Acta Vet. Brno 2026, 95: 71-84
https://doi.org/10.2754/avb202695010071
Molecular surveillance of antimicrobial resistance in Bacillus cereus sensu lato: A proposed conceptual framework in the One Health context
References
1. MNS, Zayda MG, Maung AT, El-Telbany M, Mohammadi TN, Lwin SZC, Linn KZ, Wang C, Yuan L, Masuda Y, Honjoh KI, Miyamoto T 2024: Genetic characterization, antibiotic resistance, and virulence genes profiling of Bacillus cereus strains from various foods in Japan. Antibiotics 13: 774
<https://doi.org/10.3390/antibiotics13080774>
2. Y, Jensen LB, Givskov M, Roberts MC 2002: The identification of a tetracycline resistance gene tet(M), on a Tn 916-like transposon, in the Bacillus cereus group. FEMS Microbiol Lett 214: 251-256
<https://doi.org/10.1016/S0378-1097(02)00883-2>
3. AM, Eid HM, Alghamdi S, Ghabban H, Alatawy R, Almanzalawi EA, El-Tarabili RM 2024: Meat and meat products as potential sources of emerging MDR Bacillus cereus: groEL gene sequencing, toxigenic and antimicrobial resistance. BMC Microbiol 24: 50
<https://doi.org/10.1186/s12866-024-03204-9>
4. R, Krakat N 2016: Detection of multiple resistances, biofilm formation and conjugative transfer of Bacillus cereus from contaminated soils. Curr Microbiol 72: 321-328
<https://doi.org/10.1007/s00284-015-0952-1>
5. MH, Schellhase M, Ehrentreich-Förster E, Bier F, Witte W, Nübel U 2007: DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus. Mol Cell Probes 21: 152-160
<https://doi.org/10.1016/j.mcp.2006.10.002>
6. SD, Walsh KA, Gould LH 2013: Foodborne disease outbreaks caused by Bacillus cereus, Clostridium perfringens, and Staphylococcus aureus—United States, 1998–2008. Clin Infect Dis 57: 425-433
<https://doi.org/10.1093/cid/cit244>
7. A, Capozzi L, Monno MR, Del Sambro L, Manzulli V, Pesole G, Loconsole D, Parisi A 2021: Characterization of Bacillus cereus group isolates from human bacteremia by whole-genome sequencing. Front Microbiol 11: 599524
<https://doi.org/10.3389/fmicb.2020.599524>
8. B, Fraiture M-A, Huwaert A, Van Nieuwenhuysen T, Jacobs B, Van Hoorde K, De Keersmaecker SCJ, Roosens NHC, Vanneste K 2023: Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing. Front Microbiol 14: 1173594
<https://doi.org/10.3389/fmicb.2023.1173594>
9. PJ, Garcia-Graells C, Fux F, Botteldoorn N, Mattheus W, Wuyts V, De Keersmaecker S, Dierick K, Bertrand S 2016: Development of a Luminex xTAG assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria. J Antimicrob Chemother 71: 2479-2483
<https://doi.org/10.1093/jac/dkw201>
10. X, Lin Y, Brennan C, Cao J, Shang Y 2023: Antibiotic resistance of Bacillus cereus in plant foods and edible wild mushrooms in a province. Microorganisms 11: 2948
<https://doi.org/10.3390/microorganisms11122948>
11. K, Kameník J, Bogdanovičová K, Křepelová S, Strejček J, Haruštiaková D 2022: Microbial contamination and occurrence of Bacillus cereus sensu lato, Staphylococcus aureus, and Escherichia coli on food handlers’ hands in mass catering: Comparison of the glove juice and swab methods. Food Control 133: 108567
<https://doi.org/10.1016/j.foodcont.2021.108567>
12. M, Kameník J, Čutová M, Dorotíková K, Králová M, Šedo O 2025: Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues. Acta Vet Brno 94: 317-327
<https://doi.org/10.2754/avb202594040317>
13. S, Yu Y, Guo Y, Lu D, Li N, Liu Z, Liang J, Jiang Y, Wang S, Fu P, Liu J, Liu H 2023: Epidemiological evaluation of Bacillus cereus-induced foodborne outbreaks—China, 2010–2020. China CDC Wkly 5: 737-741
