Acta Vet. Brno 2025, 94: 223-230

https://doi.org/10.2754/avb202594030223

Assessment of acute stress in alpacas (Vicugna pacos): A pilot study using salivary cortisol and infrared thermography

Monika Šebánková, Gabriela Kadlecová, Eva Voslářová, Vladimír Večerek

University of Veterinary Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Protection and Welfare and Veterinary Public Health, Brno, Czech Republic

Received June 25, 2025
Accepted July 21, 2025

References

1. Abdel-Hamid TM, Dawod A 2020: Breed effects on growth performance, blood parameters and the levels of metabolic hormones in rabbits under heat stress in Egypt. Zagazig Vet J 48: 284-295 <https://doi.org/10.21608/zvjz.2020.28446.1107>
2. Anderson DE, Grubb T, Silveira F 1999: The effect of short duration transportation on serum cortisol response in alpacas (Llama pacos). Vet J 157: 189-191 <https://doi.org/10.1053/tvjl.1998.0270>
3. Andrade DV 2015: Thermal windows and heat exchange. Temperature 2: 451-451 <https://doi.org/10.1080/23328940.2015.1040945>
4. Arias N, Requena M, Palme R 2013: Measuring faecal glucocorticoid metabolites as a non-invasive tool for monitoring adrenocortical activity in South American camelids. Anim Welf 22: 25-31 <https://doi.org/10.7120/09627286.22.1.025>
5. Bartolomé E, Azcona F, Cañete-Aranda M, Perdomo-González DI, Ribes-Pons J, Terán EM 2019: Testing eye temperature assessed with infrared thermography to evaluate stress in meat goats raised in a semi-intensive farming system: a pilot study. Arch Anim Breed 62: 199-204 <https://doi.org/10.5194/aab-62-199-2019>
6. Blessing WW 2003: Lower brainstem pathways regulating sympathetically mediated changes in cutaneous blood flow. Cell Mol Neurobiol 23: 527-538 <https://doi.org/10.1023/A:1025020029037>
7. Cabezas S, Blas J, Marchant TA, Moreno S 2007: Physiological stress levels predict survival probabilities in wild rabbits. Horm Behav 51: 313-320 <https://doi.org/10.1016/j.yhbeh.2006.11.004>
8. Castilla-Castaño E, Herman N, Martinelli E, Lecru LA, Pressanti C, Schelcher F, Cadiergues MC 2021: Treatment of sarcoptic and chorioptic mange in an alpaca (Vicugna pacos) herd with a combination of topical amitraz and subcutaneous ivermectin. New Zeal Vet J 69: 121-126 <https://doi.org/10.1080/00480169.2020.1808544>
9. Chotard H, Ioannou S, Davila-Ross M 2018: Infrared thermal imaging: Positive and negative emotions modify the skin temperatures of monkey and ape faces. PLOS ONE 13: e0194527
10. Cook NJ 2012: Review: Minimally invasive sampling media and the measurement of corticosteroids as biomarkers of stress in animals. Can J Anim Sci 92: 227-259 <https://doi.org/10.4141/cjas2012-045>
11. de Lima V, Piles M, Rafel O, López-Béjar M, Ramón J, Velarde A, Dalmau A 2013: Use of infrared thermography to assess the influence of high environmental temperature on rabbits. Res Vet Sci 95: 802-810 <https://doi.org/10.1016/j.rvsc.2013.04.012>
12. Fan R, Gu Z, Guang X, Marín JC, Varas V, González BA, Dong C 2020: Genomic analysis of the domestication and post-Spanish conquest evolution of the llama and alpaca. Genome Biol 21: 1-26 <https://doi.org/10.1186/s13059-020-02080-6>
13. Fell LR, Shutt DA, Bentley CJ 1985: Development of a salivary cortisol method for detecting changes in plasma “free” cortisol arising from acute stress in sheep. Aust Vet J 62: 403-406 <https://doi.org/10.1111/j.1751-0813.1985.tb14120.x>
14. Fleischmann T, Siewert C, Staszyk C, Schulze M, Stadler P, Seifert H 2009: Thermal imaging as an aid to the diagnosis of pain in horses – first results. In: Dössel O, Schlegel WC (Eds): World Congress on Medical Physics and Biomedical Engineering, September 7-12, 2009, Munich, Germany, IFMBE Proceedings. Berlin: Springer, pp. 277-280
15. Gerken M 2010: Relationships between integumental characteristics and thermoregulation in South American camelids. Animal 4: 1451-1459 <https://doi.org/10.1017/S1751731109991443>
16. Goñalons GLM 2008: Camelids in ancient Andean societies: A review of the zooarchaeological evidence. Quaternary International 185: 59-68 <https://doi.org/10.1016/j.quaint.2007.05.022>
17. Herborn KA, Graves JL, Jerem P, Evans NP, Nager R, McCafferty DJ, McKeegan DEF 2015: Skin temperature reveals the intensity of acute stress. Physiol Behav 152: 225-230 <https://doi.org/10.1016/j.physbeh.2015.09.032>
18. Hostovská L, Hostovský M, Nenadović K, Voslářová E, Večerek V 2024: Effect of different types of exercise on salivary biochemical indices in the horse. Acta Vet Brno 93: 281-287 <https://doi.org/10.2754/avb202493030281>
19. Jimoh OA, Ewuola EO 2018: Thermophysiological traits in four exotic breeds of rabbit at least temperature-humidity index in humid tropics. J Basic Appl Zool 79: 18 <https://doi.org/10.1186/s41936-018-0031-9>
20. Kapustka J, Garbiec A 2022: Alpacas in Poland: health, welfare, and anti-parasitic prophylaxis. Med Weter 78: 68-73
