Acta Vet. Brno 2020, 89: 3-10

https://doi.org/10.2754/avb202089010003

Acid-base balance parameters of follicular fluid and venous blood in cattle

Eva Indrová1, Michaela Andrlikova1, Vladislav Bína2, Radovan Doležel1, Miloslava Lopatářová1, Jana Novakova1, Marta Zavadilová1, Svatopluk Čech1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Ruminant and Swine Clinic, Brno, Czech Republic
2University of Economics, Prague, Faculty of Management, Department of Exact Methods, Jindřichův Hradec, Czech Republic

Received September 27, 2019
Accepted January 28, 2020

The study aimed to compare differences of physiological acid-base balance (ABB) parameters in follicular fluid (FF) and venous blood (VB) and to evaluate ABB parameters in FF collected from different ovarian follicles in dairy cows and heifers. The ABB parameters (pH, pCO2, pO2, HCO3 and base excess (BE)) in the FF of the preovulatory follicle, of the dominant follicle on the 9th day of the cycle and of the superovulatory estrous follicles were compared to VB. Similarly, the dynamics of the ABB profile in FF and VB were monitored in repeated sampling in a group of heifers stimulated by follicle-stimulating hormone (FSH). Higher values of pH and pO2 and lower values of pCO2, HCO3 and BE were found in FF compared to VB in all experiments. Laterality of ovaries, time of sampling, ovarian activity or stimulation of the follicular development by FSH did not significantly influence ABB parameters. We found higher pH (7.392 ± 0.027 vs. 7.364 ± 0.032) and pO2 (13.83 ± 2.20 kPa vs. 4.50 ± 0.67 kPa), lower pCO2 (5.70 ± 0.39 kPa vs. 6.54 ± 0.61 kPa), HCO3 (25.51 ± 1.52 mmol/l vs. 26.86 ± 2.12 mmol/l) and BE (1.14 ± 1.57 mmol/l vs. 1.95 ± 2.2 mmol/l) in FF compared to VB in all non-stimulated cows. Similar relationships between FF and VB were found in all FSH stimulated cows. The study provides as yet unknown knowledge on the physiology of follicular fluid in cattle.

Keywords

BE, follicle, HCO3-, heifer, pCO2, pH.

Funding

The study was supported by the Internal Grant Agency of the University of Veterinary and Pharmaceutical Sciences Brno, No. 120/2018/FVL.

References

28 live references