<https://doi.org/10.46234/ccdcw2023.140>
14. ECDC 2024: The European Union One Health 2023 Zoonoses report. EFSA J 22: e9106
15. N, Kallassy Awad M, Mahillon J 2019: Diversity of Bacillus cereus sensu lato mobilome. BMC Genomics 20: 436
<https://doi.org/10.1186/s12864-019-5764-4>
16. D, Bianco A, Manzulli V, Castellana S, Parisi A, Caruso M, Fraccalvieri R, Serrecchia L, Rondinone V, Pace L, Fasanella A, Vetritto V, Difato LM, Cipolletta D, Iatarola M, Galante D 2024: Antimicrobial and phylogenomic characterization of Bacillus cereus group strains isolated from different food sources in Italy. Antibiotics 13: 898
<https://doi.org/10.3390/antibiotics13090898>
17. G, Schneider C, Igbinosa EO, Kabisch J, Brinks E, Becker B, Stoll DA, Cho G-S, Huch M, Franz CMAP 2019: Antibiotics resistance and toxin profiles of Bacillus cereus-group isolates from fresh vegetables from German retail markets. BMC Microbiol 19: 250
<https://doi.org/10.1186/s12866-019-1632-2>
18. R, Bianco A, Difato LM, Capozzi L, Del Sambro L, Simone D, Catanzariti R, Caruso M, Galante D, Normanno G, Palazzo L, Tempesta M, Parisi A 2022: Toxigenic genes, pathogenic potential and antimicrobial resistance of Bacillus cereus group isolated from ice cream and characterized by whole genome sequencing. Foods 11: 2480
<https://doi.org/10.3390/foods11162480>
19. H, Thanh MD, Krause G, Appel B, Mader A 2018: Quantification and differentiation of Bacillus cereus group species in spices and herbs by real-time PCR. Food Control 83: 99-108
<https://doi.org/10.1016/j.foodcont.2016.11.028>
20. BSP, Ferrari RG, Panzenhagen P, de Jesus ACS, Conte-Junior CA 2021: Antimicrobial resistance gene detection methods for bacteria in animal-based foods: a brief review of highlights and advantages. Microorganisms 9: 923
<https://doi.org/10.3390/microorganisms9050923>
21. P, Mao D, Luo Y, Wang L, Xu B, Xu L 2012: Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment. Water Res 46: 2355-2364
<https://doi.org/10.1016/j.watres.2012.02.004>
22. T, Ding Y, Wu Q, Wang J, Zhang J, Yu S, Yu P, Liu C, Kong L, Feng Z, Chen M, Wu S, Zeng H, Wu H 2018: Prevalence, virulence genes, antimicrobial susceptibility, and genetic diversity of Bacillus cereus isolated from pasteurized milk in China. Front Microbiol 9: 533
<https://doi.org/10.3389/fmicb.2018.00533>
23. T, Ochoa-Sánchez LE, Baquero F, Martínez JL 2021: Antibiotic resistance: time of synthesis in a post-genomic age. Comput Struct Biotechnol J 19: 3110-3124
<https://doi.org/10.1016/j.csbj.2021.05.034>
24. H, Pohl S, Navarro F, Miro E, Jiménez G, Blanch AR, Harwood CR 2017: The identification of intrinsic chloramphenicol and tetracycline resistance genes in members of the Bacillus cereus group (sensu lato). Front Microbiol 7: 2122
<https://doi.org/10.3389/fmicb.2016.02122>
25. S, Ma AH, Tariq Z, Vega Prado I, Drobish I, Lee R, Yee R 2024: Rapid phenotypic and genotypic antimicrobial susceptibility testing approaches for use in the clinical laboratory. Antibiotics 13: 786
<https://doi.org/10.3390/antibiotics13080786>
26. J, Vasickova P, Nesvadbova M, Novotny J, Mati T, Kralik P 2021: MOL-PCR and xMAP technology: A multiplex system for fast detection of food- and waterborne viruses. J Mol Diagn 23: 765-776
<https://doi.org/10.1016/j.jmoldx.2021.03.005>
27. LB, Agersø Y, Sengeløv G 2002: Presence of erm genes among macrolide-resistant Gram-positive bacteria isolated from Danish farm soil. Environ Int 28: 487-491
