, Assidi M 2007: In vivo and in vitro effects of FSH on oocyte maturation and developmental competence. Theriogenology 68: 71-76 <>
19. Souza AH, Gumen A, Silva EPB, Cunha AP, Guenther JN, Peto CM 2007: Supplementation with estradiol-17b before the last GnRH of the Ovsynch protocol in lactating dairy cows. J Dairy Sci 90: 4623-4634 <>
20. Souza AH, Viechneski S, Lima FA, Silva FF, Araújo R, Bó GA, Wiltbank MC, Baruselli PS 2009: Effects of equine chorionic gonadotropin and type of ovulatory stimulus in timed-AI protocol on reproductive responses in dairy cow. Theriogenology 72: 10-21 <>
21. Thatcher WW, Moreira F, Santos JEP 2000: Strategies to improve reproductive management of dairy cows. Adv Dairy Technol 12:177-193
22. Vasconcelos JLM, Silcox RW, Rosa GJ, Pursley JR, Wiltbank MC 1999: Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology 52: 1067-1078 <>
23. Wiltbank MC, Lopez H, Sartori R, Sangsritavong S, Gumen A 2006: Changes in reproductive physiology of lactating dairy cows due to elevated steroid metabolism. Theriogenology 65: 17-29 <>
24. Wiltbank MC, Fricke PM, Sangritasvong S, Sartori R, Ginther OJ 2000: Mechanisms that prevent and produce double ovulations in dairy cattle. J Dairy Sci 83: 2998-3007 <>
25. Yilmazbas-Mecitoglu G, Karakaya E, Keskin A, Alkan A, Gumen A 2013: Reducing the duration between gonadotropin-releasing hormone (GnRH) and prostaglandin F2α treatment in the Ovsynch protocol to 6 days improved ovulation to second GnRH treatment, but inclined to reduce fertility. J Dairy Sci 96: 3817-3824 <>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Indexed in DOAJ