PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle

Nesvadbová, Michaela; Bořilová, Gabriela; Hulánková, Radka

doi:10.2754/avb201988010103

Acta Veterinaria > Archive > 2019, Vol. 88 > Issue 1 > PCR-RFLP identification of meat from red…

Acta Vet. Brno 2019, 88: 103-111

https://doi.org/10.2754/avb201988010103

PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle

Michaela Nesvadbová^1,2, Gabriela Bořilová^1,2, Radka Hulánková^1,2

¹CEITEC - Central European Institute of Technology, Brno, Czech Republic
²University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Meat Hygiene and Technology, Brno, Czech Republic

Received October 24, 2018
Accepted February 12, 2019

Meat authentication is currently a key topic in relation to the quality and safety of food of animal origin at all levels of production and the global distribution chain. New polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) based on digestion of PCR products with two restriction enzymes, MboII and AciI, have been developed for the specific identification of raw and heat-processed meat from red deer (Cervus elaphus), sika deer (Cervus nippon), roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis musimon), wild boar (Sus scrofa), hare (Lepus europaeus) and cattle (Bos taurus). The PCR primers were targeted in a well-conserved region of the cytochrome b (CYTB) gene to amplify a 378 bp region of all the analysed species. This simple, rapid and cost-effective method is suitable for identification of the meat of game species and their possible substitution by beef.

Keywords

Authentication, game meat, cytochrome b, PCR, restriction enzymes.

Funding

This research was supported by NAZV QJ1530107 of the Ministry of Agriculture of the Czech Republic and carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II.