Acta Vet. Brno 2026, 95: 215-224
Effect of palmitic acid nanoparticles on the quality of cryopreserved semen from Holstein bulls
The purpose of the current study was to investigate the effects of palmitic acid-loaded nanoparticles (PAnano) concentrations on Holstein cryopreserved semen. Semen was diluted, equilibrated, and cryopreserved using control extenders and different PAnano concentrations (2, 4, and 6 µl/ml). Semen was analysed for sperm characteristics and kinetics. The results demonstrated that after dilution, progressive sperm motility (PM), viability, and acrosome integrity percentages did not differ (P > 0.05) among the control and different PAnano concentrations. After equilibration, although the PM, viability, normal morphology, membrane integrity, and intact acrosome values at 4 µl/ml were consistently greater than those of the control, 2, and 6 µl/ml, there were no changes (P > 0.05) between the control and different concentrations of PAnano. After thawing, compared to the control and 2 and 6 µl/ml groups, the PM, membrane integrity, and intact acrosome values at 4 µl/ml were greater (P < 0.05). The mitochondrial potential values were higher at 4 and 6 µl/ml than at the control and 2 µl/ml. Curvilinear velocity values were higher at 4 µl/ml (P < 0.05) than at 2 and 6 µl/ml. Straight line velocity was higher (P < 0.05) at 4 µl/ml compared to 2 and 6 µl/ml values. Compared to 2 and 6 µl/ml, non progressive motility values were lower (P < 0.05) at 4 µl/ml. In conclusion, 4 µl/ml PAnano enhanced sperm characteristics and kinetics, whereas high doses had an adverse effect on sperm kinetics and traits.
Keywords
Cattle, reproduction, fertility, sperm characteristics, cryodamage.
Funding
This work was supported by Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, (KFU 264543).
