Acta Vet. Brno 2024, 93: 3-10

Efficacy of targeted therapy of environmental mastitis using on-farm culturing in small dairy herds

Josef Prášek1, Bohdana Řezníčková1, Gabriela Malá2, Pavel Novák2, Jiří Smola1

1Veterinary University Brno, Faculty of Veterinary Medicine, Ruminant and Swine Clinic, Brno, Czech Republic
2Institute of Animal Sciences, Livestock Technology and Management, Prague Uhříněves, Czech Republic

Received November 6, 2023
Accepted February 19, 2024

The aim of the study was to evaluate treatment protocols for improvement of clinical and bacteriological cure rate of non-severe clinical mastitis (CM) and selective dry cow therapy (SDCT). Mastitis management based on on-farm commercial culture system MicroMastTM and data analysis were implemented into two dairy herds. Quarters with evidence of Gram-positive agents were treated using benzyl penicillin or amoxicillin and/or by amoxicillin with clavulanic acid. In herd A, 31 quarters of non-severe CM were recorded. Of them, Streptococcus uberis was isolated in 16 (51.6%), non-aureus staphylococci (NAS) in 8 (25.8%), E. coli in 5 (16.1%) and no growth in 2 (6.5%) cases. Bacteriological cure was observed in 24/29 (82.8%) quarters. Antimicrobial consumption was reduced from 3.56 to 2.33 doses per case. Under the SDCT protocol, 28 quarters (13.2%) of 53 cows dried-off were included, achieving a reduction by 86.8% on the quarter level. In herd B, 23 quarters of non-severe CM were recorded. Of them, S. uberis and Escherichia coli were identified equally in 8 (34.8%) samples and NAS in 2 (8.7%) samples and with no growth in 5 (21.7%) cases. Bacteriological cure was achieved in 17/18 quarters (94.4%). Antimicrobial consumption was reduced from 4.45 to 1.83 doses per case. Only 5 (1.7%) quarters of 72 cows were included for SDCT with reduction in consumption of antimicrobials by 98.3% on the quarter level. In summary, innovated treatment protocols based on results of on-farm culture enabled a significant reduction of antimicrobial consumption and improvement of bacteriological cure rate in conditions of practise.


The study was supported by the project NAZV MZe ČR QK 21020304: The influence of the level of management of livestock breeding and disease prevention, including biosecurity, on the reduction of antimicrobial consumption and the spread of antimicrobial resistance.


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