Acta Vet. Brno 2025, 94: 111-118

https://doi.org/10.2754/avb202594020111

The effect of different concentrations of microalga Chlorella vulgaris supplementation on ruminal fermentation and blood indices in cows

Svetlana Malyugina1,2

1Agrovýzkum Rapotín Ltd., Vikýřovice, Czech Republic
2Mendel University in Brno, Faculty of AgriSciences, Department of Animal Nutrition and Forage Production, Brno, Czech Republic

Received May 21, 2025
Accepted July 8, 2025

References

1. Altomonte I, Salari F, Licitra R, Martini M 2018: Use of microalgae in ruminant nutrition and implications on milk quality–A review. Livest Sci 214: 25-35 <https://doi.org/10.1016/j.livsci.2018.05.006>
2. Bach A, Calsamiglia S, Stern MD 2005: Nitrogen metabolism in the rumen. J Dairy Sci 88: E9-E21 <https://doi.org/10.3168/jds.S0022-0302(05)73133-7>
3. Baraka T 2012: Comparative studies of rumen pH, total protozoa count, generic and species composition of ciliates in camel, buffalo, cattle, sheep and goat in Egypt. J Amer Sci 8: 655-669
4. Bature A, Melville L, Rahman KM, Aulak P 2022: Microalgae as feed ingredients and a potential source of competitive advantage in livestock production: A review. Livest Sci 259: 104907 <https://doi.org/10.1016/j.livsci.2022.104907>
5. Becker EW 2007: Micro-algae as a source of protein. Biotech adv 25: 207-210 <https://doi.org/10.1016/j.biotechadv.2006.11.002>
6. Bhatta R, Tajima K, Kurihara M 2006: Influence of temperature and pH on fermentation pattern and methane production in the rumen simulating fermenter (RUSITEC). Asian-Australas J Anim Sci 19: 376-380 <https://doi.org/10.5713/ajas.2006.376>
7. Boeckaert C, Mestdagh J, Vlaeminck B, Clayton D, Fievez V 2006: Microalgae as potent rumen methane inhibitors and modifiers of rumen lipolysis and biohydrogenation of linoleic and linolenic acid. Proc. International Congress Series 1293: 184-188 <https://doi.org/10.1016/j.ics.2006.01.016>
8. Boeckaert C, Vlaeminck B, Dijkstra J, Issa-Zacharia A, Van Nespen T 2008: Effect of dietary starch or micro algae supplementation on rumen fermentation and milk fatty acid composition of dairy cows. J Dairy Sci 91: 4714-4727 <https://doi.org/10.3168/jds.2008-1178>
9. Calsamiglia S, Ferret A, Devant M 2002: Effects of pH and pH fluctuations on microbial fermentation and nutrient flow from a dual-flow continuous culture system. J Dairy Sci 85: 574-579 <https://doi.org/10.3168/jds.S0022-0302(02)74111-8>
10. Chen G, Russell J, Sniffen C 1987: A procedure for measuring peptides in rumen fluid and evidence that peptide uptake can be a rate-limiting step in ruminal protein degradation. J Dairy Sci 70: 1211-1219 <https://doi.org/10.3168/jds.S0022-0302(87)80133-9>
11. Choi YY, Shin NH, Lee SJ, Lee YJ, Kim HS 2021: In vitro five brown algae extracts for efficiency of ruminal fermentation and methane yield. J Appl Phycol 33: 1253-1262 <https://doi.org/10.1007/s10811-020-02361-4>
12. Commission Regulation 2009: Commission Regulation (EC) No. 152/2009 laying down the methods of sampling and analysis for the official control of feed. Off J Eur Union L 54: 1-8
13. Dehority BA 2004: Rumen Microbiology: Burk A Dehority (Ed.), Nottingham University Press, Nottingham, 372 p.
14. Dehority BA 2018: Laboratory manual for classification and morphology of rumen ciliate protozoa. CRC Press, USA, 127 p.
15. Elghandour M, Vallejo L, Salem A, Salem M, Camacho L, Odongo N 2017: Effects of Schizochytrium microalgae and sunflower oil as sources of unsaturated fatty acids for the sustainable mitigation of ruminal biogases methane and carbon dioxide. J Clean Prod 168: 1389-1397 <https://doi.org/10.1016/j.jclepro.2017.09.039>
16. Eugène M, Archimède H, Sauvant D 2004: Quantitative meta-analysis on the effects of defaunation of the rumen on growth, intake and digestion in ruminants. Livest Prod Sci 85: 81-97 <https://doi.org/10.1016/S0301-6226(03)00117-9>
17. Filípek J, Dvořák R 2009: Determination of the volatile fatty acid content in the rumen liquid: comparison of gas chromatography and capillary isotachophoresis. Acta Vet Brno 78: 627-633 <https://doi.org/10.2754/avb200978040627>
