Acta Vet. Brno 2025, 94: 251-259
Raman spectroscopy and NIR as a method of comparing monofloral and honeydew honeys
Honey is one of the most commonly adulterated foods. This study therefore focused on innovative possibilities of identifying the botanical origin of honey using Raman spectroscopy and Fourier transform near-infrared spectroscopy (FT-NIR), in order to test their potential use as an alternative to physicochemical analysis and melissopalynology methods. Both mentioned non-destructive methods were used for discrimination and characterization of the honey species. Forty-seven samples of flower and honeydew honey obtained from hobby beekeepers from different regions of the Czech Republic were analysed in the period between 2019 and 2021. Among the floral honeys, poly- as well as monofloral honeys were selected (rapeseed, clover, acacia, phacelia, fruit tree honey). The FT-NIR analysis results confirmed significant differences (P < 0.05) between the specific-species honey samples for wavenumbers of 5,624, 5,171, 4,780, 4,391 cm-1 (P < 0.05). Raman spectroscopy confirmed significant differences between multiple wavenumbers, with classification into the most classes confirmed for wavenumbers of 308, 606, 613, 620, 999 and 1,013 cm-1 (P < 0.05). However, neither method allowed classification for all honey species, thus FT-NIR and Raman spectra were compared by linear discriminant analysis (LDA), which confirmed a high correct classification rate (CCR) of 99.38%, and 91.30% in the case of cross-validation. In the case of rapeseed honey, the lowest CCR (96.15%) and cross-validation (76.92%) values were confirmed. It was confirmed that the FT-NIR method in combination with Raman spectroscopy can be used to identify individual honey species with a high degree of reliability.
Keywords
Foodstuff adulteration, non-destructive analysis, wavenumber.
Funding
The study was supported by the Applied Research Programme of the Ministry of Agriculture for the 2017-2025 period [Project No. QK1920344, ‘ZEMĚ’ (‘The Land’)].
