Acta Vet. Brno 2014, 83: 71-76

Quantitative immunohistochemical method for detection of wheat protein in model sausage

Zuzana Řezáčová Lukášková1, Matěj Pospiech1, Bohuslava Tremlová1, Eva Renčová2, Michaela Petrášová1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Hygiene and Technology of Vegetable Foodstuff, Brno, Czech Republic
2Veterinary Research Institute, Brno, Czech Republic

Received August 29, 2014
Accepted April 1, 2015

Since gluten can induce coeliac symptoms in hypersensitive consumers with coeliac disease, it is necessary to label foodstuffs containing it. In order to label foodstuffs, it is essential to find reliable methods to accurately determine the amount of wheat protein in food. The objective of this study was to compare the quantitative detection of wheat protein in model sausages by ELISA and immunohistochemical methods. Immunohistochemistry was combined with stereology to achieve quantitative results. High correlation between addition of wheat protein and compared methods was confirmed. For ELISA method the determined values were r = 0.98, P < 0.01; for stereologythe determined values were r = 0.94, P < 0.01. Although ELISA is an accredited method, it was not reliable, unlike immunohistochemical methods (stereology SD = 3.1).


This work was supported by the project of NAZV No. 1B53004, Research Plan of Veterinary Aspects of Foodstuffs Safety and Quality (MŠMT No. 6215712402) and the project of IGA VFU 2009 No. 206/2009/VFU.


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