Quantitative immunohistochemical method for detection of wheat protein in model sausage

Řezáčová Lukášková, Zuzana; Pospiech, Matěj; Tremlová, Bohuslava; Renčová, Eva; Petrášová, Michaela

doi:10.2754/avb201483S10S71

Acta Veterinaria > Archive > 2014, Vol. 83 > Supplementum 10 > Quantitative immunohistochemical method…

Acta Vet. Brno 2014, 83: 71-76

https://doi.org/10.2754/avb201483S10S71

Quantitative immunohistochemical method for detection of wheat protein in model sausage

Zuzana Řezáčová Lukášková¹, Matěj Pospiech¹, Bohuslava Tremlová¹, Eva Renčová², Michaela Petrášová¹

¹University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Hygiene and Technology of Vegetable Foodstuff, Brno, Czech Republic
²Veterinary Research Institute, Brno, Czech Republic

Received August 29, 2014
Accepted April 1, 2015

Since gluten can induce coeliac symptoms in hypersensitive consumers with coeliac disease, it is necessary to label foodstuffs containing it. In order to label foodstuffs, it is essential to find reliable methods to accurately determine the amount of wheat protein in food. The objective of this study was to compare the quantitative detection of wheat protein in model sausages by ELISA and immunohistochemical methods. Immunohistochemistry was combined with stereology to achieve quantitative results. High correlation between addition of wheat protein and compared methods was confirmed. For ELISA method the determined values were r = 0.98, P < 0.01; for stereologythe determined values were r = 0.94, P < 0.01. Although ELISA is an accredited method, it was not reliable, unlike immunohistochemical methods (stereology SD = 3.1).

Keywords

Food allergens, gluten/gliadin, identification, histochemistry, meat products.

Funding

This work was supported by the project of NAZV No. 1B53004, Research Plan of Veterinary Aspects of Foodstuffs Safety and Quality (MŠMT No. 6215712402) and the project of IGA VFU 2009 No. 206/2009/VFU.