Acta Vet. Brno 2025, 94: 77-86

https://doi.org/10.2754/avb202594010077

A direct method for determination of the pure muscle protein content in dry fermented sausages and cooked ham using creatinine adsorption

František Ježek, Jiří Bednář, Jana Doležalová, Josef Kameník, Klára Bartáková, Michaela Králová

University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Origin Food & Gastronomic Sciences

Received December 9, 2024
Accepted January 6, 2025

References

1. Adamczyk B, Simon J, Kitunen V, Adamczyk S, Smolander A 2017: Tannins and their complex interaction with different organic nitrogen compounds and enzymes: Old paradigms versus recent advances. ChemistryOpen 6: 610-614 <https://doi.org/10.1002/open.201700113>
2. Aro Aro JM, Nyam-Osor P, Tsuji K, Shimada K, Fukushima M, Sekikawa M 2010: The effect of starter cultures on proteolytic changes and amino acid content in fermented sausages. Food Chem 119: 279-285 <https://doi.org/10.1016/j.foodchem.2009.06.025>
3. Berardo A, Devreese B, De Maere H, Stavropoulou DA, Van Royen G, Leroy F, De Smet S 2017 Actin proteolysis during ripening of dry fermented sausages at different pH values. Food Chem 221: 1322-1332 <https://doi.org/10.1016/j.foodchem.2016.11.023>
4. Boutten B, Brazier M, Morche N, Morel A, Vendeuvre JL 2000: Effects of animal and muscle characteristics on collagen and consequences for ham production. Meat Sci 55: 233-238 <https://doi.org/10.1016/S0309-1740(99)00148-5>
5. Dahl O 1963: Meat quality measurement, creatine content as an index of quality of meat products. J Agric Food Chem 11: 350-355 <https://doi.org/10.1021/jf60128a026>
6. Decree No. 69/2016 Coll. of Czech Republic of 17 February 2016 on requirements for meat, meat products, fishery and aquaculture products and products thereof, eggs and products thereof.
7. Del Campo G, Gallego B, Berregi I, Casado A 1998: Creatinine, creatine and protein in cooked meat products. Food Chem 63: 187-190 <https://doi.org/10.1016/S0308-8146(98)00034-X>
8. Di Cagno, R, López CC, Tofalo R, Gallo G, De Angelis M, Paparella A, Hammes WP, Gobetti M 2008: Comparison of the compositional, microbiological, biochemical and volatile profile characteristics of three Italian PDO fermented sausages. Meat Sci 79: 224-235 <https://doi.org/10.1016/j.meatsci.2007.09.006>
9. Díaz O, Fernandez M, Garcia de Fernando GD, de la Hoz L, Ordoñez JA 1997: Proteolysis in dry fermented sausages: The effect of selected exogenous proteases. Meat Sci 46: 115-128 <https://doi.org/10.1016/S0309-1740(97)00013-2>
10. Dvořák Z 1981: Creatine as an indicator of net muscle proteins. J Sci Food Agric 32: 1033-1036 <https://doi.org/10.1002/jsfa.2740321013>
11. Feiner, G 2006: Meat products handbook. Practical science and technology. Woodhead publishing limited, Cambridge, 648 p.
12. Hajšlová J, Jírů M, Kocourek V, Stránská M 2018: Methodology for determining the content of pure muscle protein in meat products. Certified methodology (In Czech). University of chemistry and technology Prague, 34 p.
13. Holck A, Heir E, Johannessen TC, Axelsson L (2015) Northern European products. In: Toldrá F (Ed.): Handbook of fermented meat and poultry, 2nd edn. John Wiley & Sons, Oxford, pp 313-320
14. Hughes MC, Kerry JP, Arendt EK, Kenneally PM, McSweeney PLH, O’Neill EE 2002: Characterization of proteolysis during the ripening of semi-dry fermented sausages. Meat Sci 62: 205-216 <https://doi.org/10.1016/S0309-1740(01)00248-0>
15. Hulánková R, Kameník J, Saláková A, Závodský D, Borilova G 2018: The effect of dry aging on instrumental, chemical and microbiological parameters of organic beef loin muscle. LWT 89: 559-565 <https://doi.org/10.1016/j.lwt.2017.11.014>
16. Ikonić P, Tasić T, Petrović L, Škaljac S, Jokanović M, Mandić A, Ikonić B 2013: Proteolysis and biogenic amines formation during the ripening of Petrovská klobása, traditional dry-fermented sausage from Northern Serbia. Food Control 30: 69-75 <https://doi.org/10.1016/j.foodcont.2012.06.021>
17. ISO 1442:1997 Meat and meat products – Determination of moisture content (Reference method). International Organization for Standartization, Genève, 4 p.
