The effect of fumonisins producing <i>Fusarium verticillioides</i> on the microbiota in pig caecum

Dang, Huu Anh; Zsolnai, Attila; Kovács, Melinda; Bóta, Brigitta; Mihucz, Gábor; Pósa, Roland; Marosi, Kinga; Kachlek, Mariam; Szabó-Fodor, Judit

doi:10.2754/avb201988010065

Acta Veterinaria > Archive > 2019, Vol. 88 > Issue 1 > The effect of fumonisins producing…

Acta Vet. Brno 2019, 88: 65-71

https://doi.org/10.2754/avb201988010065

The effect of fumonisins producing Fusarium verticillioides on the microbiota in pig caecum

Huu Anh Dang¹, Attila Zsolnai¹, Melinda Kovács^1,2, Brigitta Bóta², Gábor Mihucz¹, Roland Pósa¹, Kinga Marosi¹, Mariam Kachlek¹, Judit Szabó-Fodor²

¹Kaposvár University, Faculty of Agricultural and Environmental Sciences, Kaposvár, Hungary
²Kaposvár University, MTA-KE Mycotoxins in the Food Chain Research Group, Kaposvár, Hungary

Received May 30, 2018
Accepted February 12, 2019

Fumonisin-producing fungal species, Fusarium verticillioides, culture was mixed in the diets of 6 piglets for 9 days (Fumonisin B₁ [FB₁] intake of 17 mg/kg) to investigate whether there is any potential alteration in the caecal bacterial communities between the experimental (with F. verticillioides) and control groups (without F. verticillioides). Plate count agar culturing technique was applied to measure the amount of aerobic and anaerobic bacteria, Escherichia coli, coliforms, Lactobacillus spp. and Clostridium perfringens. A significant difference was observed between the control and experimental group only in the case of aerobic bacteria on Day 4, 8.60 ± 0.22 compared to 8.06 ± 0.20 (P < 0.05), respectively. Quantitative polymerase chain reaction (qPCR) was performed to estimate the DNA copy number of total bacteria, Bacteroides and Prevotella spp., Clostridium spp., E. coli, Enterobacteriales, Firmicutes and Lactobacillus spp. Significant differences were observed between the control and experimental group regarding total bacteria on Day 2 and Day 6, Firmicutes on Day 2 and E. coli and Enterobacteriales on Day 4. Regarding the entire feeding time, no significant difference between the two groups was found in all species of investigated bacteria by the culturing technique and qPCR after an 8-day exposure. The present research contributes to the understanding of how microbiota responds to the FB₁ load.