Acta Vet. Brno 2025, 94: 193-202

https://doi.org/10.2754/avb202594030193

Studies on physical characteristics of phages against Escherichia coli and whole genome sequencing and analysis of a coliphage

Lavina1, Mudit Chandra1, Gurpreet Kaur1, Deepti Narang1, Anuj Tyagi2,3, Anil Kumar Arora1

1Guru Angad Dev Veterinary and Animal Sciences University, College of Veterinary Science, Department of Veterinary Microbiology, Ludhiana, Punjab, India
2College of Fisheries, Department of Aquatic Environment, Ludhiana, Punjab, India
3Guru Angad Dev Veterinary and Animal Sciences University, College of Fisheries, Department of Aquatic Environment, Ludhiana, Punjab, India

Received April 9, 2025
Accepted July 18, 2025

References

1. Abdelrahman F, Rezk N, Fayez MS, Abdelmoteleb M, Atteya R, Elhadidy M, El-Shibiny A 2022: Isolation, characterization, and genomic analysis of three novel E. coli bacteriophages that effectively infect E. coli O18. Microorganisms 10: 589 <https://doi.org/10.3390/microorganisms10030589>
2. Abedon ST 2009: Phage evolution and ecology. Adv Appl Microbiol 67: 1-45 <https://doi.org/10.1016/S0065-2164(08)01001-0>
3. Balcazar JL 2014: Bacteriophages as vehicles for antibiotic resistance genes in the environment. PLoS Pathog 10: e1004219 <https://doi.org/10.1371/journal.ppat.1004219>
4. Chandra M, Thakur S, Narang D 2011: Isolation of a bacteriophage against Salmonella Dublin and determination of its physical resistance under varied in vitro conditions. Afr J Microbiol Res 5: 2044-2047
5. Chinnadurai L, Eswaramoorthy T, Paramachandran A, Paul S, Rathy R, Arumugaperumal A, Sivasubramaniam S, Thavasimuthu C 2018: Draft genome sequence of Escherichia coli phage CMSTMSU, isolated from shrimp farm effluent water. Microbiol Resour Announc 7: e01034 <https://doi.org/10.1128/MRA.01034-18>
6. Cole D, Long SC, Sobsey MD 2003: Evaluation of F+ RNA and DNA coliphages as source-specific indicators of fecal contamination in surface waters. Appl Environ Microbiol 69: 6507-6514 <https://doi.org/10.1128/AEM.69.11.6507-6514.2003>
7. Davies-Colley RJ, Craggs RJ, Park J, Sukias JPS, Nagels JW, Stott R 2005: Virus removal in a pilot-scale ‘advanced’ pond system as indicated by somatic and F-RNA bacteriophages. Water Sci Technol 51: 107-110 <https://doi.org/10.2166/wst.2005.0440>
8. De Paepe M, Taddei F 2006: Viruses’ life history: towards a mechanistic basis of a trade-off between survival and reproduction among phages. PLoS Biol 4: e193 <https://doi.org/10.1371/journal.pbio.0040193>
9. Ewert DL, Paynter MJ 1980: Enumeration of bacteriophages and host bacteria in sewage and the activated-sludge treatment process. Appl Environ Microbiol 39: 576-583 <https://doi.org/10.1128/aem.39.3.576-583.1980>
10. Feiner R, Argov T, Rabinovich L, Sigal N, Borovok I, Herskovits AA 2015: A new perspective on lysogeny: prophages as active regulatory switches of bacteria. Nat Rev Microbiol 13: 641-650 <https://doi.org/10.1038/nrmicro3527>
11. Grabow WOK 2002: Bacteriophages: update on application as models for viruses in water. Water SA 27: 251-268
12. Hargreaves KR, Kropinski AM, Clokie MR 2014: Bacteriophage behavioral ecology: How phages alter their bacterial host’s habits. Bacteriophage 4: e29866 <https://doi.org/10.4161/bact.29866>