21. Kadlecová G, Šebánková M, Voslářová E, Večerek V 2024: The use of infrared thermography in the evaluation of acute stress in three breeds of domestic rabbits during tattooing. Acta Vet Brno 93: 439-446 <https://doi.org/10.2754/avb202493040439>
22. Kotianová L, Lakomá T, Vacušková Z, Vacuška D, Večerek V, Voslářová E 2025: Behavioural and physiological response to routine husbandry procedures in Wallachian sheep. Acta Vet Brno 94: 35-42 <https://doi.org/10.2754/avb202594010035>
23. Lowe G, Sutherland M, Waas J, Schaefer A, Cox N, Stewart M 2019: Infrared thermography—a non-invasive method of measuring respiration rate in calves. Animals 9: 535 <https://doi.org/10.3390/ani9080535>
24. Majchrzak YN, Mastromonaco GF, Korver WBG 2015: Use of salivary cortisol to evaluate the influence of rides in dromedary camels. Gen Comp Endocrinol 211: 123-130 <https://doi.org/10.1016/j.ygcen.2014.11.007>
25. Marketon JIW, Glaser R 2008: Stress hormones and immune function. Cell Immunol 252: 16-26 <https://doi.org/10.1016/j.cellimm.2007.09.006>
26. McMullen C 2008: Romancing the alpaca: Passionate consumption, collection, and companionship. J Bus Res 61: 502-508 <https://doi.org/10.1016/j.jbusres.2007.07.023>
27. Möstl E, Palme R 2002: Hormones as indicators of stress. Domest Anim Endocrinol 23: 67-74 <https://doi.org/10.1016/S0739-7240(02)00146-7>
28. Pollard JC, Littlejohn RP 1995: Effects of social isolation and restraint on heart rate and behaviour of alpacas. Appl Anim Behav Sci 45: 165-174 <https://doi.org/10.1016/0168-1591(95)00588-J>
29. Prágai A, Kovács A 2020: Stress of alpacas caused by shearing in Hungary. Bulg J Agric Sci 26: 207-212
30. Raggi LA, Crossley J, Coppia S, Ferrando G 1994: Características fisiológicas de la alpaca (Lama pacos) sometida a manejo extensivo en el altiplano chileno. Arch Zootec 43: 201-206
31. Rosenmeier JG, Strathe AB, Andersen PH 2012: Evaluation of coronary band temperatures in healthy horses. Am J Vet Res 73: 719-723 <https://doi.org/10.2460/ajvr.73.5.719>
32. Sheriff MJ, Dantzer B, Delehanty B, Palme R, Boonstra R 2011: Measuring stress in wildlife: techniques for quantifying glucocorticoids. Oecologia 166: 869-887 <https://doi.org/10.1007/s00442-011-1943-y>
33. Švestková M, Pištěková V, Takáčová D, Večerek V, Voslářová E 2024a: Analysis of the major deficiencies detected during welfare inspections of farm animals in the Czech Republic. Acta Vet Brno 93: 45-57 <https://doi.org/10.2754/avb202493010045>
34. Švestková M, Pištěková V, Takáčová D, Večerek V, Voslářová E 2024b: Deficiencies in livestock holdings with respect to animal welfare identified as part of cross-compliance checks completed in 2016–2020 in the Czech Republic. Acta Vet Brno 93: 239-250 <https://doi.org/10.2754/avb202493020239>
35. Travain T, Colombo ES, Heinzl E, Bellucci D, Prato Previde E, Valsecchi P 2015: Hot dogs: Thermography in the assessment of stress in dogs (Canis familiaris)-A pilot study. J Vet Behav 10: 17-23 <https://doi.org/10.1016/j.jveb.2014.11.003>
36. Travain T, Valsecchi P 2021: Infrared thermography in the study of animals’ emotional responses: A critical review. Animals 11: 2510 <https://doi.org/10.3390/ani11092510>
37. Tynes VV 2010: Behavior of exotic pets. Wiley-Blackwell Publishing, Chichester, West Sussex, 256 p.
38. Valera M, Bartolomé E, Sánchez MJ, Molina A, Cook N, Schaefer A 2012: Changes in eye temperature and stress assessment in horses during show jumping competitions. J Equine Vet Sci 32: 827-830 <https://doi.org/10.1016/j.jevs.2012.03.005>
39. Van Saun RJ 2006: Nutritional diseases of South American camelids. Small Rumin Res 61: 153-164 <https://doi.org/10.1016/j.smallrumres.2005.07.007>
40. Vianna DML, Carrive P 2005: Changes in cutaneous and body temperature during and after conditioned fear to context in the rat. Eur J Neurosci 21: 2505-2512 <https://doi.org/10.1111/j.1460-9568.2005.04073.x>
41. Waiblinger S, Hajek F, Lambacher B, Wittek T 2020: Effects of the method of restraint for shearing on behaviour and heart rate variability in alpacas. Appl Anim Behav Sci 223: 104918 <https://doi.org/10.1016/j.applanim.2019.104918>
42. Whitehead C 2013: Diseases in camelids 1. Common presentations. In Practice 35: 317-324 <https://doi.org/10.1136/inp.f3641>
43. Wittek T, Salaberger T, Palme R, Becker S, Hajek F, Lambacher B, Waiblinger S 2017: Clinical parameters and adrenocortical activity to assess stress responses of alpacas using different methods of restraint either alone or with shearing. Vet Rec 180: 568-568 <https://doi.org/10.1136/vr.104232>
44. Yarnell K, Hall C, Billett E 2013: An assessment of the aversive nature of an animal management procedure (clipping) using behavioral and physiological measures. Physiol Behav 118: 32-39 <https://doi.org/10.1016/j.physbeh.2013.05.013>
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