18. Kameník J, Macharáčková B, Ježek F, Bednář J, Doležalová J 2023: A mini review of proteolysis in dry fermented sausages. Fleischwirtschaft 103: 62-67
19. Kashani K, Rosner MH, Ostermann M 2020: Creatinine: From physiology to clinical application. Eur J Intern Med 72: 9-14 <https://doi.org/10.1016/j.ejim.2019.10.025>
20. Kauffman RG 2012: Meat Composition. In: Hui YH (Ed.): Handbook of Meat and Meat Processing, 2nd edn. CRC Press, Boca Raton, pp 45-61
21. Kong Y, Feng M, Sun J 2020: Effects of Lactobacillus plantarum CD101 a Staphylococcus simulans NJ201 on proteolytic changes and bioactivities (antioxidant and antihypertensive activities) in fermented pork sausage. LWT – Food Sci Technol 133: 109985 <https://doi.org/10.1016/j.lwt.2020.109985>
22. Kotaška K, Jedličková B, Průša R 2008: Is the assessment of serum creatinine reliable? Cas Lek Cesk 147: 392-395
23. Kvasnička F 2003: Compendium of methodologies developed as part of the solution to project No. EP9179 Methods for assessing compliance and detecting food adulteration (In Czech). University of Chemistry and Technology Prague, 18 p.
24. Kvasnička F, Voldřich M 2000: Isotachophoretic determination of creatinine in meat and meat products. Electrophoresis 21: 2848-2850 <https://doi.org/10.1002/1522-2683(20000801)21:14<2848::AID-ELPS2848>3.0.CO;2-N>
25. Lee S, Choi L-S, Jo K, Yong HI, Jeong HG, Jung S 2021: Improvement of meat protein digestibility in infants and the elderly. Food Chem 356: 129707 <https://doi.org/10.1016/j.foodchem.2021.129707>
26. Ljungh Á, Wadström T 2009: Lactobacillus molecular biology: From genomics to probiotics. Caister Academic Press, 205 p.
27. Los FGB, Granato D, Prestes RC, Demiate IM 2014: Characterization of commercial cooked hams according to physicochemical, sensory, and textural parameters using chemometrics. Food Sci Technol 34: 577-584 <https://doi.org/10.1590/1678-457x.6423>
28. Meza-Márquez OG, Gallardo-Velázquez T, Osorio-Revilla G 2010: Application of mid-infrared spectroscopy with multivariate analysis and soft independent modelling of class analogies (SIMCA) for the detection of adulterants in minced beef. Meat Sci 86: 511-519 <https://doi.org/10.1016/j.meatsci.2010.05.044>
29. Mohabbati-Kalejahi E, Azimirad V, Bahrami M, Ganbari A 2012: A review on creatinine measurement techniques. Talanta 97: 1-8 <https://doi.org/10.1016/j.talanta.2012.04.005>
30. Mora L, Hernández-Cázares AS, Sentandreu MA, Toldrá F 2010: Creatine and creatinine evolution during the processing of dry-cured ham. Meat Sci 84: 384-389 <https://doi.org/10.1016/j.meatsci.2009.09.006>
31. Mora L, Sentandreu MÁ, Toldrá F 2008a: Contents of creatine, creatinine and carnosine in porcine muscles of different metabolic types. Meat Sci 79: 709-715 <https://doi.org/10.1016/j.meatsci.2007.11.002>
32. Mora L, Sentandreu MÁ, Toldrá F 2008b: Effect of cooking conditions on creatinine formation in cooked ham. J Agric Food Chem 56: 11279-11284 <https://doi.org/10.1021/jf801953t>
33. Snider S, Baldwin RH 1981: Flavor intensity as related to the creatine and creatinine content of microwave and conventionally cooked beef. J Food Sci 46: 1801-1804 <https://doi.org/10.1111/j.1365-2621.1981.tb04489.x>
34. Toldrá F, Reig M 2015: The biochemistry of meat and fat. In: Toldrá F (Ed.): Handbook of fermented meat and poultry, 2nd edn, John Wiley & Sons, Oxford, pp 49-54
35. Utrera M, Armenteros M, Ventanas S, Solano F, Estévez M 2012: Pre-freezing raw hams affects quality traits in cooked hams: Potential influence of protein oxidation. Meat Sci 92: 596-603 <https://doi.org/10.1016/j.meatsci.2012.06.005>
36. Válková V, Saláková A, Buchtová H, Tremlová B 2007: Chemical, instrumental and sensory characteristics of cooked pork ham. Meat Sci 77: 608-615 <https://doi.org/10.1016/j.meatsci.2007.05.013>
37. Wyss M, Kaddurah-Daouk R 2000: Creatine and creatinine metabolism. Physiol Rev 80: 1107-1213 <https://doi.org/10.1152/physrev.2000.80.3.1107>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Archive