Acta Vet. Brno 2026, 95: 215-224

https://doi.org/10.2754/avb202695030215

Effect of palmitic acid nanoparticles on the quality of cryopreserved semen from Holstein bulls

Mohammed A. Abdel-Ghani1, Mohamed E. El-Sharawy2, Hadeer M. Elsayed2, Abd El-Salam M. Metwally2, Ahmed M. Shehabeldin3, Sara M. Ali4

1King Faisal University, College of Veterinary Medicine, Department of Clinical Sciences, Al-Ahsa, Saudi Arabia
2Kafrelsheikh University, Faculty of Agriculture, Department of Animal Production, Kafrelsheikh, Egypt
3Agricultural Research Center, Animal Production Research Institute, Giza, Egypt
4Assiut National University, Faculty of Medicine, Assiut, Egypt

Received June 8, 2026
Accepted September 7, 2026

References

1. Abdel-Ghani MA, El-Sharawy ME, Zaid EK, Shehabeldin AM, Dessouki SM, Moustapha ME, Metwally AESM, El-Shamaa IS 2024: Low concentrations of soybean lecithin nanoparticles had a positive impact on Holstein bulls’ cryopreserved semen. Reprod Domest Anim 59: e14613 <https://doi.org/10.1111/rda.14613>
2. Amirjannati N, Henkel R, Hosseini E, Choopanian P, Moghadasfar H, Arjmand B, Asgharpour SL, Haji PA, Gilany K 2023: The amino acid profile in seminal plasma of normozoospermic men: A correlation analysis with spermiogram parameters and total antioxidant capacity. J Reprod Infert 24: 257-268
3. Andersen JM, Rønning PO, Herning H, Bekken SD, Haugen TB, Witczak O 2016: Fatty acid composition of spermatozoa is associated with BMI and with semen quality. Andrology 4: 857-865 <https://doi.org/10.1111/andr.12227>
4. Beeharry N, Chambers JA, Green IC 2004: Fatty acid protection from palmitic acid-induced apoptosis is lost following PI3-kinase inhibition. Apoptosis 9: 599-607 <https://doi.org/10.1023/B:APPT.0000038039.82506.0c>
5. Cabrillana ME, Monclus MA, Lancellotti TES, Boarelli PV, Vincenti AE, Fornés MM, Sanabria EA, Fornés MW 2017: Thiols of flagellar proteins are essential for progressive motility in human spermatozoa. Reprod Fertil Dev 29: 1435-1446 <https://doi.org/10.1071/RD16225>
6. Carta G, Murru E, Lisai S, Sirigu A, Piras A, Collu M, Batetta B, Gambelli L, Banni S 2015: Dietary triacylglycerols with palmitic acid in the sn-2 position modulate levels of N-acylethanolamides in rat tissues. PLoS ONE 10: e0120424 <https://doi.org/10.1371/journal.pone.0120424>
7. Hu FQ, Jiang SP, Du Y Z, Yuan H, Ye Y Q, Zeng S 2005: Preparation and characterization of stearic acid nanostructured lipid carriers by solvent diffusion method in an aqueous system. Colloids Surf B: Biointerfaces 45: 167-173 <https://doi.org/10.1016/j.colsurfb.2005.08.005>
8. Iranpour FG, Nasr-Esfahani MH, Valojerdi MR, Al-Taraihi TMT 2000: Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet 17: 60-66 <https://doi.org/10.1023/A:1009406231811>
9. Jaswal JS, Keung W, Wang W, Ussher JR, Lopaschuk GD 2011: Targeting fatty acid and carbohydrate oxidation – A novel therapeutic intervention in the ischemic and failing heart. Biochim Biophys Acta 1813: 1333-1350 <https://doi.org/10.1016/j.bbamcr.2011.01.015>
10. Kano C 2018: Effects of semen preservation procedure in egg yolk-tris based extender on bull sperm characteristics. In: Evaluation of bovine semen quality based on sperm organelle functions and motility subpopulation structures. Hokkaido University, Hokkaido, Japan, Chapter II; pp 18-29