<https://doi.org/10.1016/S0160-4120(02)00076-4>
28. N, Dietrich R, Granum PE, Märtlbauer E 2020: The Bacillus cereus food infection as multifactorial process. Toxins 12: 701
<https://doi.org/10.3390/toxins12110701>
29. C, Timmermans M, Fretin D, Wattiau P, Boland C 2023: An in-house 45-plex array for the detection of antimicrobial resistance genes in Gram-positive bacteria. Microbiologyopen 12: e1341
<https://doi.org/10.1002/mbo3.1341>
30. P, Ricchi M 2017: A basic guide to real time PCR in microbial diagnostics: definitions, parameters, and everything. Front Microbiol 8: 108
<https://doi.org/10.3389/fmicb.2017.00108>
31. N, Siddique A, Liu N, Teng L, Ed-Dra A, Yue M, Li Y 2025: Global epidemiology and health risks of Bacillus cereus infections: special focus on infant foods. Food Res Int 201: 115650
<https://doi.org/10.1016/j.foodres.2024.115650>
32. E, Tosello B, Schipani S, Gouriet F, Caspar JP, L’Hostis V, Zahar JR, Almasri MK, Baudoin JP, Eldin C 2025: Environmental investigation of 10 cases of nosocomial Bacillus cereus bacteraemia between 2018 and 2023. Antimicrob Resist Infect Control: 14: 62
<https://doi.org/10.1186/s13756-025-01586-7>
33. U, Ehling-Schulz M 2018: Bacillus cereus—a multifaceted opportunistic pathogen. Curr Clin Microbiol Rep 5: 120-125
<https://doi.org/10.1007/s40588-018-0095-9>
34. E, Sullivan E, Kovac J 2022: Comparative analysis of Bacillus cereus group isolates’ resistance using disk diffusion and broth microdilution and the correlation between antimicrobial resistance phenotypes and genotypes. Appl Environ Microbiol 88: e0230221
<https://doi.org/10.1128/aem.02302-21>
35. RS, Sonawane KD 2018: Insights into the antibiotic resistance and inhibition mechanism of aminoglycoside phosphotransferase from Bacillus cereus: in silico and in vitro perspective. J Cell Biochem 119: 9444-9461
<https://doi.org/10.1002/jcb.27261>
36. H, Azari R, Yousefi MH, Berizi E, Mazloomi SM, Hosseinzadeh S, Derakhshan Z, Ferrante M, Conti GO 2023: A systematic review and meta-analysis of the prevalence of Bacillus cereus in foods. Food Control 143: 109250
<https://doi.org/10.1016/j.foodcont.2022.109250>
37. N, Huvarova V, Hrdy J, Kasny M, Kralik P 2019: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay. Sci Rep 9: 2719
<https://doi.org/10.1038/s41598-019-40035-5>
38. C, Fernández J, Vázquez X, de Toro M, Ladero V, Fuster C, Rodicio R, Rodicio MR 2022: Detection of the optrA gene among polyclonal linezolid-susceptible isolates of Enterococcus faecalis recovered from community patients. Microb Drug Resist 28: 773-779
<https://doi.org/10.1089/mdr.2021.0402>
39. M, Rafi MA, Mishu ID, Mahmud Z 2025: Comprehensive genomic analysis reveals virulence and antibiotic resistance genes in a multidrug-resistant Bacillus cereus isolated from hospital wastewater in Bangladesh. Sci Rep 15: 22915
<https://doi.org/10.1038/s41598-025-06655-w>
40. PJ, Forstner P, Kittinger C 2024: Sliding motility of Bacillus cereus mediates vancomycin pseudo-resistance during antimicrobial susceptibility testing. J Antimicrob Chemother 79: 1628-1636
<https://doi.org/10.1093/jac/dkae156>
41. S, Zhang W, Du X-D, Krüger H, Feßler AT, Ma S, Zhu Y, Wu C, Shen J, Wang Y 2021: Mobile oxazolidinone resistance genes in Gram-positive and Gram-negative bacteria. Clin Microbiol Rev 34: e0018820
<https://doi.org/10.1128/CMR.00188-20>
