Acta Vet. Brno 2020, 89: 79-87

https://doi.org/10.2754/avb202089010079

Measurement of phagocyte activity in heterotherms

Tomas Heger1, Jan Zukal2,3, Veronika Seidlová1, Monika Nemcova1, David Necas4, Ivana Papežíková1, Vladimír Piaček1, Renata Zajíčková2, Hana Banďouchová1, Jiří Pikula1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Brno, Czech Republic
2Czech Academy of Sciences v.v.i., Institute of Vertebrate Biology, Brno, Czech Republic
3Masaryk University, Faculty of Science, Department of Botany and Zoology, Brno, Czech Republic
4Masaryk University, CEITEC, Plasma Technologies, Brno, Czech Republic

Received October 24, 2019
Accepted February 25, 2020

The heterotherm immune system undergoes significant variation in response to life cycle periodicity and torpor. As heterothermic bats are important reservoirs of zoonotic agents and modulation of immune activity can affect host-pathogen interactions, this work aimed at developing a suitable method for assessing heterotherm phagocyte activity. Chemiluminescence measurements were evaluated by mathematical and mechanistic approaches, both of which yielded comparable results in time-related parameters of phagocyte activity. Using a mathematical method, however, we developed a model that can be applied to particular specimens. The proposed equation offers a simple and reliable tool for comparing phagocyte activity, the values of which can be used for further analysis. While time-related parameters of bat phagocyte activity varied with measurement temperature, with the onset of respiratory burst at 38 °C being quicker than at 25 °C, quantitative values ​​of phagocyte activity were not influenced by measurement temperature. Further, homeotherm phagocyte activity parameters were more variable at 25 °C. Considering there was no influence of measurement temperature on the total volume of heterotherm phagocyte activity, we suggest that parameters measured at 25 °C are more representative of the immune status adapted to physiological extremes at low body temperatures.

Funding

This study was supported by the Czech Science Foundation (Grant No. 17-20286S). We are grateful to Dr. Kevin Roche for correction and improvement of the English text.

References

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