Acta Vet. Brno 2024, 93: 281-287

https://doi.org/10.2754/avb202493030281

Effect of different types of exercise on salivary biochemical indices in the horse

Lucie Hostovská1, Martin Hostovský2, Katarina Nenadović3, Eva Voslářová1, Vladimír Večerek1

1University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Protection and Welfare and Veterinary Public Health, Brno, Czech Republic
2University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Department of Physiology, Brno, Czech Republic
3University of Belgrade, Faculty of Veterinary Medicine, Department of Animal Hygiene, Belgrade, Serbia

Received June 5, 2024
Accepted July 11, 2024

References

1. Ayala I, Martos NF, Silvan G, Gutierrez-Panizo C, Clavel JG, Illera JC 2012: Cortisol, adrenocorticotropic hormone, serotonin, adrenaline and noradrenaline serum concentrations in relation to disease and stress in the horse. Res Vet Sci 93: 103-107 <https://doi.org/10.1016/j.rvsc.2011.05.013>
2. Bottegaro NB, Gotic J, Suran J, Brozic D, Klobucar K, Bojanic K, Vrbanac Z 2018: Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Vet Res 14: 216 <https://doi.org/10.1186/s12917-018-1540-y>
3. Campbell MLH 2013: When does use become abuse in equestrian sport? Equine Vet Educ 25: 489-492 <https://doi.org/10.1111/eve.12087>
4. Contreras-Aguilar MD, Escribano D, Quiles A, Lopez-Arjona M, Ceron JJ, Martinez-Subiela S, Hevia ML, Tecles F 2019a: Evaluation of new biomarkers of stress in saliva of sheep. Animal 13: 1278-1286 <https://doi.org/10.1017/S1751731118002707>
5. Contreras-Aguilar MD, Escribano D, Martinez-Subiela S, Martin-Cuervo M, Lamy E, Tecles F, Ceron JJ 2019b: Changes in saliva analytes in equine acute abdominal disease: a sialochemistry approach. BMC Vet Res 15: 9 <https://doi.org/10.1186/s12917-019-1933-6>
6. Contreras-Aguilar MD, Cerón JJ, Muńoz A, Ayala I 2021: Changes in saliva biomarkers during a standardized increasing intensity field exercise test in endurance horses. Animal 15
7. Cook CJ, Jacobson LH 1995: Salivary cortisol as an indicator of stress in sheep (Ovis ovis). New Zeal Vet J 43: 248 <https://doi.org/10.1080/00480169.1995.35902>
8. Cook NJ, Hayne SM, Rioja-Lang FC, Schaefer AL, Gonyou HW 2013: The collection of multiple saliva samples from pigs and the effect on adrenocortical activity. Can J Anim Sci 93: 329-333 <https://doi.org/10.4141/cjas2012-120>
9. Dashper K 2017: Listening to horses: Developing attentive interspecies relationships through sport and leisure. Soc Anim 25: 207-224 <https://doi.org/10.1163/15685306-12341426>
10. Douglas J, Owers R, Campbell MLH 2022: Social licence to operate: What can equestrian sports learn from other industries? Animals 12: 1987 <https://doi.org/10.3390/ani12151987>
11. Fazio F, Casella S, Assenza A, Arfuso F, Tosto F, Piccione G 2014: Blood biochemical changes in show jumpers during a simulated show jumping test. Vet Arhiv 84: 143-152
12. Gomes CLN, Alves AM, Ribeiro JD, Moraes FJ, Barreto RA, Fucuta RS, Ribeiro BM, Miranda LM 2020: Physiological and biochemical responses and hydration status in equines after two barrel racing courses. Pesqui Vet Brasil 40: 992-1001 <https://doi.org/10.1590/1678-5150-pvb-6710>
13. Hura V, Novotny F, Boldizár M, Rédl M, Noskovicová J, Hornák S, Petrovic V, Lazar G, Kovác G 2013: Biochemical responses to a non-standard exercise in horses trained for jumping. Acta Vet Brno 82: 161-167 <https://doi.org/10.2754/avb201382020161>
14. Kim JS, Hinchcliff KW, Yamaguchi M, Beard LA, Markert CD, Devor ST 2005: Exercise training increases oxidative capacity and attenuates exercise-induced ultrastructural damage in skeletal muscle of aged horses. J Appl Physiol 98: 334-342 <https://doi.org/10.1152/japplphysiol.00172.2003>
15. Lefebvre HP, Laroute V, Braun JP, Lassourd V, Toutain PL 1996: Non-invasive and quantitative evaluation of post-injection muscle damage by pharmacokinetic analysis of creatine kinase release. Vet Res 27: 343-361
16. Marlin D, Nankervis KJ 2013: Equine exercise physiology. Wiley-Blackwell, Oxford, 304 p.
17. Masko M, Domino M, Jasinski T, Witkowska-Pilaszewicz O 2021: The physical activity-dependent hematological and biochemical changes in school horses in comparison to blood profiles in endurance and race horses. Animals 11: 13
18. Massányi M, Halo M, Massányi P, Mlyneková E, Greń A, Formicki G, Halo M 2022: Changes in haematological and biochemical parameters in blood serum of horses during exposition to workload stress. Heliyon 8: e12241 <https://doi.org/10.1016/j.heliyon.2022.e12241>
19. McGowan CM, Whitworth DJ 2008: Overtraining syndrome in horses. Comp Exerc Physiol 5: 57-65 <https://doi.org/10.1017/S1478061508979202>
