Acta Vet. Brno 2023, 92: 323-328

https://doi.org/10.2754/avb202392040323

Determination of the mycobiome in the lower respiratory tract of horses with equine asthma

Libor Podojil1, Vendula Jandová2, Zuzana Drábková1, Dára Brabcová1, Markéta Sedlinská1, Štěpán Bodeček1, Eva Jánová3,4, Soňa Peková5

1University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Equine Clinic, Czech Republic
2Equine Internal Medicine Services, Nákle, Czech Republic
3University of Veterinary Sciences Brno, CEITEC, Czech Republic
4University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Department of Animal Genetics, Czech Republic
5Tilia Laboratories, Pchery, Czech Republic

Received April 1, 2023
Accepted October 25, 2023

References

1. Beeler-Marfisi J, Clark ME, Wen X, Sears W, Huber L, Ackerley C, Viel L, Bienzle D 2010: Experimental induction of recurrent airway obstruction with inhaled fungal spores, lipopolysacharide, and silica microspheres in horses. Am J Vet Res 71: 682-689 <https://doi.org/10.2460/ajvr.71.6.682>
2. Bond SL, Timsit E, Workentine M, Alexander T, Léguillette R 2017: Upper and lower respiratory tract microbiota in horses: bacterial communities associated with health and mild asthma (inflammatory airway disease) and effects of dexamethasone. BMC Microbiol 17: 1-11 <https://doi.org/10.1186/s12866-017-1092-5>
3. Bond SL, Workentine M, Hundt J, Gilkerson JR, Léguillette R 2020: Effects of nebulized dexamethasone on the respiratory microbiota and mycobiota and relative equine herpesvirus‐1, 2, 4, 5 in an equine model of asthma. J Vet Intern Med 34: 307-321 <https://doi.org/10.1111/jvim.15671>
4. Couëtil LL, Cardwell JM, Gerber V, Lavoie JP, Léguillette R, Richard EA 2016: Inflammatory airway disease of horses—revised consensus statement. J Vet Intern Med 30: 503-515 <https://doi.org/10.1111/jvim.13824>
5. Couetil LL, Cardwell JM, Leguillette R, Mazan M, Richard E, Bienzle D, Bullone M, Gerber V, Ivester K, Lavoie J-P, Martin J, Moran G, Niedźwiedź A, Pusterla N, Swiderski C 2020: Equine asthma: current understanding and future directions. Front Vet Sci 7: 450 <https://doi.org/10.3389/fvets.2020.00450>
6. Dauvillier J, Ter Woort F, van Erck‐Westergren E 2019: Fungi in respiratory samples of horses with inflammatory airway disease. J Vet Intern Med 33: 968-975 <https://doi.org/10.1111/jvim.15397>
7. Day BJ, Hatch A, Carstens DD, Crapo JD 1995: An easy and economical method to prepare cells for cytologic analyses. J Pharmacol Toxicol Methods 34: 57-62 <https://doi.org/10.1016/1056-8719(95)00029-H>
8. Dobiáš R, Jahn P, Tóthová K, Dobešová O, Višňovská D, Patil R, Škríba A, Jaworská P, Škorič M, Podojil L, Kantorová M, Mrázek J, Krejčí E, Stevens DA, Havlíček V 2023: Diagnosis of aspergillosis in horses. J Fungi 9: 161 <https://doi.org/10.3390/jof9020161>
9. Gerber V, Lindberg Å, Berney C, Robinson NE 2004: Airway mucus in recurrent airway obstruction–short‐term response to environmental challenge. J Vet Intern Med. 18: 92-97
10. Hotchkiss JW, Reid SWJ, Christley RM 2007: A survey of horse owners in Great Britain regarding horses in their care. Part 2: Risk factors for recurrent airway obstruction. Equine Vet J 39: 301-308 <https://doi.org/10.2746/042516407X180129>
11. Huang YJ, Nelson CE, Brodie EL, Desantis TZ, Baek MS, Liu J, Woyke T, Allgaier M, Bristow J, Wiener-Kronish JP, Sutherland ER, King TS, Icitovic N, Martin RJ, Calhoun WJ, Castro M, Denlinger LC, Dimango E, Kraft M, Peters SP, Wasserman SI, Wechsler ME, Boushey HA, Lynch SV, National Heart, Lung, and Blood Institute's Asthma Clinical Research Network 2011: Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma. J Allergy Clin Immunol 127: 372-381 <https://doi.org/10.1016/j.jaci.2010.10.048>
12. Ivester KM, Couëtil LL, Moore GE 2018: An observational study of environmental exposures, airway cytology, and performance in racing thoroughbreds. J Vet Intern Med 32: 1754-1762 <https://doi.org/10.1111/jvim.15226>
13. Lemonnier LC, Couroucé A, Cessans M, Petit L, Cardwell JM, Toquet MP, Richard EA 2022: Variability of fungi detection in the respiratory tract of racehorses according to the sampling site. 11th International Conference on Equine Exercise Physiology, Vol. 18, No. Supp 1
14. Manguin E, Pépin E, Boivin R, Leclere M 2020: Tracheal microbial populations in horses with moderate asthma. J Vet Intern Med 34: 986-995 <https://doi.org/10.1111/jvim.15707>
15. Načeradská M, Peková S, Danesi P, Furlanello T, Calleo R, Martin P, Ike F, Malik R 2021: A novel Filobacterium sp can cause chronic bronchitis in cats. PLoS One 16: e0251968 <https://doi.org/10.1371/journal.pone.0251968>
16. Rick EM, Woolnough K, Pashley CH, Wardlaw AJ 2016: Allergic fungal airway disease. J Investig Allergol Clin Immunol 26: 344-354 <https://doi.org/10.18176/jiaci.0122>
17. Schildgen V, Mai S, Khalfaoui S, Lüsebrink J, Pieper M, Tillmann RL, Brockmann M Schildgen O 2014: Pneumocystis jirovecii can be productively cultured in differentiated CuFi-8 airway cells. mBio 5: e01186-14 <https://doi.org/10.1128/mBio.01186-14>
18. Wasko AJ, Barkema HW, Nicol J, Fernandez N, Logie N, Léguillette R 2011: Evaluation of a risk‐screening questionnaire to detect equine lung inflammation: results of a large field study. Equine Vet J 43: 145-152 <https://doi.org/10.1111/j.2042-3306.2010.00150.x>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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