Genetic diversity and phylogenetic position of Manavli goat based on mitochondrial DNA (D-loop) region

Akbaş, Aykut Asım; Doğan, Müge; Saatci, Mustafa; Elmaz, Özkan; Yazici, Can Metin

doi:10.2754/avb202695010031

Acta Veterinaria > Archive > 2026, Vol. 95 > Issue 1 > Genetic diversity and phylogenetic…

Acta Vet. Brno 2026, 95: 31-38

https://doi.org/10.2754/avb202695010031

Genetic diversity and phylogenetic position of Manavli goat based on mitochondrial DNA (D-loop) region

Aykut Asım Akbaş¹

, Müge Doğan²

, Mustafa Saatci³

, Özkan Elmaz¹

, Can Metin Yazici¹

¹Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Animal Science, Burdur, Türkiye
²Konya Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Konya, Türkiye
³Muğla Sıtkı Koçman University, Fethiye Faculty of Agriculture, Department of Animal Science, Muğla, Türkiye

Received November 3, 2025
Accepted March 2, 2026

This study investigates the maternal genetic diversity and phylogeographic relationships of the Manavli goat population, a native genotype of Anatolia, using mitochondrial DNA (mtDNA) D-loop sequences. A total of 17 Manavli goats were analysed and compared with reference sequences from other Anatolian goat breeds as well as several global goat breeds. Sequence analyses revealed 30 polymorphic sites defining eight different haplotypes within the Manavli goat population. The haplotype diversity (Hd = 0.824) and nucleotide diversity (π = 0.0128) values indicate a moderate level of genetic variation. Results of the analysis of molecular variance (AMOVA) indicated that approximately 10.18% of the total genetic variation was due to differences among populations (Fst = 0.1018, P < 0.01), while 89.82% of the variation occurred within populations. In the unweighted pair group method with arithmetic mean (UPGMA) dendrogram constructed with other indigenous Anatolian goat breeds, clustering based on genetic distance revealed a closer genetic relationship between the Manavli and Ankara goats. All phylogenetic and haplotype network analyses demonstrated that the Manavli goat population predominantly clusters within the G and A haplogroups, possessing unique maternal lineages. These findings emphasize the genetic distinctiveness of the Manavli goat and highlight its importance as a local genetic resource for conservation and sustainable breeding programs in Anatolia. To further validate and explore the observed genetic data, it is strongly recommended that future studies include larger sample sizes.