Acta Vet. Brno 2022, 91: 375-381

Effect of spiramycin and metronidazole on canine dental biofilm bacteria

Jana Kačírová1, Miriam Sondorová1, Natália Šurín Hudáková1, Tomáš Lipták2, Aladár Maďari2, Kristína Mravcová3, Lívia Kolesár Fecskeová4, Rastislav Mucha5, Marián Maďar1

1University of Veterinary Medicine and Pharmacy in Košice, Department of Microbiology and Immunology, Košice, Slovakia
2University of Veterinary Medicine and Pharmacy in Košice, University Veterinary Hospital, Košice, Slovakia
3Czech Academy of Sciences, v.v.i, Institute of Vertebrate Biology, Brno, Czech Republic
4P.J. Šafárik University in Košice, Faculty of Medicine, Associated Tissue Bank, Košice, Slovakia
5Biomedical Research Center of the Slovak Academy of Sciences, Institute of Neurobiology, Košice, Slovakia

Received July 15, 2022
Accepted October 10, 2022


1. Abranches J, Zeng L, Kajfasz JK, Palmer SR, Chakraborty B, Wen ZT, Richards VP, Brady LJ, Lemos JA 2018: Biology of oral streptococci. Microbiol Spectr 6: 1-18 <>
2. Albuquerque C, Morinha F, Requicha J, Martins T, Dias I, Guedes-Pinto H, Bastos E, Viegas C 2012: Canine periodontitis: the dog as an important model for periodontal studies. Vet J 191: 299-305 <>
3. Bell SE, Nash AK, Zanghi BM, Otto CM, Perry EB 2020: An assessment of the stability of the canine oral microbiota after probiotic administration in healthy dogs over time. Front Vet Sci 7: 616 <>
4. Calcagnile M, Bettini S, Damiano F, Talŕ A, Tredici SM, Pagano R, Di Salvo M, Siculella L, Fico D, De Benedetto GE, Valli L, Alifano P 2018: Stimulatory effects of methyl-β-cyclodextrin on spiramycin production and physical-chemical characterization of nonhost@guest complexes. ACS Omega 3: 2470-2478 <>
5. Carvalho CM, Rahal SC, Dos Reis Mesquita L, Castilho MS, Kano WT, Mamprim MJ 2015: Mandibulectomy for treatment of fractures associated with severe periodontal disease. Can Vet J 56: 292-294
6. Chiappe V, Gómez M, Fernández-Canigia L, Romanelli H 2011: The effect of spiramycin on Porphyromonas gingivalis and other “classic” periopathogens. Acta Odontol Latinoam 24: 115-121
7. Dewhirst FE, Klein EA, Thompson EC, Blanton JM, Chen T, Milella L, Buckley CM, Davis IJ, Bennett ML, Marshall-Jones ZV 2012: The canine oral microbiome. PloS One 7: e36067 <>
8. Di Bello A, Buonavoglia A, Franchini D, Valastro C, Ventrella G, Greco MF, Corrente M 2014: Periodontal disease associated with red complex bacteria in dogs. J Small Anim Pract 55: 160-163 <>
9. Elliott DR, Wilson M, Buckley CM, Spratt DA 2005: Cultivable oral microbiota of domestic dogs. J Clin Microbiol 43: 5470-5476 <>
10. Giboin H, Becskei C, Civil J, Stegemann MR 2012: Safety and efficacy of cefovecin (Convenia®) as an adjunctive treatment of periodontal disease in dogs. Open J Vet Med 2: 89-97 <>
11. Howard KC, Gonzalez OA, Garneau-Tsodikova S 2021: Porphyromonas gingivalis: where do we stand in our battle against this oral pathogen? RSC Med Chem 12: 666-704 <>
12. Kapoor A, Malhotra R, Grover V, Grover D 2012: Systemic antibiotic therapy in periodontics. Dent Res J (Isfahan) 9: 505-515 <>
13. Klinge B, Kuvatanasuhati J, Attström R, Kalfas S, Edwardsson S 1992: The effect of topical metronidazole therapy on experimentally-induced periodontitis in the beagle dog. J Clin Periodontol 19: 702-707 <>
14. Löfmark S, Edlund C, Nord CE 2010: Metronidazole is still the drug of choice for treatment of anaerobic infections. Clin Infect Dis 50: 16-23 <>
15. Nomura R, Inaba H, Yasuda H, Shirai M, Kato Y, Murakami M, Iwashita N, Shirahata S, Yoshida S, Matayoshi S, Yasuda J, Arai N, Asai F, Matsumoto-Nakano M, Nakano K 2020: Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha. Sci Rep 10: 3113 <>
16. Poulet PP, Duffaut D, Barthet P, Brumpt I 2005: Concentrations and in vivo antibacterial activity of spiramycin and metronidazole in patients with periodontitis treated with high-dose metronidazole and the spiramycin/metronidazole combination. J Antimicrob Chemother 55: 347-351 <>
17. Rams TE, Dujardin S, Sautter JD, Degener JE, van Winkelhoff AJ 2011: Spiramycin resistance in human periodontitis microbiota. Anaerobe 17: 201-205 <>
18. Rodrigues MX, Fiani N, Bicalho RC, Peralta S 2021: Preliminary functional analysis of the subgingival microbiota of cats with periodontitis and feline chronic gingivostomatitis. Sci Rep 11: 6896 <>
19. Santibáñez R, Rodríguez-Salas C, Flores-Yáñez C, Garrido D, Thomson P 2021: Assessment of changes in the oral microbiome that occur in dogs with periodontal disease. Vet Sci 8: 291 <>
20. Senhorinho GN, Nakano V, Liu C, Song Y, Finegold SM, Avila-Campos MJ 2012: Occurrence and antimicrobial susceptibility of Porphyromonas spp. and Fusobacterium spp. in dogs with and without periodontitis. Anaerobe 18: 381-385 <>
21. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr 1998: Microbial complexes in subgingival plaque. J Clin Periodontol 25: 134-144 <>
22. Stephan B, Greife HA, Pridmore A, Silley P 2008: Activity of pradofloxacin against Porphyromonas and Prevotella spp. implicated in periodontal disease in dogs: susceptibility test data from a European multicenter study. Antimicrob Agents Chemother 52: 2149-2155 <>
23. Suzuki M, Kimura M, Imaoka K, Yamada A 2010: Prevalence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in dogs and cats determined by using a newly established species-specific PCR. Vet Microbiol 144: 172-176 <>
24. Takada K, Hirasawa M 2000: Expression of trypsin-like activity by the genera Corynebacterium and Actinomyces in canine periodontitis. J Med Microbiol 49: 621-625 <>
25. Vesty A, Biswas K, Taylor MW, Gear K, Douglas RG 2017: Evaluating the impact of DNA extraction method on the representation of human oral bacterial and fungal communities. PLoS One 12: e0169877 <>
26. Větrovský T, Baldrian P, Morais D 2018: SEED 2: a user-friendly platform for amplicon high-throughput sequencing data analyses. Bioinformatics 34: 2292-2294 <>
27. Yoshida S, Inaba H, Nomura R, Murakami M, Yasuda H, Nakano K, Matsumoto-Nakano M 2021: Efficacy of FimA antibody and clindamycin in silkworm larvae stimulated with Porphyromonas gulae. J Oral Microbiol 13: 1914499 <>
28. Zajkowska J, Król M, Falkowski D, Syed N, Kamieńska A 2016: Capnocytophaga canimorsus – an underestimated danger after dog or cat bite – review of literature. Przegl Epidemiol 70: 289-295
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Indexed in DOAJ