Acta Vet. Brno 2020, 89: 3-10

Acid-base balance parameters of follicular fluid and venous blood in cattle

Eva Indrová1, Michaela Andrlíková1, Vladislav Bína2, Radovan Doležel1, Miloslava Lopatářová1, Jana Novakova1, Marta Zavadilová1, Svatopluk Čech1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Ruminant and Swine Clinic, Brno, Czech Republic
2University of Economics, Prague, Faculty of Management, Department of Exact Methods, Jindřichův Hradec, Czech Republic

Received September 27, 2019
Accepted January 28, 2020


1. Berg DK, Beaumont SE, Berg MC, Hull CD, Tervit HR 2003: Oxygen and carbon dioxide tension in days 14–15 dominant bovine follicles measured in vivo or 4 hours post-mortem. Theriogenology 59: 406 <>
2. Berg MC, Beaumont SE, Peterson AJ, Berg DK 2005: A procedure combining iSTAT® analysis with OPU to study bovine follicular environments. Reprod Fertil Dev 17: 318-319 <>
3. Boyers SP, Lavy G, Russel JB, De Cherney AH 1987: A paired analysis of in vitro fertilization and cleavage rates of first- versus last-recovered preovulatory human oocytes exposed to varying intervals of 100% CO2 pneumoperitoneum and general anesthesia. Fertil Steril 48: 969-974 <>
4. Cech S, Dolezel R, Lopatarova M, Pechova A 2007: Acid-base balance of follicular fluid in dairy heifers. Reprod Domest Anim 42: 113
5. Cech S, Indrova E, Lopatarova M, Mala J, Pechova A, Dolezel R 2011: Limitations of ultrasound guided follicular aspiration for analysis of follicular fluid in dairy cattle. Acta Vet Brno 80: 179-184 <>
6. Cech S, Dolezel R, Holickova K, Indrova E, Kochova T, Lopatarova M, Mala J, Pechova A, Zavadilova M, Zilka L 2013: A new device for the aspiration of follicular fluid for acid-base balance in cattle. Vet Med 58: 527-534 <>
7. Constable PD 2008: Strong ion difference theory: a revolutionary approach to the diagnosis and treatment of acid-base abnormalities in cattle. Proc. XXV. Jubilee World Buiatrics Congress 28-33
8. Daya S 1988: Follicular fluid pH changes following intraperitoneal exposure of Graafian follicles to carbon dioxide: a comparative study with follicles exposed to ultrasound. Hum Reprod 3: 727-730 <>
9. de Castro e Paula LA, Andrzejewski J, Julian D, Spicer LJ, Hansen PJ, 2008: Oxygen and steroid concentrations in preovulatory follicles of lactating dairy cows exposed to acute heat stress. Theriogenology 69: 805-813 <>
10. Eissa HM 1996: Concentrations of steroids and biochemical constituents in follicular fluid of buffalo cows during different stages of the oestrous cycle. Br Vet J 152: 573-581 <>
11. Fischer B, Kiinzel W, Kleinstein J, Gips H 1992: Oxygen tension in follicular fluid falls with follicle maturation. Eur J Obstet Gynecol Reprod Biol 43: 39-43 <>
12. Gérard N, Loiseau S, Duchamp G, Seguin F 2002: Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H MNR). Reproduction 124: 241-248 <>
13. Hafez ESE 2000: Reproduction in farm animals. 7th edn, Lea a Febiger, Philadelphia, p. 505
14. Hussein HA, Boryczko Z, Bostedt H 2013: Acid-base parameters and steroid concentrations in preovulatory follicles and plasma of lactating dairy cows with spontaneous and synchronized oestrus or follicular cyst. Reprod Domest Anim 48: 833-839 <>
15. Imoedemhe DAG, Chan RCW, Ramadan IAG, Sigue AB 1993: Changes in follicular fluid gas and pH during carbon dioxide pneumoperitoneum for laparoscopic aspiration and their effect on human oocyte fertilizability. Fertil Steril 59: 177-182 <>
16. Indrova E, Dolezel R, Novakova-Mala J, Pechova A, Zavadilova M, Cech S 2017: Impact of acute metabolic acidosis on the acid-base balance in follicular fluid and blood in dairy cattle. Theriogenology 89: 41-46 <>
17. Kohram H, Bousquet D, Durocher J, Guilbault LA 1998: Alteration of follicular dynamics and superovulatory responses by gonadotropin releasing hormone and follicular puncture in cattle: a field trial. Theriogenology 49: 1165-1174 <>
18. Landau S, Braw-Tal R, Kaim M, Bor A, Bruckental I 2000: Preovulatory follicular status and diet affect the insulin and glucose content of follicles in high-yielding dairy cows. Anim Reprod Sci 64: 181-197 <>
19. Moallem U, Folman Y, Bor A, Arav A, Sklan D 2011: Effect of calcium soaps of fatty acids and administration of somatotropin on milk production, preovulatory follicular development, and plasma and follicular fluid lipid composition in high yielding dairy cows. J Dairy Sci 82: 2358-2368 <>
20. Nagy O, Sedovic M, Slanina L 1994: Central and peripheral arterial and venous blood in cattle with respect to acid-base balance evaluations. Vet Med-Czech 39: 1-9
21. Pechova A, Hofirek B, Pavlata L, Dvorak R 2009: Produkční a preventivní medicína v chovech skotu. In: Hofirek B, editor. Nemoci skotu, 1st edn. Brno: Noviko, a.s., p. 1042
22. R Core Team 2017: R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria
23. Redding GP, Bronlund JE, Hart AL 2008: Theoretical investigation into the dissolved oxygen levels in follicular fluid of the developing human follicle using mathematical modeling. Reprod Fertil Dev 20: 408-417 <>
24. Reece WO 2015: Fundamental of acid-base balance. In: Reece W.O, editor. Dukes’ Physiology of Domestic Animals, 13th edn. Cornell University Press, Ithaca, NY, pp. 137-148
25. Shalgi R, Kraicer PF, Soferman N 1972: Gases and electrolytes of human follicular fluid. J Reprod Fertil 28: 335-340 <>
26. Shehab-El-Deen MAMM, Leroy JLMR, Fadel MS, Saleh SYA, Maes D, Van Soom A 2010: Biochemical changes in the follicular fluid of the dominant follicle of high producing dairy cows exposed to heat stress early post-partum. Anim Reprod Sci 117: 189-200 <>
27. Vos PLAM, de Loos FAM, Pieterse MC, Bevers MM, Taverne MAM, Dieleman SJ 1994: Evaluation of transvaginal ultrasound-guided follicle puncture to collect oocytes and follicular fluids at consecutive times relative to the preovulatory LH surge in eCG/ PG-treated cows. Theriogenology 41: 829-840 <>
28. Walters AH, Pryor AW, Bailey TL, Pearson RE, Gwazdauskas FC 2002: Milk yield, energy balance, hormone, follicular and oocyte measures in early and mid-lactation Holstein cows. Theriogenology 57: 949-961 <>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue