Acta Vet. Brno 2026, 95: 245-250
https://doi.org/10.2754/avb202695030245
Evaluation of Flukefinder® and coproantigen ELISA as alternatives to sedimentation for detecting Fasciola hepatica in sheep
References
1. AZ, Albishtue AA, A-Dujaily AH 2019: Retrospective survey of liver flukes in sheep and cattle based on abattoir data in Al-Najaf Province, Iraq. ASVS 1: 02-08
2. Andrews SJ, Cwiklinski K, Dalton JP 2021: The discovery of Fasciola hepatica and its life cycle. In: Dalton JP (Ed): Fasciolosis. 2nd edn, CABI Publishing, Wallingford, pp. 1-15
3. U, Voigt K, Jäger MC, Knubben-Schweizer G, Zablotski Y, Strube C, Wenzel C 2022: Rumen and liver fluke infections in sheep and goats in northern and southern Germany. Animals (Basel) 12: 876
<https://doi.org/10.3390/ani12070876>
4. MD, Angles R, Coello J, Artigas P, Funatsu IR, Cuervo PF, Buchon P, Mas-Coma S 2021: One Health initiative in the Bolivian Altiplano human fascioliasis hyperendemic area: Lymnaeid biology, population dynamics, microecology and climatic factor influences. Rev Bras Parasitol Vet 30: e025620
<https://doi.org/10.1590/s1984-29612021014>
5. A, Hosseini M, Negida A, El Ashal G 2015: Part 1: Simple definition and calculation of accuracy, sensitivity and specificity. Emergency (Tehran, Iran) 3: 48-49
6. AC, Kraemer A, Epe C, Strube C 2016: Sensitivity and efficiency of selected coproscopical methods-sedimentation, combined zinc sulfate sedimentation-flotation, and McMaster method. Parasitol Res 115: 2581-2587
<https://doi.org/10.1007/s00436-016-5003-8>
7. G, Berhane K, Tadesse G 2009: Prevalence and economic significance of fasciolosis in cattle in Mekelle Area of Ethiopia. Trop Anim Health Prod 41: 1503-1504
<https://doi.org/10.1007/s11250-009-9339-2>
8. A, Ciuca L, Maurelli M P, Vitiello P, Cringoli G, Prada J M, Rinaldi L 2023: Comparison of Mini-FLOTAC Flukefinder® and sedimentation techniques for detection and quantification of Fasciola hepatica and Calicophoron daubneyi eggs using spiked and naturally infected bovine faecal samples. Parasit Vectors 16: 260
<https://doi.org/10.1186/s13071-023-05890-2>
9. M, Romero-Alvarez D, Diaz F, Cevallos W, Sugiyama H 2018: A comparison of Kato-Katz technique to three other methods for diagnosis of Amphimerus spp. liver fluke infection and the prevalence of infection in Chachi Amerindians of Ecuador. PloS One 13: e0203811
<https://doi.org/10.1371/journal.pone.0203811>
10. MA, Durão RM, Costa IH, da Costa JM 2002: Evaluation of a simple sedimentation method (modified McMaster) for diagnosis of bovine fascioliosis. Vet Parasitol 105: 337-343
<https://doi.org/10.1016/S0304-4017(02)00016-X>
11. N U, Pereira A E, Martínez R A, Muñoz A A F, Pinilla J C 2023: Seroprevalence and coprological prevalence of liver fluke Fasciola hepatica in cattle and sheep from Santander department, Colombia. Rev Bras Parasitol Vet 32: e009923
<https://doi.org/10.1590/s1984-29612023071>
12. D, Reisenleiter R 1990: Fasciola hepatica: coprological diagnosis in comparison to the worm burden in sheep and cattle. Angew Parasitol 31: 211-217
13. Fleiss JL, Levin B, Paik MC 2003: Statistical methods for rates and proportions. Wiley, New York, 798 p.
14. I, Amdouni Y, Romdhane R, Sassi L, Farhat N, Rekik M, Gharbi M 2024: Prevalence of Fasciola hepatica infection in slaughtered sheep from Northwest Tunisia and its risk factors: Association with gastrointestinal helminths infection and anaemia. Vet Med Sci 10: e1575
<https://doi.org/10.1002/vms3.1575>
15. NA, Diab M, Bayoumi AM, Zidan SA, Hadad G 2025: Review on fascioliasis in animals and human. New Valley Vet J 5: 69-79
<https://doi.org/10.21608/nvvj.2025.323066.1053>
16. AD, Skuce PJ, McNeilly TN 2021: The influence of liver fluke infection on production in sheep and cattle: a meta-analysis. Int J Parasitol 51: 913-924
<https://doi.org/10.1016/j.ijpara.2021.02.006>
17. Hendrix CM, Robinson CVT 2023: Diagnostic Parasitology for Veterinary Technicians. 6th edn, Maryland Heights, Elsevier Health Sciences, 117 p.