20. Mukai K, Takahashi T, Eto D, Ohmura H, Tsubone H, Hiraga A 2007: Heart rates and blood lactate response in Thorougbred horses during a race. J Equine Sci 18: 58 <https://doi.org/10.1294/jes.18.153>
21. Munoz A, Riber C, Santisteban R, Rubio MD, Aguera EI, Castejon FM 1999: Cardiovascular and metabolic adaptations in horses competing in cross-country events. J Vet Med Sci 61: 13-20 <https://doi.org/10.1292/jvms.61.13>
22. Nemecková M, Popelková T, Chloupek P 2022: Sampling methods for determination of cortisol in pig saliva and their use in the assessment of pig welfare. Acta Vet Brno 91: 261-266 <https://doi.org/10.2754/avb202291030261>
23. Ohkawa H, Ohishi N, Yagi K 1979: Assay for lipid peroxides in animal-tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358 <https://doi.org/10.1016/0003-2697(79)90738-3>
24. Popelkova T, Nemeckova M, Tsponova Z, Pistekova V 2022: Factors influencing the welfare of dairy goats. Acta Vet Brno 91: 333-337 <https://doi.org/10.2754/avb202291040333>
25. Pösö AR, Hyyppä S 1999: Metabolic and hormonal changes after exercise in relation to muscle glycogen concentrations. Equine Vet J 31: 332-336 <https://doi.org/10.1111/j.2042-3306.1999.tb05244.x>
26. Rubio CP, Mainau E, Ceron JJ, Contreras-Aguilar MD, Martinez-Subiela S, Navarro E, Tecles F, Manteca X, Escribano D 2019: Biomarkers of oxidative stress in saliva in pigs: analytical validation and changes in lactation. BMC Vet Res 15: 7 <https://doi.org/10.1186/s12917-019-1875-z>
27. Santos SA, Silva R, Azevedo JRM, Mello MAR, Soares AC, Sibuya SY, Anaruma CA 2001: Serum electrolyte and total protein alterations in Pantaneiro horse during long distance exercise. Arq Bras Med Vet Zoo 53: 351-357 <https://doi.org/10.1590/S0102-09352001000300013>
28. Sheriff MJ, Dantzer B, Delehanty B, Palme R, Boonstra R 2011: Measuring stress in wildlife: techniques for quantifying glucocorticoids. Oecologia 166: 869-887 <https://doi.org/10.1007/s00442-011-1943-y>
29. Siqueira RF, Weigel RA, Nunes GR, Mori CS, Fernandes WR 2014: Oxidative profiles of endurance horses racing different distances. Arq Bras Med Vet Zoo 66: 455-461 <https://doi.org/10.1590/1678-41625760>
30. Smarsh DN, Williams CA 2017: Oxidative stress and antioxidant status in standardbreds: effect of age and training in resting plasma and muscle. J Equine Vet Sci 48: 93-102 <https://doi.org/10.1016/j.jevs.2016.07.020>
31. St George L, Hobbs SJ, Sinclair J, Richards J, Roddam H 2019: Does equestrian knowledge and experience influence selection and training practices for showjumping horses? Comp Exerc Physiol 15: 123-135 <https://doi.org/10.3920/CEP180049>
32. Stepanova LV, Kolesnik OV, Kolenchukova OA, Fedotova AS, Kolomeytsev AV, Makarov AV, Kratasyuk VA 2023: The prospects for using bioluminescent enzymatic analysis to reveal the exercise capacity of sports horses. Biology Bull 50: 52-60 <https://doi.org/10.1134/S1062359023010119>
33. Strzelec K, Kankofer M, Pietrzak S 2011: Cortisol concentration in the saliva of horses subjected to different kinds of exercise. Acta Vet Brno 80: 101-105 <https://doi.org/10.2754/avb201180010101>
34. Svestkova M, Pistekova V, Takacova D, Vecerek V, Voslarova V 2024: Analysis of the major deficiencis detected during welfare inspections of farm animals in the Czech Republic. Acta Vet Brno 93: 45-57 <https://doi.org/10.2754/avb202493010045>
35. Tabak LA 2004: Dental, oral, and craniofacial research: the view from the NIDCR. J Dent Res 83: 196-197 <https://doi.org/10.1177/154405910408300301>
36. Tateo A, Padalino B, Boccaccio M, Maggiolino A, Centoducati P 2012: Transport stress in horses: Effects of two different distances. J Vet Behav 7: 33-42 <https://doi.org/10.1016/j.jveb.2011.04.007>
37. Teixeira-Neto AR, Ferraz GC, Moscardini ARC, Balsamao GM, Souza JCF, Queiroz-Neto A 2008: Alterations in muscular enzymes of horses competing long-distance endurance rides under tropical climate. Arq Bras Med Vet Zoo 60: 543-549 <https://doi.org/10.1590/S0102-09352008000300004>
38. Thoma MV, Kirschbaum C, Wolf JM, Rohleder N 2012: Acute stress responses in salivary alpha-amylase predict increases of plasma norepinephrine. Biol Psychol 91: 342-348 <https://doi.org/10.1016/j.biopsycho.2012.07.008>
39. Wing C 2019: A brief review of salivary biomarkers as stress indicators in sport and exercise. Strength Cond J 41: 80-88 <https://doi.org/10.1519/SSC.0000000000000435>
40. Witkowska-Pilaszewicz O, Grzedzicka J, Sen J, Czopowicz M, Zmigrodzka M, Winnicka A, Cywinska A, Carter C 2021: Stress response after race and endurance training sessions and competitions in Arabian horses. Prev Vet Med 188: 5 <https://doi.org/10.1016/j.prevetmed.2021.105265>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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