42. J, Li PE, Gans J, Vuyisich M, Deshpande A, Wolinsky M, White PS 2010: Simultaneous pathogen detection and antibiotic resistance characterization using SNP-based multiplexed oligonucleotide ligation-PCR (MOL-PCR). Adv Exp Med Biol 680: 455-464
<https://doi.org/10.1007/978-1-4419-5913-3_51>
43. I, Kwiecień E, Jóźwiak-Piasecka K, Garbowska M, Binek M, Rzewuska M 2021: Antimicrobial susceptibility of lactic acid bacteria strains of potential use as feed additives – the basic safety and usefulness criterion. Front Vet Sci 8: 687071
<https://doi.org/10.3389/fvets.2021.687071>
44. M, Cousin VL, Tissieres P, Enault M, Morin L 2022: Multi-organ failure caused by lasagnas: a case report of Bacillus cereus food poisoning. Front Pediatr 10: 978250
<https://doi.org/10.3389/fped.2022.978250>
45. E, Stella S, Celandroni F, Mazzantini D, Bernardi C, Ghelardi E 2022: Bacillus cereus in dairy products and production plants. Foods 11: 2572
<https://doi.org/10.3390/foods11172572>
46. Baghbadorani S, Rahimi E, Shakerian A 2023: Investigation of virulence and antibiotic-resistance of Bacillus cereus isolated from various spices. Can J Infect Dis Med Microbiol 2023: 8390778
<https://doi.org/10.1155/2023/8390778>
47. N, Laflamme C, Ho J, Duchaine C 2008: Evaluation of the plasmid copy number in B. cereus spores, during germination, bacterial growth and sporulation using real-time PCR. Plasmid 60: 118-124
<https://doi.org/10.1016/j.plasmid.2008.05.001>
48. AE, Tillman AR, Hedberg C, Bruce BB, Batz M, Seys SA, Dewey-Mattia D, Bazaco MC, Walter ES 2022: Foodborne illness outbreaks reported to national surveillance, United States, 2009–2018. Emerg Infect Dis 28: 1117-1127
<https://doi.org/10.3201/eid2806.211555>
49. V, Mattheus W, Roosens NH, Marchal K, Bertrand S, De Keersmaecker SC 2015: A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium. Appl Microbiol Biotechnol 99: 8137-8149
<https://doi.org/10.1007/s00253-015-6831-7>
50. D, Uskoković V, Wakil AM, Goni MD, Shamsuddin SH, Mustafa FH, Alfouzan WA, Alissa M, Alshengeti A, Almaghrabi RH, Fares MAA, Garout M, Al Kaabi NA, Alshehri AA, Ali HM, Rabaan AA, Aldubisi FA, Yean CY, Yusof NY 2023: Current and future technologies for the detection of antibiotic-resistant bacteria. Diagnostics 13: 3246
<https://doi.org/10.3390/diagnostics13203246>
51. C, Yu T 2019: Characterization and transfer of antimicrobial resistance in lactic acid bacteria from fermented dairy products in China. J Infect Dev Ctries 13: 137-148
<https://doi.org/10.3855/jidc.10765>
52. P, Yu S, Wang J, Guo H, Zhang Y, Liao X, Zhang J, Wu S, Gu Q, Xue L, Zeng H, Pang R, Lei T, Zhang J, Wu Q, Ding Y 2020: Corrigendum: Bacillus cereus isolated from vegetables in China: Incidence, genetic diversity, virulence genes, and antimicrobial resistance. Front Microbiol 11: 848
<https://doi.org/10.3389/fmicb.2020.00848>
53. Z, Cui C, Li X, Yan J, Sun E, Wang C, Guo H, Hao Y 2023: Prevalence, antimicrobial susceptibility, and antibiotic resistance gene transfer of Bacillus strains isolated from pasteurized milk. J Dairy Sci 106: 75-83
<https://doi.org/10.3168/jds.2022-22199>
54. J, Guan Z, Cao S, Peng D, Ruan L, Jiang D, Sun M 2015: Plasmids are vectors for redundant chromosomal genes in the Bacillus cereus group. BMC Genomics 16: 6
<https://doi.org/10.1186/s12864-014-1206-5>
55. Z, Ye L, Xiong W, Hu Q, Chen K, Sun R, Chen S 2024: Prevalence and genomic characterization of the Bacillus cereus group strains contamination in food products in Southern China. Sci Total Environ 921: 170903
<https://doi.org/10.1016/j.scitotenv.2024.170903>