18. A 2024: Update on liver fluke in sheep and cattle. Livestock 29: 162-166
<https://doi.org/10.12968/live.2024.0007>
19. A, von Samson-Himmelstjerna G, Helm CS, Hodgkinson J, Williams D, Weiher W, Terhalle W, Steuber S, Krücken J 2023: Coproscopical diagnosis of patent Fasciola hepatica infections in sheep—A comparison between standard sedimentation, FLUKEFINDER® and a combination of both. Vet Parasitol 319: 109956
<https://doi.org/10.1016/j.vetpar.2023.109956>
20. PE, Hanna RE, Edgar HW, McMahon C, Cooper M, Gordon A, Barley JP, Malone FE, Brennan GP, Fairweather I 2015: Fasciola hepatica: Specificity of a coproantigen ELISA test for diagnosis of fasciolosis in faecal samples from cattle and sheep concurrently infected with gastrointestinal nematodes, coccidians and/or rumen flukes (paramphistomes), under field conditions. Vet Parasitol 212: 181-187
<https://doi.org/10.1016/j.vetpar.2015.07.018>
21. J, Graham-Brown J, Stephens N, Miller J, Howell A, Beesley NJ, Hodgkinson J, Learmount J, Williams D 2019: Lack of efficacy of triclabendazole against Fasciola hepatica is present on sheep farms in three regions of England, and Wales. Vet Rec 184: 502
<https://doi.org/10.1136/vr.105209>
22. H, Ramanoon SZ, Aziz NAA, Indarjulianto S 2022: Prevalence, risk factors, and infection intensity of fasciolosis in dairy cattle in Boyolali, Indonesia. Vet World 15: 1438-1448
<https://doi.org/10.14202/vetworld.2022.1438-1448>
23. Z, Zhang XH, Xing JL, Zhang AH, Wang HR, Zhang XC, Gao JF, Wang CR 2024: Global prevalence of liver disease in human and domestic animals caused by Fasciola: A systematic review and meta-analysis. J Glob Health 14: 04223
<https://doi.org/10.7189/jogh.14.04223>
24. M, González-Warleta M, Carro C, Ubeira FM 2004: An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3). J Parasitol 90: 845-852
<https://doi.org/10.1645/GE-192R>
25. FB 2020: Prevalence of Fasciola hepatica in goats and sheep by using ELISA in sera and milk in Duhok, Iraq. Iraqi J Vet Med 44: 113-119
<https://doi.org/10.30539/ijvm.v44i2.983>
26. G, Meshgi B, Kinsley AC 2024: Climatic variations and Fasciola: a review of impacts across the parasite life cycle. Parasitol Res 123: 300
<https://doi.org/10.1007/s00436-024-08319-6>
27. EO, Bauni SM, Tenhagen BA, Wamae LW, Nginyi JM, Mugambi JM 2006: The prevalence and economic significance of Fasciola gigantica and Stilesia hepatica in slaughtered animals in the semi-arid coastal Kenya. Trop Anim Health Prod 38: 475-483
<https://doi.org/10.1007/s11250-006-4394-4>
28. MP, Rea R, Martinez-Ibeas AM, Byrne N, Kennedy A, Sekiya M, Mulcahy G, Sayers R 2019: Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle. BMC Vet Res 15: 414
<https://doi.org/10.1186/s12917-019-2160-x>
29. A, Solis NA, Skarin M, Höglund J. 2016: Assessment of flukicide efficacy against Fasciola hepatica in sheep in Sweden in the absence of a standardised test. Int J Parasitol Drugs Drug Resist 6: 141-147
<https://doi.org/10.1016/j.ijpddr.2016.06.004>
30. S, Noor Izani NJ, Ahmad Najib M, Wan-Nor-Amilah WAW 2023: Immunodiagnosis of fascioliasis in ruminants by ELISA Method: A Mini-Review. Malays J Med Sci 30: 25-32
31. SC, Byaruhanga C, Ochai SO, Fosgate GT, Marufu MC 2022: Comparison of three diagnostic methods to detect the occurrence of Fasciola species in communally grazed cattle in the North West Province, South Africa. Pathogens 11: 1398
<https://doi.org/10.3390/pathogens11121398>
32. D, Lyon J, Palmer M, Forshaw D 2014: Evaluation of a copro-antigen ELISA to detect Fasciola hepatica infection in sheep, cattle and horses. Aust Vet J 92: 357-361
<https://doi.org/10.1111/avj.12224>
33. HW, Ankum L, Moll L, van Doorn DCK, Mitchell G, Skuce PJ, Zadoks RN, Holzhauer M 2017: Presence and species identity of rumen flukes in cattle and sheep in the Netherlands. Vet Parasitol 30: 42-46
<https://doi.org/10.1016/j.vetpar.2017.06.009>
34. ND, Scott PR 2011: Diagnosis and economic consequences of triclabendazole resistance in Fasciola hepatica in a sheep flock in south-east Scotland. Vet Rec 168: 159
<https://doi.org/10.1136/vr.c5332>
35. S, Ersdal C 2022: Fasciolosis – an increasing challenge in the sheep industry. Animals (Basel) 12: 1491
<https://doi.org/10.3390/ani12121491>
36. N, Denwood M, Hansen TVA, Halasa T, Rattenborg E, Boes J, Enemark HL, Thamsborg SM 2017: Farm-level risk factors for Fasciola hepatica infection in Danish dairy cattle as evaluated by two diagnostic methods. Parasit Vectors 10: 555
<https://doi.org/10.1186/s13071-017-2504-y>
37. D A, Vlaminck J, Levecke B, Briones-Montero A, Geldhof P 2019: Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the detection and quantification of Fasciola hepatica eggs in artificially spiked human stool. Am J Trop Med Hyg 101: 59-61
<https://doi.org/10.4269/ajtmh.18-0988>


