Acta Vet. Brno 2006, 75: 419-425

The Effect of Sterilization on Size and Shape of Fat Globules in Model Processed Cheese Samples

B. Tremlová1, P. Štarha2, F. Buňka3, Z. Gistingrová1, J. Hrabě3

1University of Veterinary and Pharmaceutical Sciences Brno
2Brno University of Technology
3Tomáš Baťa University in Zlín

Received November 3, 2005
Accepted May 11, 2006

Model cheese samples from 4 independent productions were heat sterilized (117 °C, 20 minutes) after the melting process and packing with an aim to prolong their durability. The objective of the study was to assess changes in the size and shape of fat globules due to heat sterilization by using image analysis methods. The study included a selection of suitable methods of preparation mounts, taking microphotographs and making overlays for automatic processing of photographs by image analyser, ascertaining parameters to determine the size and shape of fat globules and statistical analysis of results obtained. The results of the experiment suggest that changes in shape of fat globules due to heat sterilization are not unequivocal. We found that the size of fat globules was significantly increased (p < 0.01) due to heat sterilization (117 °C, 20 min), and the shares of small fat globules (up to 500 μm2, or 100 μm2) in the samples of heat sterilized processed cheese were decreased. The results imply that the image analysis method is very useful when assessing the effect of technological process on the quality of processed cheese quality.