Acta Vet. Brno 2019, 88: 473-480

The content of selected vitamins and iodine in mare’s milk

Pavlína Navrátilová, Ivana Borkovcová, Lenka Kaniová, Sandra Dluhošová, Hana Zachovalová

University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Milk Hygiene and Technology, Brno, Czech Republic

Received February 27, 2019
Accepted October 29, 2019

The aim of the study was to contribute to scarce literature data on the content of selected vitamins and iodine in mare’s milk. The study focused on the content of selected lipophilic vitamins (A, E), hydrophilic vitamins B1, B2, B6) and iodine in milk samples obtained from 8 mares during 6 months of lactation. The content of micronutrients was evaluated according to the stage of lactation. Vitamins B2 and B6 were determined using ion-pair reversed phase high performance liquid chromatography with fluorescence detection, vitamin B1 by fluorescence detection by reversed-phase liquid chromatography, vitamins A and E by the ultra-high performance liquid chromatography method with ultraviolet and fluorescence detection and iodine by the spectrophotometric method. Mean concentrations of vitamins A (0.06 ± 0.08 mg/l), E (0.083 ± 0.14 mg/l), B1 (256.24 ± 44.19 µg/l), B2 (111.40 ± 81.88 µg/l), B6 (0.30 ± 0.12 mg/l) reached lower values in mare’s milk compared to cow’s milk. The mean value of iodine in mare’s milk reached 44.48 ± 54.45 µg/l. Lactation stage proved to be a significant factor for vitamin B6 and iodine.


This work was supported by the Internal Grant Agency of the University of Veterinary and Pharmaceutical Sciences Brno [Grant no. 203/2016/FVHE].


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