Acta Vet. Brno 2024, 93: 321-330

https://doi.org/10.2754/avb202493030321

The effect of aldehyde fixatives, alcohol-based fixatives, and fixatives combining denaturation and crosslinking on histomorphological, histochemical, and molecular genetic studies during short-term and long-term fixation: a comprehensive analysis

Dominika Macháčová1, Petra Konečná1, František Tichý1, Michal Kyllar2, Monika Šedivcová3, Luděk Fiala4,5,6, Jiří Lenz1,6,7

1University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Department of Anatomy, Histology and Embryology, Brno, Czech Republic
2University of Veterinary Medicine Vienna, Institute of Morphology, Department of Pathobiology, Vienna, Austria
3Bioptical Laboratory Ltd., Pilsen, Czech Republic
4Charles University Pilsen, Department of Sexology, Psychiatric Clinic, Faculty of Medicine, Pilsen, Czech Republic
5Charles University Prague, Institute of Sexology, First Faculty of Medicine, Prague, Czech Republic
6Cytohisto s.r.o., Břeclav, Czech Republic
7Znojmo Hospital, Department of Pathology, Znojmo, Czech Republic

Received May 3, 2024
Accepted July 11, 2024

References

1. Bancroft JD, Stevens A 1996: Theory and Practice of Histological Techniques. Churchill Livingstone, Edinburg, 766 p.
2. Bindhu PR, Krishnapillai R, Thomas P, Jayanthi P 2013: Facts in artifacts. Oral Maxillofac Pathol 17: 397-401 <https://doi.org/10.4103/0973-029X.125206>
3. Bonin S, Petrera F, Rosai J, Stanta G 2005: DNA and RNA obtained from Bouin’s fixed tissues. J Clin Pathol 58: 313-316 <https://doi.org/10.1136/jcp.2004.016477>
4. Chung JY, Song JS, Ylaya K, Sears JD, Choi L, Cho H, Rosenberg AZ, Hewitt SM 2018: Histomorphological and molecular assessments of the fixation times comparing formalin and ethanol-based fixatives. J Histochem Cytochem 66: 121-135 <https://doi.org/10.1369/0022155417741467>
5. Eltoum I, Fredenburgh J, Myers RB 2001: Introduction to the theory and practice of fixation of tissues. J Histotechnol 24: 173-190 <https://doi.org/10.1179/his.2001.24.3.173>
6. Fox CH, Johnson FB, Whiting J, Roller PP 1985: Formaldehyde fixation. J Histochem Cytochem 33: 845-853 <https://doi.org/10.1177/33.8.3894502>
7. Gatta LB, Cadei M, Balzarini P, Castriciano S, Paroni R, Verzeletti A, Cortellini V, De Ferrari F, Grigolato P 2012: Application of alternative fixatives to formalin in diagnostic pathology. Eur J Histochem 56: e12 <https://doi.org/10.4081/ejh.2012.12>
8. Howat WJ, Wilson BA 2014: Tissue fixation and the effect of molecular fixatives on downstream staining procedures. Methods 70: 12-19 <https://doi.org/10.1016/j.ymeth.2014.01.022>
9. Kap M, Smedts F, Oosterhuis W, Winther R, Christensen N, Reischauer B, Viertler C, Groelz D, Becker KF, Zatloukal K, Langer R, Slotta-Huspenina J, Bodo K, de Jong B, Oelmuller U, Riegman P 2011: Histological assessment of PAXgene tissue fixation and stabilization reagents. PLoS One 6: e27704 <https://doi.org/10.1371/journal.pone.0027704>
10. Lenz J, Machacova D, Konecna P, Fiala L, Kyllar M, Tichy F 2022a: Effects of different fixatives over different fixation times, including Antigenfix, on immunohistochemical studies. Acta Vet Brno 91: 179-188 <https://doi.org/10.2754/avb202291020179>
11. Lenz J, Konecna P, Tichy F, Machacova D, Fiala L, Hurnik P, Kyllar M 2022b: Unique expression patterns of the embryonal stem cell marker SOX2 and hormone receptors suggest the existence of a subpopulation of epithelial stem/progenitor cells in porcine and bovine endometrium. Vet Med Sci 8: 1489-1501 <https://doi.org/10.1002/vms3.802>
12. Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z 2011: Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cellimplanted mouse models. J Histochem Cytochem 59: 68-75 <https://doi.org/10.1369/jhc.2010.957217>
13. Meecham A, Miranda E, Morris HT, Hair J, Oien KA, Gerrard G, Guppy N, Mooney D, Shaw EC, Ashton-Key M, Lees R, Flanagan A, Rodriguez-Justo M 2021: Alternative tissue fixation for combined histopathological and molecular analysis in a clinically representative setting. J Histochem Cell Biol 156: 595-607
14. Netto GJ, Saad RD, Dysert PA 2nd 2003: Diagnostic molecular pathology: current techniques and clinical applications, part I. Proc (Bayl Univ Med Cent) 16: 379-83
15. Oboma YI, Ngokere AA 2018: Effect of formalin fixation on DNA purity and quantity, nucleic acid, and amplicon size in cervical human papilloma virus detection. Afr J Cell Path 10: 47-53 <https://doi.org/10.5897/AJCPath2018.0009>
16. Srinivasan M, Sedmak D, Jewell S 2002: Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 161: 1961-1971 <https://doi.org/10.1016/S0002-9440(10)64472-0>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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