Acta Vet. Brno 2026, 95: 63-69

https://doi.org/10.2754/avb202695010063

The virucidal effect of 405 nm visible light on selected viruses

Lucie Janíček Hrubá1, Veronika Vojtkovská2, Vladimír Celer1

1University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Department of Infectious Diseases and Microbiology, Brno, Czech Republic
2University of Veterinary Sciences Brno, Faculty of Veterinary Medicine, Department of Animal Protection and Welfare and Veterinary Public Health, Brno, Czech Republic

Received September 16, 2025
Accepted March 2, 2026

References

1. Ash C, Dubec M, Donne K, Bashford T 2017: Effect of wavelength and beam width on penetration in light-tissue interaction using computational methods. Lasers Med Sci 32: 1909-1918 <https://doi.org/10.1007/s10103-017-2317-4>
2. Bumah VV, Aboualizadeh E, Masson-Meyers DS, Eells JT, Enwemeka CS, Hirschmugl CJ 2017: Spectrally resolved infrared microscopy and chemometric tools to reveal the interaction between blue light (470 nm) and methicillin-resistant Staphylococcus aureus. J Photochem Photobiol B 167: 150-157 <https://doi.org/10.1016/j.jphotobiol.2016.12.030>
3. Calatayud O, Esperón F, Cleaveland S, Biek J, Eblate E, Neves E, Lembo T, Lankerster F 2019: Carnivore parvovirus ecology in the Serengeti ecosystem: vaccine strains circulating and new host species identified. J Virol 93: e02220-18 <https://doi.org/10.1128/JVI.02220-18>
4. Gardner A, Ghosh S, Dunowska M, Brightwell G 2021: Virucidal efficacy of blue LED and far-UVC light disinfection against feline infectious peritonitis virus as a model for SARS-CoV-2. Viruses 13: 1436 <https://doi.org/10.3390/v13081436>
5. Girard PM, Francesconi S, Pozzebon M, Graindorge D, Rochette P, Drouin R 2011: UVA-induced damage to DNA and proteins: Direct versus indirect photochemical processes. J Phys Conf Ser 261: 012002 <https://doi.org/10.1088/1742-6596/261/1/012002>
6. Gordon JC, Angrick EJ 1986: Canine parvovirus: environmental effects on infectivity. Am J Vet Res 47: 1464-1467 <https://doi.org/10.2460/ajvr.1986.47.07.1464>
7. Hadi J, Dunowska M, Wu S, Brightwell G 2020: Control measures for SARS-CoV-2: a review on light-based inactivation of single-stranded RNA viruses. Pathogens 9: 737 <https://doi.org/10.3390/pathogens9090737>
8. Helps CR, Lait P, Tasker S, Harbour DA 2002: Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR. J Virol Methods 106: 241-244 <https://doi.org/10.1016/S0166-0934(02)00167-2>
9. Ho DT, Kim A, Kim N, Roh HJ, Chun W-K, Lee Y, Kim D-H 2020: Effect of blue light emitting diode on viral hemorrhagic septicemia in olive flounder (Paralichthys olivaceus). Aquaculture 521: 735019 <https://doi.org/10.1016/j.aquaculture.2020.735019>
10. Hessling M, Lau B, Vatter P 2022: Review of virus inactivation by visible light. Photonics 9: 113 <https://doi.org/10.3390/photonics9020113>
11. Maclean M, McKenzie K, Anderson JG, Gettinby G, MacGregor SJ 2014: 405 nm light technology for the inactivation of pathogens and its potential role for environmental disinfection and infection control. J Hosp Infect 88: 1-11 <https://doi.org/10.1016/j.jhin.2014.06.004>
12. Plavskii VY, Mikulich AV, Tretyakova AI, Leusenka IA, Plavskaya LG, Kazyuchits OA, Dobysh II, Krasnenkova TP 2018: Porphyrins and flavins as endogenous acceptors of optical radiation of blue spectral region determining photoinactivation of microbial cells. J Photochem Photobiol B 183: 172-183 <https://doi.org/10.1016/j.jphotobiol.2018.04.021>
13. Radford AD, Coyne KP, Dawson S, Porter CJ, Gaskell, RM 2007: Feline calicivirus. Vet Res 38: 319-335 <https://doi.org/10.1051/vetres:2006056>
14. Rathnasinghe R, Jangra S, Miorin L, Schotsaert M, Yahnke C, Garcίa-Sastre A 2021: The virucidal effects of 405 nm visible light on SARS-CoV-2 and influenza A virus. Sci Rep 11: 1-10
15. Reed LJ, Muench H 1938: A simple method of estimating fifty per cent endpoints. Am J Hyg 27: 493-497
16. Sliney D 2013: Balancing the risk of eye irritation from UV-C with infection from bioaerosols. Photochem Photobiol 89: 770-776 <https://doi.org/10.1111/php.12093>
17. Tomb RM, Maclean M, Herron PR, Hoskisson PA, MacGregor SJ, Anderson JG 2014: Inactivation of Streptomyces phage ɸC31 by 405 nm light: Requirement for exogenous photosensitizers? Bacteriophage 4: e32129 <https://doi.org/10.4161/bact.32129>
18. Tomb RM, Maclean M, Coia JE, Graham E, McDonald M, Atreya CD, MacGregor SJ, Anderson JG 2017: New proof-of-concept in viral inactivation: Virucidal efficacy of 405 nm light against feline calicivirus as a model for norovirus decontamination. Food Environ Virol 9: 159-167 <https://doi.org/10.1007/s12560-016-9275-z>
19. Tomb RM, White TA, Coia JE, Anderson JG, MacGregor SJ, Maclean M 2018: Review of the comparative susceptibility of microbial species to photoinactivation using 380–480 nm violet-blue light. Photochem Photobiol 94: 445-458 <https://doi.org/10.1111/php.12883>
20. Wilhelm S, Truyen U 2006: Real-time reverse transcription polymerase chain reaction assay to detect a broad range of feline calicivirus isolates. J Virol Methods 133: 105-108 <https://doi.org/10.1016/j.jviromet.2005.10.011>
21. Wu J, Hou W, Cao B, Zuo T, Xue C, Leung AW, Xu C, Tang Q-J 2015: Virucidal efficacy of treatment with photodynamically activated curcumin on murine norovirus bio-accumulated in oysters. Photodiagnosis Photodyn Ther 12: 385-392 <https://doi.org/10.1016/j.pdpdt.2015.06.005>
22. Zhang Y, Zhu Y, Chen J, Wang Y, Sherwood ME, Murray CK, Vrahas MS, Hooper DC, Hamblin MR, Dai T 2016: Antimicrobial blue light inactivation of Candida albicans: in vitro and in vivo studies. Virulence 7: 536-545 <https://doi.org/10.1080/21505594.2016.1155015>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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