Acta Vet. Brno 2009, 78: 151-154

Protein and Antigenic Profile among Mycoplasma bovis Field Strains Isolated in Bosnia and Herzegovina

Maid Rifatbegović1, Patrícia Assunção2, Šemso Pašić1, Christian de la Fe3, Jose B. Poveda2

1Department of Microbiology and Infectious Diseases, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
2Unidad de Epidemiologia y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Arucas, Spain
3Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain

Received December 18, 2007
Accepted November 12, 2008

Mycoplasma bovis is a serious, worldwide-spread but often overlooked pathogen causing respiratory disease, mastitis, and arthritis in cattle. In this study we characterize the protein and antigenic profiles of M. bovis field strains isolated in Bosnia and Herzegovina by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting, and analyze possible variations among these strains. Greater differences occurred when comparing field strains with the reference strain PG45. One field strain isolated from lung samples of a heifer was markedly different from strains isolated from nasal swabs taken from cattle raised in another geographic region. A possible correlation may exist between protein and antigen profiles of M. bovis field strains, geographic regions and anatomical sites of isolation.