Acta Vet. Brno 2017, 86: 159-165

Soapwort extract supplementation alters antioxidant status of serum, liver and heart tissues in growing Japanese quails reared under chronic intermittent cold stress

Bestami Dalkilic1, Mehtap Ozcelik2, Zafer Cambay2, Naci Omer Alayunt3, Ulku Gulcihan Simsek4, Seda Iflazoglu Mutlu5, Mehmet Ciftci5

1University of Gaziantep, Vocational School of Technical Sciences, Department of Plant and Animal Production, Gaziantep, Turkey
2University of Firat, Vocational School of Health Services, Elazig, Turkey
3University of Firat, Faculty of Arts and Sciences, Department of Chemistry, Elazig, Turkey
4University of Firat, Faculty of Veterinary Medicine, Department of Animal Science, Elazig, Turkey
5University of Firat, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Diseases, Elazig, Turkey

Received February 21, 2016
Accepted May 31, 2017

Antioxidant effect of dietary soapwort extract supplementation was studied in growing Japanese quails suffering from chronic intermittent cold stress. For this purpose, a total of ninety 15-d-old quails were divided into three groups with three replicates. Chronic intermittent cold stress was applied every night between 22.00 to 06.00 h; starting at 14 °C for the first week, and gradually weekly lowered to 8 °C. Three groups were fed with corn-soy based standard diets supplemented with 0, 50, and 100 ppm soapwort extract for four weeks. At the end of the study, three males and three females were slaughtered to determine total antioxidant and oxidant status of serum, malondialdehyde, glutathione, glutathione peroxidase activity, superoxide dismutase of liver and heart tissues. Although the dietary soapwort extract had no effect on serum total antioxidant capacity, it significantly lowered the total oxidant status of serum in cold stressed quails. Glutathione and superoxide dismutase enzyme activity of liver and heart tissues were similar among groups. While the dietary soapwort extract had no effect on glutathione peroxidase activity of the heart tissue, it significantly increased glutathione peroxidase activity in the liver tissue. In relation to the control group, malondialdehyde concentrations in the liver and heart tissues were significantly lower in soapwort extract groups. These data suggest that dietary soapwort extract could alleviate the detrimental effects of oxidative stress in growing Japanese quails exposed to cold stress.


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