Acta Vet. Brno 2019, 88: 65-71

The effect of fumonisins producing Fusarium verticillioides on the microbiota in pig caecum

Huu Anh Dang1, Attila Zsolnai1, Melinda Kovács1,2, Brigitta Bóta2, Gábor Mihucz1, Roland Pósa1, Kinga Marosi1, Mariam Kachlek1, Judit Szabó-Fodor2

1Kaposvár University, Faculty of Agricultural and Environmental Sciences, Kaposvár, Hungary
2Kaposvár University, MTA-KE Mycotoxins in the Food Chain Research Group, Kaposvár, Hungary

Received May 30, 2018
Accepted February 12, 2019

Fumonisin-producing fungal species, Fusarium verticillioides, culture was mixed in the diets of 6 piglets for 9 days (Fumonisin B1 [FB1] intake of 17 mg/kg) to investigate whether there is any potential alteration in the caecal bacterial communities between the experimental (with F. verticillioides) and control groups (without F. verticillioides). Plate count agar culturing technique was applied to measure the amount of aerobic and anaerobic bacteria, Escherichia coli, coliforms, Lactobacillus spp. and Clostridium perfringens. A significant difference was observed between the control and experimental group only in the case of aerobic bacteria on Day 4, 8.60 ± 0.22 compared to 8.06 ± 0.20 (P < 0.05), respectively. Quantitative polymerase chain reaction (qPCR) was performed to estimate the DNA copy number of total bacteria, Bacteroides and Prevotella spp., Clostridium spp., E. coli, Enterobacteriales, Firmicutes and Lactobacillus spp. Significant differences were observed between the control and experimental group regarding total bacteria on Day 2 and Day 6, Firmicutes on Day 2 and E. coli and Enterobacteriales on Day 4. Regarding the entire feeding time, no significant difference between the two groups was found in all species of investigated bacteria by the culturing technique and qPCR after an 8-day exposure. The present research contributes to the understanding of how microbiota responds to the FB1 load.


The work was supported by the GINOP-2.3.2-15-2016-00046 and the EFOP-3.6.3-VEKOP-16-2017-00005 programmes.


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