Acta Vet. Brno 2026, 95: 99-106

https://doi.org/10.2754/avb202695010099

Does the application of a protective microbial culture influence the quality of cooked fish sausages?

Miroslav Jůzl1, Libor Kalhotka1, Veronika Švehlová1, Lukáš Harabiš2, Jan Mareš2, Šárka Nedomová1, Petr Kouřil1, Romana Veselá1, Markéta Janík Piechowiczová1, Aneta Kocandová1, Jan Slováček1

1Mendel University in Brno, Faculty of AgriSciences, Department of Food Technology, Brno, Czech Republic
2Mendel University in Brno, Faculty of AgriSciences, Department of Zoology, Fisheries, Hydrobiology and Apiculture, Brno, Czech Republic

Received October 12, 2025
Accepted March 2, 2026

In this study, meat from freshly slaughtered African sharptooth catfish (Clarias gariepinus) was used to assess whether the technological application of a protective microbial culture (PMC) could influence the quality and hygienic evaluation of a model fish meat product. The fillets were processed into cooked fish sausages prepared either from freshly killed fish (F – fresh meat) or from meat stored for 3 days at 2 °C (S – stored meat). Each of these two variants was further divided into subgroups according to the application of a commercially available protective culture (Pediococcus acidilactici, P) or without it (control, C), resulting in a 2 × 2 factorial design. Significant differences (P < 0.05) were observed only in texture indicators of sausages prepared from stored fish meat. However, the application of PMC had no significant effect (P > 0.05) on the microbiological or overall quality indicators of fish meat and cooked sausages.

Funding

This research was supported by the project QK23020047 ‘Verifying the possibilities of proving the use of protective cultures in the production of food of animal origin’, funded under the applied research programme of the Ministry of Agriculture of the Czech Republic for the period 2017–2025 (ZEMĚ, sub-programme 2 – Support for State Policy in the Agrarian Sector). The authors gratefully acknowledge the technical assistance of the staff of the Food Technology Pilot Plant (CZ 22067) and the Department of Zoology, Fisheries, Hydrobiology and Apiculture at Mendel University in Brno.

References

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