Acta Vet. Brno 2015, 84: 365-371

The effect of aloe preparation and 5-oxo-1,2,4-triazine on immunological and haematological indices of blood of turkey hens subjected to stress

Katarzyna Ognik, Iwona Sembratowicz, Anna Czech

University of Life Sciences in Lublin, Faculty of Animal and Breeding, Department of Biochemistry and Toxicology, Lublin, Poland

Received October 27, 2014
Accepted November 10, 2015

The aim of this study was to determine the effect of aloe extract or 5-oxo-1,2,4-triazine on immunological and haematological indices during artificially induced stress in turkey hens. The experiment was carried out on 360 turkey hens (6 groups). The groups C and C+stress were the control groups and did not receive any additive. Birds from the groups A and A+stress were administered aloe extract at the amount of 0.70 ml/kg body weight (bw)/day. The turkey hens from the groups T and T+stress received 5-oxo-1,2,4-triazine at the amount of 30 μg/kg bw/day. The additives were administered in the birds’ drinking water twice for 28 days (days 36–63 and 78–105) of the birds’ life. On days 61–63 and 103–105 a stress factor (simultaneous crowding and a change in temperature and lighting) was introduced in the groups C+stress, A+stress and T+stress for 1 hour a day. The results showed that the applied stress factors caused a significant (P ≤ 0.05) decrease in the lysozyme level and % phagocytic cells, as well as a non-significant reduction in the metabolic activity of heterophils. Under the influence of stress an increase (P ≤ 0.05) in the percentage of monocytes and basophils was observed. The use of aloe preparation improved some indicators of immunity (increased, P ≤ 0.05, lysozyme and % phagocytic cells) in the turkey hens. To alleviate the negative effect of stress on immune reactions, supplementation of diets with aloe extract supplemented with resveratrol and vitamin C may be considered.


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