11. Kiernan M, Fahey AG, Fair S 2013: The effect of the in vitro supplementation of exogenous long-chain fatty acids on bovine sperm cell function. Reprod Fertil Dev 25: 947-954 <https://doi.org/10.1071/RD12204>
12. Kopeika J, Thornhill A, Khalaf Y 2015: The effect of cryopreservation on the genome of gametes and embryos: Principles of cryobiology and critical appraisal of the evidence. Hum Reprod Up 21: 209-227 <https://doi.org/10.1093/humupd/dmu063>
13. Larsen L, Scheike T, Jensen TK, Bonde JP, Ernst E, Hjollund NH, Zhou Y, Skakkebaek NE, Giwercman A 2000: Computer-assisted semen analysis parameters as predictors for fertility of men from the general population. The Danish First Pregnancy Planner Study Team. Hum Reprod 7: 1562-1567 <https://doi.org/10.1093/humrep/15.7.1562>
14. Librán-Pérez M, Pereiro P, Figueras A, Novoa B 2019: Antiviral activity of palmitic acid via autophagic flux inhibition in zebrafish (Danio rerio). Fish Shellfish Immunol 95: 595-605 <https://doi.org/10.1016/j.fsi.2019.10.055>
15. Martínez-Soto JC, Domingo JC, Cordobilla B, Nicolás M, Fernández L, Albero P, Gadea J, Landeras J 2016: Dietary supplementation with docosahexaenoic acid (DHA) improves seminal antioxidant status and decreases sperm DNA fragmentation. Syst Biol Reprod Med 62: 387-395 <https://doi.org/10.1080/19396368.2016.1246623>
16. Maulana T, Said S, Fidriyanto R, Arifiantini RI, Hasbi H, Jakaria J, Gunawan A 2025: A comparative study of seminal plasma and sperm metabolome profiles in Toraya buffalo bulls. Vet Med Int 2025: 9987127 <https://doi.org/10.1155/vmi/9987127>
17. Murthy AK 2007: Nanoparticles in modern medicine: State of the art and future challenges. Int J Nanomed 2: 129-141
18. Nagy Á, Polichronopoulos T, Gáspárdy A, Solti L, Cseh S 2015: Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis. Acta Vet Hung 63: 370-381 <https://doi.org/10.1556/004.2015.035>
19. Safarinejad MR, Hosseini SY, Dadkhah F, Asgari MA 2010: Relationship of omega-3 and omega-6 fatty acids with semen characteristics, and anti-oxidant status of seminal plasma: a comparison between fertile and infertile men. Clin Nutr 29: 100-105 <https://doi.org/10.1016/j.clnu.2009.07.008>
20. Singh AK, Kaur H, Cheema RS, Kumar A, Honparkhe M, Singh P 2019: Palmitic acid supplementation in tris extender can improve the freezability of buffalo bull semen. Ind Vet J 96: 14-16
21. Van Tran L, Malla BA, Kumar S, Tyagi AK 2017: Polyunsaturated fatty acids in male ruminant reproduction - a review. Asian-Australas J Anim Sci 30: 622-637 <https://doi.org/10.5713/ajas.15.1034>
22. Yánez-Ortiz I, Catalán J, Rodríguez-Gil JE, Miró J, Yeste M 2022: Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep. Anim Reprod Sci 246: 106904 <https://doi.org/10.1016/j.anireprosci.2021.106904>
23. Zhu Z, Li R, Feng, C, Liu, R, Zheng Y, Hoque SAM, Wu D, Lu H, Zhang T, Zeng W 2020: Exogenous oleic acid and palmitic acid improve boar sperm motility via enhancing mitochondrial Β-oxidation for ATP generation. Animals (Basel) 10: 591 <https://doi.org/10.3390/ani10040591>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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