Acta Vet. Brno 2008, 77: 423-429

Salinomycin Concentration in Eggs and Tissues of Laying Hens

K. Šinigoj-Gačnik1, O. Zorman Rojs2

1Institute for Food Hygiene and Bromatology
2Institute for Health Care of Poultry Veterinary Faculty, University of Ljubljana, Slovenia

Received August 24, 2006
Accepted April 14, 2008

The objective of our study was to monitor the presence of salinomycin in eggs and tissues of laying hens fed with rations containing 60 mg kg-1 salinomycin sodium for five days. Residues of salinomycin were determined on the day of withdrawal from salinomycin treatment in the breast and thigh muscle, liver, abdominal fat and ovarian yolk. In eggs, residues of salinomycin were monitored in both yolk and albumen daily from the beginning of treatment until the tenth day after withdrawal. Salinomycin was first found in yolk 1 day after starting the treatment and persisted for 8 days after withdrawal. The highest average level, 480 μg kg-1, was present on the third day after withdrawal. In albumen, levels of salinomycin residues were significantly lower, with a maximum level of 15 μg kg-1 reached on the fifth day of the experiment; and they were only found up to one day after withdrawal. Salinomycin was found in all ovarian yolks present in the body of layer hens in a concentration range between 237 and 553 μg kg-1. It was also found in the abdominal fat (concentration range from 62 to 237 μg kg-1) and the liver (concentration ≤ 10 μg kg-1), but not in breast or thigh muscle tissues. No changes in salinomycin residues were observed after cooking and frying the eggs.