Acta Vet. Brno 2014, 83: 3-8

https://doi.org/10.2754/avb201483S10S3

Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk

Lenka Necidová1, Šárka Bursová1, Alena Skočková1, Bohdana Janštová2, Pavla Prachařová1, Žaneta Ševčíková1, Bohumíra Janštová1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Milk Hygiene and Technology, Brno, Czech Republic
2University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Vegetable Foodstuffs Hygiene and Technology, Brno, Czech Republic

Received July 21, 2014
Accepted November 26, 2014

The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.

Funding

The study was supported by research grants MSM 6215712402 Veterinary Aspects of Food Safety and Quality, and IGA VFU 16/2013/FVHE.

References

18 live references

Open full article