Acta Vet. Brno 2026, 95: 245-250

https://doi.org/10.2754/avb202695030245

Evaluation of Flukefinder® and coproantigen ELISA as alternatives to sedimentation for detecting Fasciola hepatica in sheep

Rajaa Mohmmad Salih1ID, Maab Ibrahim Al-Farwachi2ID

1Veterinary Hospital in Nineveh, Veterinary Directorate, Mosul, Iraq
2University of Mosul, College of Veterinary Medicine, Department of Internal and Preventive Medicine, Mosul, Iraq

Received April 4, 2025
Accepted September 7, 2026

References

1. Al-Alo AZ, Albishtue AA, A-Dujaily AH 2019: Retrospective survey of liver flukes in sheep and cattle based on abattoir data in Al-Najaf Province, Iraq. ASVS 1: 02-08
2. Andrews SJ, Cwiklinski K, Dalton JP 2021: The discovery of Fasciola hepatica and its life cycle. In: Dalton JP (Ed): Fasciolosis. 2nd edn, CABI Publishing, Wallingford, pp. 1-15
3. Alstedt U, Voigt K, Jäger MC, Knubben-Schweizer G, Zablotski Y, Strube C, Wenzel C 2022: Rumen and liver fluke infections in sheep and goats in northern and southern Germany. Animals (Basel) 12: 876 <https://doi.org/10.3390/ani12070876>
4. Bargues MD, Angles R, Coello J, Artigas P, Funatsu IR, Cuervo PF, Buchon P, Mas-Coma S 2021: One Health initiative in the Bolivian Altiplano human fascioliasis hyperendemic area: Lymnaeid biology, population dynamics, microecology and climatic factor influences. Rev Bras Parasitol Vet 30: e025620 <https://doi.org/10.1590/s1984-29612021014>
5. Baratloo A, Hosseini M, Negida A, El Ashal G 2015: Part 1: Simple definition and calculation of accuracy, sensitivity and specificity. Emergency (Tehran, Iran) 3: 48-49
6. Becker AC, Kraemer A, Epe C, Strube C 2016: Sensitivity and efficiency of selected coproscopical methods-sedimentation, combined zinc sulfate sedimentation-flotation, and McMaster method. Parasitol Res 115: 2581-2587 <https://doi.org/10.1007/s00436-016-5003-8>
7. Berhe G, Berhane K, Tadesse G 2009: Prevalence and economic significance of fasciolosis in cattle in Mekelle Area of Ethiopia. Trop Anim Health Prod 41: 1503-1504 <https://doi.org/10.1007/s11250-009-9339-2>
8. Bosco A, Ciuca L, Maurelli M P, Vitiello P, Cringoli G, Prada J M, Rinaldi L 2023: Comparison of Mini-FLOTAC Flukefinder® and sedimentation techniques for detection and quantification of Fasciola hepatica and Calicophoron daubneyi eggs using spiked and naturally infected bovine faecal samples. Parasit Vectors 16: 260 <https://doi.org/10.1186/s13071-023-05890-2>
9. Calvopina M, Romero-Alvarez D, Diaz F, Cevallos W, Sugiyama H 2018: A comparison of Kato-Katz technique to three other methods for diagnosis of Amphimerus spp. liver fluke infection and the prevalence of infection in Chachi Amerindians of Ecuador. PloS One 13: e0203811 <https://doi.org/10.1371/journal.pone.0203811>
10. Conceição MA, Durão RM, Costa IH, da Costa JM 2002: Evaluation of a simple sedimentation method (modified McMaster) for diagnosis of bovine fascioliosis. Vet Parasitol 105: 337-343 <https://doi.org/10.1016/S0304-4017(02)00016-X>
11. Delgado N U, Pereira A E, Martínez R A, Muñoz A A F, Pinilla J C 2023: Seroprevalence and coprological prevalence of liver fluke Fasciola hepatica in cattle and sheep from Santander department, Colombia. Rev Bras Parasitol Vet 32: e009923 <https://doi.org/10.1590/s1984-29612023071>
12. Düwel D, Reisenleiter R 1990: Fasciola hepatica: coprological diagnosis in comparison to the worm burden in sheep and cattle. Angew Parasitol 31: 211-217
13. Fleiss JL, Levin B, Paik MC 2003: Statistical methods for rates and proportions. Wiley, New York, 798 p.
14. Hammami I, Amdouni Y, Romdhane R, Sassi L, Farhat N, Rekik M, Gharbi M 2024: Prevalence of Fasciola hepatica infection in slaughtered sheep from Northwest Tunisia and its risk factors: Association with gastrointestinal helminths infection and anaemia. Vet Med Sci 10: e1575 <https://doi.org/10.1002/vms3.1575>
15. Hassan NA, Diab M, Bayoumi AM, Zidan SA, Hadad G 2025: Review on fascioliasis in animals and human. New Valley Vet J 5: 69-79 <https://doi.org/10.21608/nvvj.2025.323066.1053>
16. Hayward AD, Skuce PJ, McNeilly TN 2021: The influence of liver fluke infection on production in sheep and cattle: a meta-analysis. Int J Parasitol 51: 913-924 <https://doi.org/10.1016/j.ijpara.2021.02.006>
17. Hendrix CM, Robinson CVT 2023: Diagnostic Parasitology for Veterinary Technicians. 6th edn, Maryland Heights, Elsevier Health Sciences, 117 p.
18. Howell A 2024: Update on liver fluke in sheep and cattle. Livestock 29: 162-166 <https://doi.org/10.12968/live.2024.0007>
19. Kahl A, von Samson-Himmelstjerna G, Helm CS, Hodgkinson J, Williams D, Weiher W, Terhalle W, Steuber S, Krücken J 2023: Coproscopical diagnosis of patent Fasciola hepatica infections in sheep—A comparison between standard sedimentation, FLUKEFINDER® and a combination of both. Vet Parasitol 319: 109956 <https://doi.org/10.1016/j.vetpar.2023.109956>
