Acta Vet. Brno 2026, 95: 99-106

https://doi.org/10.2754/avb202695010099

Does the application of a protective microbial culture influence the quality of cooked fish sausages?

Miroslav Jůzl1, Libor Kalhotka1, Veronika Švehlová1, Lukáš Harabiš2, Jan Mareš2, Šárka Nedomová1, Petr Kouřil1, Romana Veselá1, Markéta Janík Piechowiczová1, Aneta Kocandová1, Jan Slováček1

1Mendel University in Brno, Faculty of AgriSciences, Department of Food Technology, Brno, Czech Republic
2Mendel University in Brno, Faculty of AgriSciences, Department of Zoology, Fisheries, Hydrobiology and Apiculture, Brno, Czech Republic

Received October 12, 2025
Accepted March 2, 2026

References

1. AOAC 2016: Official methods of analysis of AOAC International, 20th edition. Gaithersburg, MD, USA: Association of Official Analytical Chemists
2. Bobko M, Mesárošová A, Kročko M, Hanuska A, Bobková A, Poláková K, Vörösová D, Švecová T, Jurčaga L 2025: Cascara, the unusual antioxidant option for meat industry. J Microbiol Biotechnol Food Sci 14: e11662 <https://doi.org/10.55251/jmbfs.11662>
3. Chaillou S, Christieans S, Rivollier M, Lucquin I, Champomier-Vergčs MS, Zagorec M 2014: Quantification and efficiency of Lactobacillus sakei strain mixtures used as protective cultures in ground beef. Meat Sci 97: 332-338 <https://doi.org/10.1016/j.meatsci.2013.08.009>
4. Di Gioia D, Mazzola G, Nikodinoska I, Aloisio I, Langerholc T, Rossi M, Raimondi S, Melero, Rovira J 2016: Lactic acid bacteria as protective cultures in fermented pork meat to prevent Clostridium spp. growth. Int J Food Microbiol 235: 53-59 <https://doi.org/10.1016/j.ijfoodmicro.2016.06.019>
5. Dušková M, Morávková M, Mrázek J, Florianová M, Vorlová L, Karpíšková R 2020: Assessment of antibiotic resistance in starter and non-starter lactobacilli of food origin. Acta Vet Brno 89: 401-411 <https://doi.org/10.2754/avb202089040401>
6. Dušková M, Dorotíková K, Macharáčková B, Ježek F, Kameník J, Šedo O 2024: The shelf life of cooked sausages with reduced salt content. Acta Vet Brno 93: 115-121 <https://doi.org/10.2754/avb202493010115>
7. EAGRI 2021: Food Safety and Nutrition Strategy 2030, Ministry of Agriculture of the Czech Republic Website, Available at: https://www.bezpecnostpotravin.cz/UserFiles/publikace1/Strategie_EN_web.pdf. Last modified March 29, 2021. Accessed September 11, 2025
8. EFSA and ECDC 2024: The European Union One Health 2023 zoonoses report. EFSA Journal 22: e9106
9. Jůzl M, Kameník J, Saláková A, Macharáčková B, Ježek F, Janík Piechowiczová M, Slováček J 2025: The effect of salt reduction on sensory, physicochemical, and microbial quality in selected meat products. Foods 14: 3150 <https://doi.org/10.3390/foods14183150>
10. Jůzl M, Kalhotka L, Kameník J, Dušková M, Ondruchová S, Slováček J 2026: Protective cultures applied in meat products: Technological functions, safety aspects and current advances: A review. Processes 14: 425 <https://doi.org/10.3390/pr14030425>
11. Kalhotka L, Cwiková O, Čírtková V, Matoušová Z, Přichystalová J 2012: Changes in counts of microorganisms and biogenic amines production during the manufacture of fermented sausages Poličan. J Microbiol Biotechnol Food Sci 2: 667-683
12. Komora N, Maciel C, Amaral RA, Fernandes R, Castro SM, Saraiva JA, Teixeira P 2021: Innovative hurdle system towards Listeria monocytogenes inactivation in a fermented meat sausage model - high pressure processing assisted by bacteriophage P100 and bacteriocinogenic Pediococcus acidilactici. Food Res Int 148: 110628 <https://doi.org/10.1016/j.foodres.2021.110628>
13. Holzapfel WH, Geisen R, Schillinger U 1995: Biological preservation of foods with reference to protective cultures, bacteriocins and food-grade enzymes. Int J Food Microbiol 24: 343-362 <https://doi.org/10.1016/0168-1605(94)00036-6>
14. Neves BV, Fernandes AS, Nass P, Jacob-Lopes E, Zepka LQ, Mazzo TM, Longo E, Braga ARC, de Rosso VV 2025: Exploring different food-grade bigel systems for delivering bioactive carotenoids: Part 1 - Evaluation of color parameters. Food Hydrocoll 169: 111625 <https://doi.org/10.1016/j.foodhyd.2025.111625>
15. Xu MM, Kaur M, Pillidge CJ, Torle PJ 2021: Evaluation of the potential of protective cultures to extend the microbial shelf-life of chilled lamb meat. Meat Sci 181: 108613 <https://doi.org/10.1016/j.meatsci.2021.108613>
16. Zakrzewski AJ, Gajewska J, Chajęcka-Wierzchowska W, Załuski D, Zadernowska A 2024: Prevalence of Listeria monocytogenes and other Listeria species in fish, fish products and fish processing environment: A systematic review and meta-analysis. Sci Total Environ 907: 167912 <https://doi.org/10.1016/j.scitotenv.2023.167912>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Archive