13. Hatfull GF, Hendrix RW 2011: Bacteriophages and their genomes. Curr Opin Virol 1: 298-303 <https://doi.org/10.1016/j.coviro.2011.06.009>
14. Jofre J, Lucena F, Blanch AR, Muniesa M 2016: Coliphages as model organisms in the characterization and management of water resources. Water 8: 199 <https://doi.org/10.3390/w8050199>
15. Jones TH, Brassard J, Topp E, Wilkes G, Lapend DR 2017: Waterborne viruses and F-specific coliphages in mixed-use watersheds: microbial associations, host specificities, and affinities with environmental/land use factors. Appl Environ Microbiol 83: e02763-16
16. Jurczak-Kurek A, Gąsior T, Nejman-Faleńczyk B, Bloch S, Dydecka A, Topka G, Necel A, Jakubowska-Deredas M, Narajczyk M, Richert M, Mieszkowska A, Wróbel B, Węgrzyn G, Węgrzyn A 2016: Biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage. Sci Rep 6: 34338 <https://doi.org/10.1038/srep34338>
17. Lin JT, Kirst S, Cucić S, Klem A, She YM, Kropinski AM, Anany H 2022: Isolation, characterization, and genome analysis of a novel bacteriophage, Escherichia phage vB_EcoM-4HA13, representing a new phage genus in the novel phage family Chaseviridae. Viruses 14: 2356 <https://doi.org/10.3390/v14112356>
18. Megha PU, Murugan S, Harikumar PS 2017: Isolation and characterization of lytic coliphages from sewage water. J Pure Appl Microbiol 11: 559-565 <https://doi.org/10.22207/JPAM.11.1.73>
19. Mesquita MM, Stimson J, Chae GT, Tufenkji N, Ptacek CJ, Blowes DW, Emelko MB 2010: Optimal preparation and purification of PRD1-like bacteriophages for use in environmental fate and transport studies. Water Res 44: 1114-1125 <https://doi.org/10.1016/j.watres.2009.11.017>
20. Mishra AK, Kumar A, Kumaresan G, Dixit A, Chaturvedi V, Gangwar C, Sharma N 2022: Isolation and therapeutic efficacy of enteropathogenic E. coli phage cocktail against colibacillosis in neonatal goats. Indian J Exp Biol 60: 832-841
21. Moon K, Jeon JH, Kang I, Park KS, Lee K, Cha CJ, Lee SH, Cho JC 2020: Freshwater viral metagenome reveals novel and functional phage-borne antibiotic resistance genes. Microbiome 8: 75 <https://doi.org/10.1186/s40168-020-00863-4>
22. Olsen NS, Forero-Junco L, Kot W, Hansen LH 2020: Exploring the remarkable diversity of culturable Escherichia coli phages in the Danish wastewater environment. Viruses 12: 986 <https://doi.org/10.3390/v12090986>
23. Rai S, Tyagi A, Naveen BT, Kaur S, Singh N 2019: Isolation, genomic characterization and stability study of narrow-host ra nge Aeromonas hydrophila lytic bacteriophage. J Exp Zoo India 22: 1075-1082
24. Ullah A, Qamash T, Khan FA, Sultan A, Ahmad S, Abbas M, Khattak MAK, Begum N, Din SU, Jamil J, Kalsoom 2021: Characterization of a coliphage AS1 isolated from sewage effluent in Pakistan. Braz J Biol 82: e240943 <https://doi.org/10.1590/1519-6984.240943>
25. Wommack KE 1999: Effects of sunlight on bacteriophage viability and structure. Appl Environ Microbiol 662: 1336-1341
26. Wommack KE, Colwell RR 2000: Virioplankton: viruses in aquatic ecosystems. Microbiol Mol Biol Rev 64: 69-114 <https://doi.org/10.1128/MMBR.64.1.69-114.2000>
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