18. Ghwenm SS, Kata FS, Athbi AM 2020: Hypoglycemic and antioxidant effect of the ethanol extract of Chlorella vulgaris inalloxan-induced diabetes mice. Bioch Cell Arch 20: 3535-3542
19. Glover K, Budge S, Rose M, Rupasinghe H, MacLaren L 2012: Effect of feeding fresh forage and marine algae on the fatty acid composition and oxidation of milk and butter. J Dairy Sci 95: 2797-2809 <https://doi.org/10.3168/jds.2011-4736>
20. Henderson G, Cox F, Ganesh S, Jonker A, Young W, Janssen PH 2015: Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep 5: 14567 <https://doi.org/10.1038/srep14567>
21. Kholif AE, Olafadehan OA 2021: Chlorella vulgaris microalgae in ruminant nutrition: a review of the chemical composition and nutritive value. Ann Anim Sci 21: 789-806 <https://doi.org/10.2478/aoas-2020-0117>
22. Kopecny J, Wallace RJ 1982: Cellular location and some properties of proteolytic enzymes of rumen bacteria. Appl Environ Microbiol 43: 1026-1033 <https://doi.org/10.1128/aem.43.5.1026-1033.1982>
23. Kusmayadi A, Leong YK, Yen H-W, Huang C-Y, Chang J-S 2021: Microalgae as sustainable food and feed sources for animals and humans–biotechnological and environmental aspects. Chemosphere 271: 129800 <https://doi.org/10.1016/j.chemosphere.2021.129800>
24. Luther RA, Trenkle A, Burroughs W. 1966: Influence of rumen protozoa on volatile acid production and ration digestibility in lambs. J Anim Sci 25: 1116-1122 <https://doi.org/10.2527/jas1966.2541116x>
25. Madeira MS, Cardoso C, Lopes PA, Coelho D, Afonso C 2017: Microalgae as feed ingredients for livestock production and meat quality: A review. Livest Sci 205: 111-121 <https://doi.org/10.1016/j.livsci.2017.09.020>
26. Males J, Purser D 1970: Relationship between rumen ammonia levels and the microbial population and volatile fatty acid proportions in faunated and defaunated sheep. Appl Microbiol 19: 485-490 <https://doi.org/10.1128/am.19.3.485-490.1970>
27. NRC 2016: National Academies of Sciences, Engineering, and Medicine. Nutrient Requirements of Beef Cattle: Eighth Revised Edition. Washington, DC: The National Academies Press, 494 p.
28. Newbold CJ, De La Fuente G, Belanche A, Ramos-Morales E, McEwan NR 2015: The role of ciliate protozoa in the rumen. Front microbiol 6: 164310 <https://doi.org/10.3389/fmicb.2015.01313>
29. Ogimoto K, Imai S 1981: Atlas of rumen microbiology. Japan Scientific Societies Press, 231 p.
30. Patra A, Stiverson J, Yu Z 2012: Effects of quillaja and yucca saponins on communities and select populations of rumen bacteria and archaea, and fermentation in vitro. J Appl Microbiol 113: 1329-1340 <https://doi.org/10.1111/j.1365-2672.2012.05440.x>
31. Ryckebosch E, Bruneel C, Muylaert K, Foubert I 2012: Microalgae as an alternative source of omega‐3 long chain polyunsaturated fatty acids. Lipid Technol 24: 128-130 <https://doi.org/10.1002/lite.201200197>
32. Van Soest PJ, Robertson J, Lewis B 1991: Methods of dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74: 3583-3597 <https://doi.org/10.3168/jds.S0022-0302(91)78551-2>
33. Van Soest PJ 1994: Nutritional ecology of the ruminant. Cornell university press, USA, 488 p.
34. Wild KJ, Steingaß H, Rodehutscord M 2019: Variability of in vitro ruminal fermentation and nutritional value of cell‐disrupted and nondisrupted microalgae for ruminants. GCB Bioenergy 11: 345-359 <https://doi.org/10.1111/gcbb.12539>
35. Williams AG, Coleman GS 1997: The Rumen Protozoa. In: Hobson PN, Stewart CS (Eds): The Rumen Microbial Ecosystem. Springer, Dordrecht, 740 p.
36. Wohlt J, Clark J, Blaisdell F 1976: Effect of sampling location, time, and method of concentration of ammonia nitrogen in rumen fluid. J Dairy Sci 59: 459-464 <https://doi.org/10.3168/jds.S0022-0302(76)84227-0>
37. Zheng Y, Xue S, Zhao Y, Li S 2020: Effect of cassava residue substituting for crushed maize on in vitro ruminal fermentation characteristics of dairy cows at mid-lactation. Animals 10: 893 <https://doi.org/10.3390/ani10050893>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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