20. Kajugu PE, Hanna RE, Edgar HW, McMahon C, Cooper M, Gordon A, Barley JP, Malone FE, Brennan GP, Fairweather I 2015: Fasciola hepatica: Specificity of a coproantigen ELISA test for diagnosis of fasciolosis in faecal samples from cattle and sheep concurrently infected with gastrointestinal nematodes, coccidians and/or rumen flukes (paramphistomes), under field conditions. Vet Parasitol 212: 181-187 <https://doi.org/10.1016/j.vetpar.2015.07.018>
21. Kamaludeen J, Graham-Brown J, Stephens N, Miller J, Howell A, Beesley NJ, Hodgkinson J, Learmount J, Williams D 2019: Lack of efficacy of triclabendazole against Fasciola hepatica is present on sheep farms in three regions of England, and Wales. Vet Rec 184: 502 <https://doi.org/10.1136/vr.105209>
22. Kurnianto H, Ramanoon SZ, Aziz NAA, Indarjulianto S 2022: Prevalence, risk factors, and infection intensity of fasciolosis in dairy cattle in Boyolali, Indonesia. Vet World 15: 1438-1448 <https://doi.org/10.14202/vetworld.2022.1438-1448>
23. Lan Z, Zhang XH, Xing JL, Zhang AH, Wang HR, Zhang XC, Gao JF, Wang CR 2024: Global prevalence of liver disease in human and domestic animals caused by Fasciola: A systematic review and meta-analysis. J Glob Health 14: 04223 <https://doi.org/10.7189/jogh.14.04223>
24. Mezo M, González-Warleta M, Carro C, Ubeira FM 2004: An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3). J Parasitol 90: 845-852 <https://doi.org/10.1645/GE-192R>
25. Mikaeel FB 2020: Prevalence of Fasciola hepatica in goats and sheep by using ELISA in sera and milk in Duhok, Iraq. Iraqi J Vet Med 44: 113-119 <https://doi.org/10.30539/ijvm.v44i2.983>
26. Modabbernia G, Meshgi B, Kinsley AC 2024: Climatic variations and Fasciola: a review of impacts across the parasite life cycle. Parasitol Res 123: 300 <https://doi.org/10.1007/s00436-024-08319-6>
27. Mungube EO, Bauni SM, Tenhagen BA, Wamae LW, Nginyi JM, Mugambi JM 2006: The prevalence and economic significance of Fasciola gigantica and Stilesia hepatica in slaughtered animals in the semi-arid coastal Kenya. Trop Anim Health Prod 38: 475-483 <https://doi.org/10.1007/s11250-006-4394-4>
28. Munita MP, Rea R, Martinez-Ibeas AM, Byrne N, Kennedy A, Sekiya M, Mulcahy G, Sayers R 2019: Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle. BMC Vet Res 15: 414 <https://doi.org/10.1186/s12917-019-2160-x>
29. Novobilský A, Solis NA, Skarin M, Höglund J. 2016: Assessment of flukicide efficacy against Fasciola hepatica in sheep in Sweden in the absence of a standardised test. Int J Parasitol Drugs Drug Resist 6: 141-147 <https://doi.org/10.1016/j.ijpddr.2016.06.004>
30. Nur Hafizah S, Noor Izani NJ, Ahmad Najib M, Wan-Nor-Amilah WAW 2023: Immunodiagnosis of fascioliasis in ruminants by ELISA Method: A Mini-Review. Malays J Med Sci 30: 25-32
31. Olaogun SC, Byaruhanga C, Ochai SO, Fosgate GT, Marufu MC 2022: Comparison of three diagnostic methods to detect the occurrence of Fasciola species in communally grazed cattle in the North West Province, South Africa. Pathogens 11: 1398 <https://doi.org/10.3390/pathogens11121398>
32. Palmer D, Lyon J, Palmer M, Forshaw D 2014: Evaluation of a copro-antigen ELISA to detect Fasciola hepatica infection in sheep, cattle and horses. Aust Vet J 92: 357-361 <https://doi.org/10.1111/avj.12224>
33. Ploeger HW, Ankum L, Moll L, van Doorn DCK, Mitchell G, Skuce PJ, Zadoks RN, Holzhauer M 2017: Presence and species identity of rumen flukes in cattle and sheep in the Netherlands. Vet Parasitol 30: 42-46 <https://doi.org/10.1016/j.vetpar.2017.06.009>
34. Sargison ND, Scott PR 2011: Diagnosis and economic consequences of triclabendazole resistance in Fasciola hepatica in a sheep flock in south-east Scotland. Vet Rec 168: 159 <https://doi.org/10.1136/vr.c5332>
35. Stuen S, Ersdal C 2022: Fasciolosis – an increasing challenge in the sheep industry. Animals (Basel) 12: 1491 <https://doi.org/10.3390/ani12121491>
36. Takeuchi-Storm N, Denwood M, Hansen TVA, Halasa T, Rattenborg E, Boes J, Enemark HL, Thamsborg SM 2017: Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods. Parasit Vectors 10: 555 <https://doi.org/10.1186/s13071-017-2504-y>
37. Zárate-Rendón D A, Vlaminck J, Levecke B, Briones-Montero A, Geldhof P 2019: Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the detection and quantification of Fasciola hepatica eggs in artificially spiked human stool. Am J Trop Med Hyg 101: 59-61 <https://doi.org/10.4269/ajtmh.18-0988>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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